The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans

Krzyściak, Wirginia; Pluskwa, Katarzyna K; Piątkowski, Jakub; Krzyściak, Paweł; Jurczak, Anna; Kościelniak, Dorota; Skalniak, Anna

doi:10.1186/1471-2180-14-194

Cited by 14 publications

(19 citation statements)

References 38 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S. sanguinis (38.46%), L. rhamnosus (30.77%), S. mitis (19.23%) and S. mutans (11.54%) ( Figure 3) are consistent with the results of Aas et al [35] and van Hout et al [36], who demonstrated that 10% of children and teenagers (aged 2-21) did not have S. mutans at all. This also corroborates our earlier study, in which S. mutans constituted less than 9% in this group [23].…”

Section: Discussionsupporting

confidence: 93%

“…The second method for biotype determination consisted of the use of an unattended (without a priori available knowledge) statistical data analysis. In this method, data were divided into groups (clusters), so that each group was as homogenous as possible and, at the same time, clusters differed from one another (strains from different groups had the lowest possible number of common characters) [23].…”

Section: Bacterial Identificationmentioning

confidence: 99%

See 1 more Smart Citation

The role of the saliva antioxidant barrier to reactive oxygen species with regard to caries development

et al. 2017

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Bacterial Identificationmentioning

confidence: 99%

The role of the saliva antioxidant barrier to reactive oxygen species with regard to caries development

et al. 2017

View full text Add to dashboard Cite

show abstract

“…De acuerdo a lo que manifiesta Krzysciak y colaboradores [18] muy seguramente tener información y control de los hábitos higiénicos que llevan los niños, permitirá entender mejor el papel de los biotipos de S. mutans y su relación en la patogenicidad en niños con y sin caries dental.…”

Section: Discussionunclassified

“…La prueba api-ZYM (bioMérieux Marcy-létoile, France) no es la prueba de "oro" para la caracterización biotípica de S. mutans. Existe otra prueba, Sistema STREPTOtest (Lachema, Pliva) [18] que también se ha utilizado con este fin. La idea de utilizar estos sistemas es poder determinar algunas diferencias que presenta esta bacteria en su sistema enzimático.…”

Section: Discussionunclassified

Biotipos y susceptibilidad antimicrobiana de S. mutans en niños con y sin caries dental

Gamboa

García

Lamby

et al. 2016

Rev. Colomb. Cienc. Quim. Farm.

View full text Add to dashboard Cite

Objetivos: investigar los biotipos de S. mutans aislados de niños con y sin caries dentaly determinar su susceptibilidad antimicrobiana. Métodos: este estudio observacionaldescriptivo incluyó 206 niños entre los 3 y 5 años de dos preescolares en Bogotá, D.C., Colombia. Después de su aislamiento en agar Mitis Salivarius Bacitracina, lascepas S. mutans se identificaron por pruebas bioquímicas y se biotipificaron por elsistema api-ZYM. En todos los aislamientos se determinó por el método de diluciónenvagar la concentración inhibitoria mínima (CIM) de Penicilina, Amoxicilina, Cefazolina, Eritromicina, Clindamicina, Imipenem, Vancomicina y Teicoplanina.Resultados: Se encontró S. mutans en 30 de los 79 niños sin caries dental (38%) y en56 de los 127 niños con caries (44,1%). Los niños con caries presentaron un recuentosuperior de S. mutans en comparación con los niños sin caries (p < 0,05). En totalse identificaron 121 cepas de S. mutans en los 86 niños: 43 cepas en los 30 niños sincaries dental y 78 en los 56 niños con caries dental. Las 121 cepas fueron agrupadasen 38 biotipos: 24 en el grupo de caries y 14 en el grupo sin caries. En el grupo decaries los biotipos más frecuentes fueron el XV, XI, XII y XVII, respectivamente,con 26, 12, 11 y 4 cepas y en el grupo sin caries los biotipos más frecuentes fueronel XXVI, XX y XXXVI, respectivamente, con 14, 8 y 7 cepas. Todos los biotipos

show abstract

“…The previous studies suggested that these bacteria were significantly related to both caries in small children and the physiological flora of the oral cavity [181,182]. This fact is not confirmed by recent study Krzyściak et al's [183] in which, apart from a detailed description of S. mutans, constituting 24% of natural physiological flora of children without symptoms of the caries disease and 77% of cariogenic flora in children with early childhood caries (ECC), presence of S. parasanguinis constituted only 2%. The presence of S. mutans bacteria onto the teeth surfaces is usually related to poor hygiene and diet.…”

Section: Role Of Oral Microbiome In the Formation Of Dental Plaquementioning

confidence: 84%

The Role of Human Oral Microbiome in Dental Biofilm Formation

Krzyściak¹,

Jurczak²,

Piątkowski³

2016

Microbial Biofilms - Importance and Applications

Self Cite

View full text Add to dashboard Cite

Each surface of the human body, which stays in contact with the external environment, is covered by a layer of microorganisms. This layer-the human microbiomeis characterized by a high diversity of species and huge number of cells. Its name was proposed by Joshua Lederberg at the turn of the twentieth and twenty-first centuries and was originally referred to as a group of microorganisms colonizing a certain habitat. Currently, the term also defines a set of genomes of all organisms inhabiting a particular niche. Since the human microbiota affects many aspects of human health, it has become the subject of different studies. The use of sequencing methods enabled to obtain genetic material derived directly from the human environment with simultaneous explanation of mutual relationships between microorganisms inhabiting different ecological niches of human organism (i.e., commensal, symbiotic, and pathogenic microorganisms). It is hard to determine the amount of microbiota inhabiting human oral cavity because microbiota represents distinct anatomically limited ecological niches; for example, microbiota of tongue surface, cheek, teeth, palate, gingiva, and periodontal pocket. Apart from anatomical structure, other factors determine different composition of particular oral cavity microbiota. These factors are various qualities of saliva-a natural protective barrier ensuring maintenance of healthy condition of the oral cavity-and habits of diet and hygiene. Generally, bacteria are passively transported by flowing saliva toward teeth surfaces. In turn, the pioneering microorganisms initiating changes in the environment of oral cavity through the production and secretion of products of their metabolism induce mutual microbiota-biofilm interactions. The formation of biofilm of the plaque is a complex and rapidly evolving process in which several stages of development can be distinguished arbitrarily: (i) reversible binding of bacteria to solid surfaces, (ii) production of exopolysaccharide matrix, (iii) irreversible binding to the surface, (iv) maturation of biofilm structure, (v) disintegration and dispersion of an organized structure, and (vi) the formation of new habitats. An oral microbiome

show abstract

The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans

Cited by 14 publications

References 38 publications

The role of the saliva antioxidant barrier to reactive oxygen species with regard to caries development

The role of the saliva antioxidant barrier to reactive oxygen species with regard to caries development

Biotipos y susceptibilidad antimicrobiana de S. mutans en niños con y sin caries dental

The Role of Human Oral Microbiome in Dental Biofilm Formation

Contact Info

Product

Resources

About