The using of gamma-octanolactone in the broiler diet: intestinal microbiome

Abstract

It is known that the inclusion of antibiotics in chicken feed leads to the development of resistance in bacteria. It is for this reason that the search for additives to alternative antibiotics becomes relevant. The ability of gamma-glucanolactone to inhibit various quorum systems (QS) LuxI/LuxR in bacteria has been demonstrated. Based on this, it can be assumed that the use of this substance in the diet will reduce infections in the intestines of poultry and improve the effects of broiler rearing. The aim of this work was to evaluate the microbiome of the iliac intestine of broilers when introducing gammaoctanolactone into the diet.The study was conducted using the sequencing method. Total DNA from samples of intestinal contents was isolated using the FastDNA ® Faecal Extraction Kit. Pair-end sequencing of DNA library amplicons was performed on the Illumina MiSeq Reagent Kit platform using the MiSeq v.2 Reagent Kit (500 cycles) (Illumina, San Diego, California, USA). The inclusion of gamma-octalactone in the diet of broilers at a dosage of 0.05 and 0,20 ml / kg w.m. / day contributes to an increase in the number of representatives of the phylum Firmicutes in the iliac intestine of poultry. The dosage of 0,05 ml / kg w.m. / day of gamma-octanolactone reduced the content of the genus Streptococcus by 4,21%; the dosage of 0,20 ml / kg w.m./ day of gammaoctanolactone increased by 10% representatives of the genera Lactobacillus and Turicibacter, relative to the control.

Broiler Chicken Cecal Microbiome and Poultry Farming Productivity: A Meta-Analysis

“…Results of ten experimental series performed during the period of 2018-2023 [14][15][16][17][18][19][20][21][22][23] were included in the meta-analysis (Figure 1). The inclusion criteria were: (i) the study implementation to be in accordance with the ethical statement and the principles of good laboratory practice; (ii) use of broiler chickens aged from 7 to 42 days (experiment duration of 35 days); (iii) keeping birds on a standard basic diet supplemented with various additives; (iv) standard recording of zootechnical data integrated with the European Production Efficiency Factor (EPEF); (v) standard Illumina 16S-RNA gene sequencing technology to characterize the cecal microbiome.…”

“…Seven-day-old broiler chickens were randomly divided into equal replicates and fed with the basic diets intended for the starting period (7-28 days), and growing period (29-42 days). The control group in each series received the basic diet only, while the experimental groups were fed a diet supplemented with Oak Bark extract [14,15,19], Bifidobacterium-containing or Bacillus-containing probiotic additives [15,16], the antibiotic chlortetracycline [14,20,21], or chemically synthesized analogues of the following plant-derived molecules: gamma-lactone, coumarin derivative, vanillin/vanylic acid, resorcinol derivative, or quercetin [16][17][18][20][21][22][23]. In the current context, these supplements affected the cecal microbiome composition and chicken productivity, providing variability in the data for the meta-analysis.…”

“…A total of 40 groups (8 control and 32 experimental) meeting these criteria were included in the study. Bark extract [14,15,19], Bifidobacterium-containing or Bacillus-containing probiotic additives [15,16], the antibiotic chlortetracycline [14,20,21], or chemically synthesized analogues of the following plant-derived molecules: gamma-lactone, coumarin derivative, vanillin/vanylic acid, resorcinol derivative, or quercetin [16][17][18][20][21][22][23]. In the current context, these supplements affected the cecal microbiome composition and chicken productivity, providing variability in the data for the meta-analysis.…”

“…Meta-analysis design to characterize the cecal microbiome of broiler chickens and its association with poultry production. Designations 1-10 indicate the experimental series described in [14][15][16][17][18][19][20][21][22][23].…”

“…In a series of our previous studies published in international [14][15][16][17][18] and local [19][20][21][22][23] scientific journals, we used standard Illumina 16S-RNA gene sequencing technology in the context of the effects of different feeding regimes on the microbiome, feed efficiency, and growth performance indices in broiler chickens. General changes in cecal microbiome composition and zootechnical parameters were shown, but the relationship between cecal microbiota composition and diversity against poultry farming productivity was constrained by a small sample size in each series.…”