The uses and future potential of microbial hollow-fibre bioreactors

Bunch, Alan William

doi:10.1016/0167-7012(88)90042-5

Cited by 32 publications

(7 citation statements)

References 24 publications

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“…Ten milliliters of starter culture was inoculated into the shell side of the reactor through an inoculation port using a sterile 20-mL syringe fitted with a hypodermic needle. Cells were delivered into the outer spongy matrix layer of the hollow-fibers by pumping PBS into the reactor from the shell side, through the walls of the hollow fibers, and into the lumen of the latter (transverse or crossflow mode; Bunch, 1988) at a flow rate of 40 mL h −1 for 1 h. The shell side of the reactor was then rinsed with PBS at a flow rate of 60 mL h −1 for 30 min to remove residual cells in this compartment. The cells were grown in situ for 24 h using growth medium (Oxoid Nutrient Broth No.…”

Section: Hollow-fiber Bioreactormentioning

confidence: 99%

“…Entrapment within hollow-fiber membranes was selected as the immobilization method of choice, offering one-step immobilization under gentle conditions, operational flexibility (gaseous and aqueous electron donors can be used), and complete containment of the cells, and therefore accumulated, reduced Tc (Bunch, 1988). A recent study compared the activities of resting cells of E. coli, pregrown anaerobically, and entrapped in membrane, foam, and gel matrices (Raihan et al, in press).…”

Section: Introductionmentioning

confidence: 99%

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Tc(VII) reduction and accumulation by immobilized cells ofEscherichia coli

Lloyd

Harding

Macaskie

1997

Biotechnol. Bioeng.

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Section: Hollow-fiber Bioreactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tc(VII) reduction and accumulation by immobilized cells ofEscherichia coli

Lloyd

Harding

Macaskie

1997

Biotechnol. Bioeng.

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“…The solution must be free of particulate materials. 63 This reduces membranes fouling caused by unwanted materials thus increasing the membrane service life. Installation of filters before the reactor units and use of non-corrosive materials prevents particulate matter.…”

Section: Fluids Characteristicsmentioning

confidence: 99%

“…50,57,60 -62 They also have high volumetric productivity, simultaneous products separation, are easily reutilized, cheap enzyme fixation, little loss of the enzyme catalytic activity, high substrate concentration, and stable lipases activity. 62 -66 However, they have several drawbacks, for instance solutions must be free of particulate materials, use of specific type of fibre, 63 diffusion resistance 64,67 and enzyme conformational changes. 68,69 Other membrane configurations having only one connection to downstream/permeate-side such as the spiralwound are not suitable for the application.…”

Section: Tsp-emr: Basic Needsmentioning

confidence: 99%

Enzymatic membrane reactors: the determining factors in two separate phase operations

Agustian¹,

Kamaruddin²,

Bhatia³

2011

J of Chemical Tech & Biotech

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A two separate phase-enzymatic membrane reactor is an attractive process since it has a large interfacial area and exchange surfaces, simultaneous reaction and separation and other benefits. Many factors influence its successful operation, and these include characteristics of the enzyme, membrane, circulating fluids and reactor operations. Although the operating conditions are the main factor, other factors must be considered before, during or after its application. At the initial stage of reactor development, the solubility of substrates and products, type of operation, membrane material and size, enzyme preparation and loading procedure, and cleanliness of the recirculated fluids should be specified. The immobilization site, reactor arrangement, dissolved or no-solvent operation, classic or emulsion operation and immobilized or suspended enzyme(s) are determined later. Some factors still need further studies. Utilization of the technology is described for use from multigram-to plant-scale capacity to process racemic and achiral compounds. The racemates were resolved primarily by kinetic resolution, but dynamic kinetic resolution has been exploited. The technology focused on hydrolytic reactions, but esterification processes were also exploited.

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“…In general, cells are cultivated in the outer shell space of the bioreactor with nutrients being supplied to the cells and (waste) products removed from the cells via the fibres. Many potential advantages of using hollow-fibre bioreactors over other systems have been identified (Bunch 1988). Several examples of their potential use with microbial cells have been mooted, including the production of lactic acid (VickRoy et al 1983) and ethanol (Inloes et al 1985).…”

Section: Introductionmentioning

confidence: 99%

The physiology of Lactococcus lactis subsp. lactis biovar. diacetylactis immobilized in hollow-fibre bioreactors: glucose, lactose and citrate metabolism at high cell densities

Smith

Schaaf

Ree

et al. 1993

Appl Microbiol Biotechnol

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Lactococcus lactis subsp, lactis biovar, diacetylactis was selected to study the physiological influences of immobilization and growth to high cell densities. Cells were cultivated on glucose or lactose medium in the presence and absence of citrate. With excess glucose the cells produced mainly lactate as the fermentation product (homofermentative) providing that not all of the substrate was consumed. The population so cultivated was exposed to extreme gradients of pH and lactate concentrations. When the glucose concentration was reduced the population showed a mixed product profile with half of the glucose being fermented to lactate, the remainder to formate, acetate, ethanol and 2,3-butanediol. Inclusion of citrate in the medium shifted the population to homofermentation, with respect to the amount of glucose or lactose consumed. The citrate was metabolized via the pyruvate-formate lyase and o~-acetolactate synthase routes. The pH of the medium was shown to strongly influence the product profile from citrate, presumably by affecting the activity of the key enzymes of pyruvate metabolism. The lactococci immobilized at high cell densities show product profiles typical of carbohydrate limitation at low dilution rates.

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The uses and future potential of microbial hollow-fibre bioreactors

Cited by 32 publications

References 24 publications

Tc(VII) reduction and accumulation by immobilized cells ofEscherichia coli

Tc(VII) reduction and accumulation by immobilized cells ofEscherichia coli

Enzymatic membrane reactors: the determining factors in two separate phase operations

The physiology of Lactococcus lactis subsp. lactis biovar. diacetylactis immobilized in hollow-fibre bioreactors: glucose, lactose and citrate metabolism at high cell densities

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