The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study

Al-Fotawei, Randa; Naudi, Kurt; Lappin, David F.; Barbenel, J.C.; Silvio, L. Di; Hunter, Keith D.; McMahon, Jeremy; Ayoub, Ashraf

doi:10.1016/j.jcms.2013.12.006

Cited by 26 publications

(13 citation statements)

References 37 publications

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“…The harvesting of vascularised bone graft is associated with well documented morbidities [2]. Bone bioengineering using biomaterial, bioactive molecules and autogenous stem cells have been studied extensively and variable rates of success are reported [3][4][5]. Other studies have investigated various ways to induce angiogensesis and arteriogenesis which are essential for the bone regeneration process [6,7].…”

Section: Introductionmentioning

confidence: 99%

Radiological assessment of bioengineered bone in a muscle flap for reconstruction of a critical-size mandibular defect

Al-Fotawei

Odell

Naudi

et al. 2015

International Journal of Oral and Maxillofacial Surgery

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This study presents a comprehensive radiographic evaluation of bone regeneration within a pedicled muscle flap for the reconstruction of critical size mandibular defect. The surgical defect (20 mm615 mm) was created in the mandible of ten experimental rabbits. The masseter muscle was adapted to fill the surgical defect, a combination of calcium sulphate/ hydroxyapatite cement (CERAMENT TM |SPINE SUPPORT), BMP-7 and rabbit mesenchymal stromal cells (rMSCs) was injected inside the muscle tissue. Radiographic assessment was carried out on the day of surgery and at 4, 8, and 12 weeks postoperatively. At 12 weeks, the animals were sacrificed and cone beam computerized tomography (CBCT) scanning and micro-computed tomography (m-CT) were carried out. Clinically, a clear layer of bone tissue was identified closely adherent to the border of the surgical defect. Sporadic radio-opaque areas within the surgical defect were detected radiographically. In comparison with the opposite non operated control side, the estimated quantitative scoring of the radio-opacity was 46.6% 615, the mean volume of the radio-opaque areas was 63.4% 620. Areas of a bone density higher than that of the mandibular bone (+35% 625%) were detected at the borders of the surgical defect. The micro-CT analysis revealed thinner trabeculae of the regenerated bone with a more condensed trabecular pattern than the surrounding native bone. These findings suggest a rapid deposition rate of the mineralised tissue and an active remodelling process of the newly regenerated bone within the muscle flap. The novel surgical model of this study has potential clinical application; the assessment of bone regeneration using the presented radiolographic protocol is descriptive and comprehensive. The findings of this research confirm the remarkable potential of local muscle flaps as local bioreactors to induce bone formation for reconstruction of maxillofacial bony defects.

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Section: Introductionmentioning

confidence: 99%

Radiological assessment of bioengineered bone in a muscle flap for reconstruction of a critical-size mandibular defect

Al-Fotawei

Odell

Naudi

et al. 2015

International Journal of Oral and Maxillofacial Surgery

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate craniofacial tissue engineering 49‐72,74‐77,79,81‐84,145‐197 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…A diferencia de otros modelos experimentales de grandes defectos en mandíbula de conejos, en los cuales los segmentos remanentes son estabilizados de manera trans mandibular (Alfotawei;Naudi et al, 2012), no entrega una realidad cercana a lo que se realiza en cirugía reconstructiva tumoral.…”

Section: Discussionunclassified

“…La utilización de modelos experimentales es aceptada para el estudio de la bioingeniería ósea; la utilización de conejos se debe principalmente por ser éstos biológicamente similar al humano y ser de fácil mantención y almacenaje debido a su tamaño reducido (Lu & Rabie, 2002;Alfotawei et al, 2014). Sin embargo, los estudios experimentales generalmente se realizan en modelos de defectos pequeños en calota (Cantín et al, 2015;Rodriguez et al, 2016) o modelos de defectos mayores en animales de mayor tamaño como perros, cabras o primates (Yamauchi et al, 2008 al., 2016).…”

Section: Introductionunclassified

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

et al. 2017

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Morphol., 35(3):1185Morphol., 35(3): -1190Morphol., 35(3): , 2017. RESUMEN:El propósito de este estudio fue de evaluar la técnica quirúrgica propuesta para estudios de resección y reconstrucción mandibular en modelos experimentales en conejos. Se utilizaron 7 conejos neozelandeses (Orictolagus cuniculus), en los cuales se realizó la resección del cuerpo mandibular izquierdo, una vez asegurada la movilidad de los segmentos, se reconstruyó con placa de osteosíntesis de titanio de 1,5 mm y tornillos monocorticales de 5 mm, en la zona resecada se fijó un bloque de beta fosfato tricálcico y estabilizado con tornbillo de 1,2 mm. Se sacrificaron los animales a los 2 meses. Todos los animales sobrevivieron al procedimiento y se pudo llevar a cabo la resección y reconstrucción en todos ellos. No se presentaron signos ni síntomas de infección en el sitio quirúrgico mientras duró el experimento. El tiempo promedio de cirugía fue de 68 minutos. El peso promedio de los animales fue de 3925 g, la pérdida promedio de peso fue de 2,03 %. No se realizó eutanasia de ningún animal por motivos de sufrimiento. Al examen macroscópico de las muestras extraídas se observó la presencia de todos los elementos de osteosíntesis en posición, así como continuidad de la estructura ósea casi en su totalidad en la superficie . La utilización de conejos en modelos de reconstrucción maxilofacial es un modelo probado debido a la similitud de los procesos reparativos, su facilidad de manipulación y cuidados. El modelo propuesto representa una alternativa más cercana a los procesos reconstructivos en cirugía resectiva maxilofacial, debido a que somete los injertos a las fuerzas propias de la masticación y de la función orofacial.PALABRAS CLAVE: Injerto óseo; Scaffold; Ingeniería de tejidos; β β β β β-TCP.

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The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical study

Cited by 26 publications

References 37 publications

Radiological assessment of bioengineered bone in a muscle flap for reconstruction of a critical-size mandibular defect

Radiological assessment of bioengineered bone in a muscle flap for reconstruction of a critical-size mandibular defect

Tissue engineering applications in otolaryngology—The state of translation

Modelo Experimental para el Estudio de la Reconstrucción Mandibular: Opciones en Conejos Oryctolagus cuniculus

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