The Use of Zebrafish Xenotransplant Assays to Analyze the Role of lncRNAs in Breast Cancer

Zampedri, Cecilia; Martinez-Flores, Williams Arony; Meléndez-Zajgla, Jorge

doi:10.3389/fonc.2021.687594

Cited by 6 publications

(7 citation statements)

References 157 publications

(154 reference statements)

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“…Zebrafish cell xenotransplantation studies have the advantage of maintaining the effects of the microenvironment in cell communication and cancer progression, even when there are inter species differences. 23 When cancer cells are implanted, many different zebrafish cells are recruited to the tumor site following tumor instructions. 24,25 Zebrafish xenotransplantation of cancer cells enabled the discovery of a new mechanism of metastatic niche formation, and the roles of macrophages in this process were described.…”

Section: Senescence and Cancer Microenvironmentmentioning

confidence: 99%

Pez cebra: modelado de senescencia en el contexto de la enfermedad y la regeneración

Carrillo-Rosas¹,

Ríos-Pérez²,

Zampedri³

2022

Investigación en Discapacidad

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Cellular senescence is a natural biological process characterized by a permanent and irreversible state of cellular arrest, mitochondrial alteration, and secretion of senescence-associated phenotype (SASP) components. Several factors can induce senescence, including but not limited to DNA damage, oxidative stress, and neuroinflammation, these factors have also been linked to several disorders such as Alzheimer's, Parkinson's, cancer, among others. The increased presence of senescent cells among different diseases suggests the importance of senescence in the pathophysiology of a great number of disorders, thus the need for different models that could help deepen our understanding of the molecular mechanisms of senescence, identify possible targets for therapeutic interventions, and arising challenges. In addition to in vitro models, most senescent research has come from classical model species, i.e., mouse (Mus musculus) and rat (Rattus norvegicus). However, senescence is highly conserved; different studies have shown that senescent cells seem to accumulate in all vertebrate organisms and that several associated genes show similar expression patterns, opening the door to new vertebrate models. The zebrafish has become a strong emerging model for different diseases, such as cancer, inflammation, neurodegeneration, among others; it shares multiple advantages with classical models, such as well-established genome editing tools and a fully sequenced genome. Additionally, zebrafish exhibit multiple advantages, including high fecundity for robust statistical analysis, external fertilization, and optical transparency that enables powerful imaging capabilities and makes it a versatile model for experimental manipulation and structural visualization. Here we present the zebrafish as a model that can contribute significantly to our understanding of the processes involved in senescence and age-related diseases. ResumenLa senescencia celular es un proceso biológico natural caracterizado por un estado permanente e irreversible de arresto celular, alteraciones mitocondriales y secreción de componentes del fenotipo asociado a la senescencia (SASP). Varios factores pueden inducir la senescencia, incluidos el daño al ADN, estrés oxidante y neuroinflamación; estos factores también se han relacionado con varios trastornos como el Alzheimer, el Parkinson, el cáncer, entre otros. La mayor presencia de células senescentes entre diferentes enfermedades sugiere la importancia de la senescencia en la fisiopatología de un gran número de trastornos, por lo tanto, la necesidad de diferentes modelos que podrían ayudar a profundizar nuestra comprensión de los mecanismos moleculares de la senescencia, identificar posibles objetivos para intervenciones terapéuticas y los desafíos que surgen. Además de los modelos in vitro, la mayoría de las investigaciones senescentes provienen de especies modelo clásicas, es decir, ratón (Mus musculus) y rata (Rattus norvegicus). Sin embargo, la senescencia está muy conservada;

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Section: Senescence and Cancer Microenvironmentmentioning

confidence: 99%

Pez cebra: modelado de senescencia en el contexto de la enfermedad y la regeneración

Carrillo-Rosas¹,

Ríos-Pérez²,

Zampedri³

2022

Investigación en Discapacidad

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“…The generation of tumor xenograft in zebrafish embryos and larvae, or zebrafish avatar, represents an opportunity to study tumor growth, angiogenesis, cell invasion and metastatic dissemination, the interaction between tumor and host in vivo avoiding immunogenic rejection, representing a promising platform for the translational research and personalized therapies [49]. This approach could be applied using both breast cancer cell lines and a patient's biopsy to perform drug testing.…”

Section: Clinically Translatable Promises: Next 10 Yearsmentioning

confidence: 99%

Precision Medicine: Technological Impact into Breast Cancer Diagnosis, Treatment and Decision Making

Tilli

2021

JPM

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Breast cancer is the most common cancer in women, impacting 2.1 million women each year. The number of publications on BC is much higher than for any other type of tumor, as well as the number of clinical trials. One of the consequences of all this information is reflected in the number of approved drugs. This review aims to discuss the impact of technological advances in the diagnosis, treatment and decision making of breast cancer and the prospects for the next 10 years. Currently, the literature has described personalized medicine, but what will the treatment be called for in the coming years?

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“…We have further evaluated the potential of our designed peptide-functionalized nanorods in a zebrafish xenotransplant model for in vivo monitoring of prevention of micrometastasis. This model has helped us to visualize and understand the tumor cell behavior in real time in a transparent animal model …”

Section: Introductionmentioning

confidence: 99%

“…This model has helped us to visualize and understand the tumor cell behavior in real time in a transparent animal model. 30 ■ EXPERIMENTAL SECTION Materials. We procured Fmoc-protected amino acids and resins from NovaBiochem and GL Biochem.…”

Section: ■ Introductionmentioning

confidence: 99%

Harnessing Peptide-Functionalized Multivalent Gold Nanorods for Promoting Enhanced Gene Silencing and Managing Breast Cancer Metastasis

Chakraborty

Biswas

Mishra

et al. 2023

ACS Appl. Bio Mater.

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Small interfering RNA (siRNA) has become the cornerstone against undruggable targets and for managing metastatic breast cancer. However, an effective gene silencing approach is faced with a major challenge due to the delivery problem. In our present study, we have demonstrated efficient siRNA delivery, superior gene silencing, and inhibition of metastasis in triple-negative breast cancer cells (MDA-MB-231) using rod-shaped (aspect ratio: 4) multivalent peptide-functionalized gold nanoparticles and compared them to monovalent free peptide doses. Multivalency is a new concept in biology, and tuning the physical parameters of multivalent nanoparticles can enhance gene silencing and antitumor efficacy. We explored the effect of the multivalency of shape- and size-dependent peptide-functionalized gold nanoparticles in siRNA delivery. Our study demonstrates that peptide functionalization leads to reduced toxicity of the nanoparticles. Such designed peptide-functionalized nanorods also demonstrate antimetastatic efficacy in Notch1-silenced cells by preventing EMT progression in vitro. We have shown siRNA delivery in the hard-to-transfect primary cell line HUVEC and also demonstrated that the Notch1-silenced MDA-MB-231 cell line has failed to form nanobridge-mediated foci with the HUVEC in the co-culture of HUVEC and MDA-MB-231, which promote metastasis. This antimetastatic effect is further checked in a xenotransplant in vivo zebrafish model. In vivo studies also suggest that our designed nanoparticles mediated inhibition of micrometastasis due to silencing of the Notch1 gene. The outcome of our study highlights that the structure–activity relationship of multifunctional nanoparticles can be harnessed to modulate their biological activity.

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The Use of Zebrafish Xenotransplant Assays to Analyze the Role of lncRNAs in Breast Cancer

Cited by 6 publications

References 157 publications

Pez cebra: modelado de senescencia en el contexto de la enfermedad y la regeneración

Pez cebra: modelado de senescencia en el contexto de la enfermedad y la regeneración

Precision Medicine: Technological Impact into Breast Cancer Diagnosis, Treatment and Decision Making

Harnessing Peptide-Functionalized Multivalent Gold Nanorods for Promoting Enhanced Gene Silencing and Managing Breast Cancer Metastasis

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