The Usefulness of Step Sectioning of Transbronchial Lung Biopsy Specimen in Diagnosing Sarcoidosis

Takayama, K.; Nagata, Nobuhiko; Miyagawa, Yosuke; Honda, Hiroshi; Shigematsu, Nobuaki

doi:10.1378/chest.102.5.1441

Cited by 11 publications

(6 citation statements)

References 6 publications

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“…This classifier is entirely new and has important molecular pathologic applications, because changes in the levels of the classifier species in tumor tissues may be detected as biomarkers. One particular powerful application of the 5-microRNA molecular classifier could be with TBLB, which has been explored as a method for early diagnosis of questionable lung infiltrations or densities (26). The diagnostic yield of TBLB is lower than that of open lung biopsy because the amount of lung tissue obtained is smaller.…”

Section: Discussionmentioning

confidence: 99%

A 5-MicroRNA Signature for Lung Squamous Cell Carcinoma Diagnosis and hsa-miR-31 for Prognosis

Tan

Qin

Zhang

et al. 2011

Clinical Cancer Research

170

100

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Purpose: Recent studies have suggested that microRNA biomarkers could be useful for stratifying lung cancer subtypes, but microRNA signatures varied between different populations. Squamous cell carcinoma (SCC) is one major subtype of lung cancer that urgently needs biomarkers to aid patient management. Here, we undertook the first comprehensive investigation on microRNA in Chinese SCC patients.Experimental Design: MicroRNA expression was measured in cancerous and noncancerous tissue pairs strictly collected from Chinese SCC patients (stages I-III), who had not been treated with chemotherapy or radiotherapy prior to surgery. The molecular targets of proposed microRNA were further examined.Results: We identified a 5-microRNA classifier (hsa-miR-210, hsa-miR-182, hsa-miR-486-5p, hsamiR-30a, and hsa-miR-140-3p) that could distinguish SCC from normal lung tissues. The classifier had an accuracy of 94.1% in a training cohort (34 patients) and 96.2% in a test cohort (26 patients). We also showed that high expression of hsa-miR-31 was associated with poor survival in these 60 SCC patients by Kaplan-Meier analysis (P ¼ 0.007), by univariate Cox analysis (P ¼ 0.011), and by multivariate Cox analysis (P ¼ 0.011). This association was independently validated in a separate cohort of 88 SCC patients (P ¼ 0.008, 0.011, and 0.003 in Kaplan-Meier analysis, univariate Cox analysis, and multivariate Cox analysis, respectively). We then determined that the tumor suppressor DICER1 is a target of hsa-miR-31. Expression of hsa-miR-31 in a human lung cancer cell line repressed DICER1 activity but not PPP2R2A or LATS2.Conclusions: Our results identified a new diagnostic microRNA classifier for SCC among Chinese patients and a new prognostic biomarker, hsa-miR-31. Clin Cancer Res; 17(21); 6802-11. Ó2011 AACR.

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Section: Discussionmentioning

confidence: 99%

A 5-MicroRNA Signature for Lung Squamous Cell Carcinoma Diagnosis and hsa-miR-31 for Prognosis

Tan

Qin

Zhang

et al. 2011

Clinical Cancer Research

170

100

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“…We have also elucidated the signifi cance of step sectioning of TBLB in the diagnosis of dif fuse lung disease [6][7][8], Recently, bronchoalveolar lavage (BAL) has been established as an important method for diagnosing and evaluating diffuse lung disease, especially interstitial lung disease (ILD) [9,10] cyte content as one of markers reflecting disease activity of ILD [11][12][13][14][15]. In our clinical practice, histopathological features of TBLB, especially mononuclear cell infiltration in the interalveolar septum, have also been used to esti mate the activity of ILD.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Alveolar Septal Inflammation to Bronchoalveolar Lavage in Interstitial Lung Diseases

Nagata

Takayama²,

Nikaido³

et al. 1996

Respiration

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We analyzed transbronchial lung biopsy (TBLB) specimens by morphometry using a computer-aided graphic analyzer to quantify the severity of alveolar septal inflammation and investigated the correlation between the severity of inflammation and bronchoalveolar lavage fluid (BALF) lymphocyte content in various types of interstitial lung disease (ILD). Bronchoalveolar lavage was performed in the right S⁴ or S⁵ with 3 × 50 ml of sterile saline in 55 hospitalized patients with ILD between 1989 and 1993. Fluoroscopy-guided TBLB was then performed in the right S¹, S², S⁸ and S⁹. The severity of alveolar septal inflammation in TBLB specimens was quantified and expressed as the linear density of mononuclear cells infiltrating the alveolar septum. The severity of alveolar septal inflammation was not correlated with BALF lymphocyte content in the overall study populations (n = 55, p = 0.55), but was significantly correlated with BALF lymphocyte content in patients with hypersensitivity pneumonitis (n = 7, p = 0.021). No positive correlation was observed in patients with other types of ILD such as sarcoidosis (n = 17, p = 0.71), interstitial pneumonitis/fibrosis (n = 22, p = 0.98) or pneumoconiosis (n = 9, p = 0.48).

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“…All 16 biopsies received a minimum of 10 serial sections and in 3 biopsy samples, granulomas were only detected in deeper sections. Takayama et al [17] found a similar result in transbronchial biopsy where step sectioning of specimens increased the yield from 38% to 47% in patients with stage I lung disease and from 57% to 82% in stage II disease.…”

Section: Discussionmentioning

confidence: 77%

“…The recovery of granulomas with conjunctival biopsy using careful sectioning technique approaches the yield obtained from other tissue sites [16,17], especially when conjunctival pathology such as nodules, follicles, or fibrous scarring is present [4,12,18]. No false positives have been reported in previous studies [18,19].…”

Section: Discussionmentioning

confidence: 99%

Directed conjunctival biopsy and impact of histologic sectioning methodology on the diagnosis of ocular sarcoidosis

Bui

García-González

Patel

et al. 2014

J Ophthal Inflamm Infect

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Background: Sarcoidosis is an idiopathic, multi-system, granulomatous disease with well-described ocular manifestations. However, other uveitic etiologies can manifest in a similar fashion, and ocular disease may precede systemic manifestations. Definitive diagnosis requires histologic confirmation of non-caseating granulomatous inflammation. This study reports the diagnostic yield of directed biopsy of conjunctival follicles in patients with uveitis suspected to be secondary to sarcoidosis, and compares an institutional standard tissue sectioning method to a multi-plane technique. Results: A retrospective analysis was performed of all patients who underwent directed conjunctival biopsy for suspected ocular sarcoidosis. A total of eight patients were identified; all were females. Directed conjunctival biopsy was positive in three of seven patients using standard histologic processing method, a yield of 43%. Using the multi-plane technique increased the cumulative yield to 63%.

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The Usefulness of Step Sectioning of Transbronchial Lung Biopsy Specimen in Diagnosing Sarcoidosis

Cited by 11 publications

References 6 publications

A 5-MicroRNA Signature for Lung Squamous Cell Carcinoma Diagnosis and hsa-miR-31 for Prognosis

A 5-MicroRNA Signature for Lung Squamous Cell Carcinoma Diagnosis and hsa-miR-31 for Prognosis

Comparison of Alveolar Septal Inflammation to Bronchoalveolar Lavage in Interstitial Lung Diseases

Directed conjunctival biopsy and impact of histologic sectioning methodology on the diagnosis of ocular sarcoidosis

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