The use of whole-genome sequencing for molecular epidemiology and antimicrobial surveillance: identifying the role of IncX3 plasmids and the spread of<i>bla</i><sub>NDM-4</sub>-like genes in the Enterobacteriaceae

g Enterobacteriaceae with bla NDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring bla NDM-7 . To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with bla NDM-7 .

Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Chen

Peirano

Lynch

et al. 2016

“…Additionally, NDM-producing Enterobacteriaceae have been reported in Australia (27,28). Limited studies have described the plasmid features and genetic contexts of NDM producers from Australia (29)(30)(31). Here, we analyze the bla NDM genetic contexts of 12 NDM-producing Enterobacteriaceae isolates from Australia between 2012 and 2014 to provide insights into their likely acquisition.…”

mentioning

confidence: 99%

Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their bla _NDM Genetic Contexts

Wailan

Paterson

Kennedy

et al. 2016

e bla NDM has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. The genetic context of bla NDM is heterogeneous. In this study, we genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014: Escherichia coli (n ‫؍‬ 6), Klebsiella pneumoniae (n ‫؍‬ 3), Enterobacter cloacae (n ‫؍‬ 2) and Providencia rettgeri (n ‫؍‬ 1). We describe their bla NDM genetic contexts within Tn125, providing insights into the acquisition of bla NDM into Enterobacteriaceae. IncFII-type (n ‫؍‬ 7) and IncX3 (n ‫؍‬ 4) plasmids were the most common plasmid types found. The IncHI1B (n ‫؍‬ 1) plasmid was also identified. Five different bla NDM genetic contexts were identified, indicating four particular plasmids with specific bla NDM genetic contexts (NGCs), three of which were IncFII plasmids (FII-A to -C). Of note, the bla NDM genetic context of P. rettgeri was not conjugative. Epidemiological links between our NDM-producing Enterobacteriaceae were established by their acquisition of these five particular plasmid types. The combination of different molecular and genetic characterization methods allowed us to provide insight into the spread of plasmids transmitting bla NDM .

“…In GenBank deposit AB898038 (19), an IS6 family transposase truncates the ISAba125 and an unknown transposase is present 2.367 kb downstream from the bla NDM-3 gene. In K. pneumoniae plasmid pJEG027 (20), an IS5 family transposase truncates the ISAba125 and IS26 is found 2.189 kb downstream from the bla NDM-4 gene. A similar genetic structure is present in GenBank deposits KP826705 (unpublished) and KP178355 (21), containing, respectively, the bla NDM-7 and bla NDM-5 genes.…”

mentioning

confidence: 99%

Characterization of Tn 3000 , a Transposon Responsible for bla _NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

Campos

Silva

Santos³

et al. 2015

kIn Enterobacteriaceae, the bla NDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the bla NDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFII K and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the bla NDM-1 gene may be transmitted by Tn3000 in different parts of the world.