The usefulness of Neisseria gonorrhoeae strain typing by Pulse-Field Gel Electrophoresis (PFGE) and DNA detection as the forensic evidence in child sexual abuse cases: a case series

Sathirareuangchai, Sakda; Phuangphung, Peerayuht; Leelaporn, Amornrut; Boon-yasidhi, Vitharon

doi:10.1007/s00414-014-1007-z

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…In one modern example, researchers have examined commercial honey products for the presence of Clostridium botulinum as a vector for cases of induced poisoning from botulism [4]. More recently, Neisseria gonorrhoeae infections in children have been considered as evidence in sexual assault cases [5]. As such, the application of microbes in forensic applications is not a new concept.…”

Section: Introductionmentioning

confidence: 99%

The Application of The Skin Virome for Human Identification

Graham

Clarke

Fernando

et al. 2021

Preprint

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The use of skin virome for human identification purposes offers a unique approach to instances where a viable and statistically relevant human DNA profile is unavailable. The human skin virome may act as an alternative DNA profile and/or an additional form of probative genetic material. To date, no study has attempted to investigate the human virome over a time series across various physical locations of the body to identify its potential as a tool for human identification. For this study, we set out to evaluate the stability, diversity, and individualization of the human skin virome. An additional goal was to identify viral signatures that can be used in conjunction with traditional forensic STR loci. In order to accomplish this, human virome metagenomes were collected and sequenced from 42 individuals at three anatomical locations (left hand, right hand, and scalp) across multiple collections periods over a 6-month window of time. Assembly dependent and independent bioinformatic approaches were employed, along with a database-based assessment, which resulted in three sets of stable putative viral markers. In total, with the three sets combined, 59 viral species and uncharacterized viral genome assemblies were identified as being significantly stable (P=5.3x10-15). Viral diversity, based on presence or absence, is significantly different across subjects (P<0.001). Here we demonstrate that not only is the human virome applicable to be used for human identification, but we have identified many viral signatures that can be used for forensic applications, thus providing a foundation to the novel field of forensic virology.

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Section: Introductionmentioning

confidence: 99%

The Application of The Skin Virome for Human Identification

Graham

Clarke

Fernando

et al. 2021

Preprint

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“…The constantly evolving NG-MAST loci are known to undergo changes to their porB and tbpB alleles over relatively short periods of time which would explain their distribution over a wide range of PT. Although PFGE exhibited higher discriminatory power compared to NG-MAST, based on this study, use of both methods is likely to yield more definitive results in medico-legal investigations [ 7 , 8 ]. Further comparisons of the performance of these two methods over a larger population would possibly yield more conclusive results.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of Neisseria gonorrhoeae using multi-antigen sequence typing and pulse-field gel electrophoresis in highly endemic Western Australian populations

et al. 2015

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BackgroundThe remote and indigenous populations of Western Australia (WA) have one of the highest notification rates of gonorrhoea in the world. Despite this, the low rate of antimicrobial resistance in Neisseria gonorrhoeae from these regions permits the use of amoxycillin as empirical therapy. We describe the first molecular epidemiological study of gonococci isolated from this population using two different typing platforms.MethodsPulse-field gel electrophoresis (PFGE), Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST) and antimicrobial susceptibility tests were performed on 128 consecutive N. gonorrhoeae isolates cultured between January 2011 and December 2013. To highlight clusters isolates were evaluated based on their tbpB sequence types.ResultsNo predominant NG-MAST or PFGE types were found. A total of 67 distinct PFGE pulsotypes were identified amongst the 128 isolates in this study with 20 PFGE pulsotypes representing 78 isolates. A total of 59 NG-MAST sequence types were found, represented by 45 porB alleles and 28 tbpB alleles with 13 tbpB genomogroups from 45 NG-MAST sequence types. TbpB genomogroup 29, represented by 45 isolates, was by far the most common genomogroup overall.ConclusionsResults from this study suggest that gonococcal epidemiology in WA is quite different between remote regions and major population centres and, in some cases, geographically restricted. It is likely that isolates originating from endemic regions of WA mostly represent independent, small sexual networks with an infrequent interchange between other communities and regions. Given the high rate of antimicrobial resistance elsewhere in Australia, ongoing surveillance is essential to ensure the enduring efficacy of amoxycillin empiric use in the remote regions of WA.

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“…Ayrıca kurbanda saldırı/istismar öncesi negatif olan sonuçların saldırı sonrası pozitif olması durumunda, CYBH ile saldırı ya da istismarın illiyetinin belirlenmesi açısından şüpheli veya saldırganın muayeneleri de özel önem taşımaktadır. N. gonorrhoeae suşlarının moleküler yöntemlerle tiplendirilmesiyle saldırganın kimliğinin saptandığı olgular da bildirilmiştir (53,54).…”

Section: şüPheli Veya Sanığın Muayenesi Sırasında Dikkat Edileceklerunclassified

Cinsel Saldırı ve İstismar Olgularında Cinsel Yolla Bulaşan Hastalıkların Değerlendirilmesi

Çağlayan¹,

Kaya²,

Aktaş³

2020

Bull Leg Med

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Cinsel saldırı ve cinsel istismar olgularının fiziksel ve ruhsal travma ile birlikte cinsel yolla bulaşan hastalıklar (CYBH) açısından da değerlendirilmesi önemlidir. Bu olgularda muayene genellikle travma bulgularının tespiti ve saldırganın kimliğinin tespitine yönelik örnek alımı ile sınırlı olmaktadır. Cinsel yolla bulaşan Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Hepatit B virüsü, Human immunodeficiency virüs ve sifiliz gibi çok sayıda etken mevcut olmakla birlikte ülkemizde bu etkenlere yönelik tarama testleri ve profilaksi uygulamaları ile ilgili standardize edilmiş bir rehber bulunmamaktadır. Bu nedenle, tüm olgularda bulaş riski göz önünde bulundurulmalı, gereklilik halinde tedavi ve profilaksi uygulamaları açısından değerlendirme yapılmalıdır. Ayrıca saldırgana ulaşılabildiği durumlarda gerek mağdurun CYBH için tıbbi bakım ihtiyacının belirlenebilmesi gerekse saldırganın yargılanma süreci ve illiyet bağının kurulabilmesi için bu kişilerin de muayenesi ve tetkikleri yapılmalıdır. Bu derlemede, cinsel saldırı ve cinsel istismar mağdurlarında görülebilecek cinsel yolla bulaşan hastalıkların değerlendirilmesi ve saldırganın muayenesi hususlarının, literatür bilgileri eşliğinde, adli-tıbbi yönden incelenerek sağlık çalışanlarının bu konuya dikkatlerini çekmek amaçlandı.

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The usefulness of Neisseria gonorrhoeae strain typing by Pulse-Field Gel Electrophoresis (PFGE) and DNA detection as the forensic evidence in child sexual abuse cases: a case series

Cited by 7 publications

References 20 publications

The Application of The Skin Virome for Human Identification

The Application of The Skin Virome for Human Identification

Molecular epidemiology of Neisseria gonorrhoeae using multi-antigen sequence typing and pulse-field gel electrophoresis in highly endemic Western Australian populations

Cinsel Saldırı ve İstismar Olgularında Cinsel Yolla Bulaşan Hastalıkların Değerlendirilmesi

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