The use of trehalose-treated freeze-dried amniotic membrane for ocular surface reconstruction

Nakamura, Takahiro; Sekiyama, Eiichi; Takaoka, Maho; Bentley, Adam J.; Yokoi, Norihiko; Fullwood, Nigel J.; Kinoshita, Shigeru

doi:10.1016/j.biomaterials.2008.05.023

Cited by 54 publications

(46 citation statements)

References 21 publications

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“…Following our previous protocol (45), whole eyes were examined by transmission electron microscopy (TEM). Specimens were fixed in 2.5% glutaraldehyde in 0.1 M PBS, washed 3 times for 15 minutes in PBS, and post-fixed for 2 hours in 2% aqueous osmium tetroxide.…”

Section: Figurementioning

confidence: 99%

“…Mouse corneal epithelial cells were cultured according to our previously reported system (43)(44)(45)(46). Corneal epithelial explants, including the corneal limbus (n = 3), were put onto denuded amniotic membrane substrate spread on the bottom of culture inserts and cocultured for 14 days with mitomycin C-inactivated 3T3 fibroblasts (2 × 10 4 cells/cm 2 ).…”

Section: Figurementioning

confidence: 99%

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LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis

Nakamura¹,

Hamuro²,

Takaishi³

et al. 2013

J. Clin. Invest.

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Corneal integrity and transparency are indispensable for good vision. Cornea homeostasis is entirely dependent upon corneal stem cells, which are required for complex wound-healing processes that restore corneal integrity following epithelial damage. Here, we found that leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is highly expressed in the human holoclone-type corneal epithelial stem cell population and sporadically expressed in the basal cells of ocular-surface epithelium. In murine models, LRIG1 regulated corneal epithelial cell fate during wound repair. Deletion of Lrig1 resulted in impaired stem cell recruitment following injury and promoted a cell-fate switch from transparent epithelium to keratinized skin-like epidermis, which led to corneal blindness. In addition, we determined that LRIG1 is a negative regulator of the STAT3-dependent inflammatory pathway. Inhibition of STAT3 in corneas of Lrig1 -/-mice rescued pathological phenotypes and prevented corneal opacity. Additionally, transgenic mice that expressed a constitutively active form of STAT3 in the corneal epithelium had abnormal features, including corneal plaques and neovascularization similar to that found in Lrig1 -/-mice. Bone marrow chimera experiments indicated that LRIG1 also coordinates the function of bone marrow-derived inflammatory cells. Together, our data indicate that LRIG1 orchestrates corneal-tissue transparency and cell fate during repair, and identify LRIG1 as a key regulator of tissue homeostasis.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis

Nakamura¹,

Hamuro²,

Takaishi³

et al. 2013

J. Clin. Invest.

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“…[11][12][13] The AM can be used as a scaffold either with the amniotic epithelium (epithelial AM [eAM]) or without it (denuded AM [dAM]). 14 In addition, the AM stromal side (AM stroma [sAM]) also has proven to be a promising scaffold.…”

Section: Introductionmentioning

confidence: 99%

Amniotic Membrane Scaffolds Enable the Development of Tissue-Engineered Urothelium with Molecular and Ultrastructural Properties Comparable to that of Native Urothelium

Jerman

Veranič

Kreft

2014

Tissue Engineering Part C: Methods

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The amniotic membrane (AM) is a naturally derived biomaterial that possesses biological and mechanical properties of great importance for tissue engineering. The aim of our study was to determine whether the AM enables the formation of a normal urinary bladder epithelium-urothelium-and to reveal any differences in the urothelial cell (UC) growth and differentiation when using different AM scaffolds. Cryopreserved human AM was used as a scaffold in three different ways. Normal porcine UCs were seeded on the AM epithelium (eAM), denuded AM (dAM), and stromal AM (sAM) and were cultured for 3 weeks. UC growth on AM scaffolds was monitored daily. By using electron microscopy, histochemical and immunofluorescence techniques, we here provide evidence that all three AM scaffolds enable the development of the urothelium. The fastest growth and the highest differentiation of UCs were demonstrated on the sAM scaffold, which enables the development of tissue-engineered urothelium with molecular and ultrastructural properties comparable to that of the native urothelium. Most importantly, the highly differentiated urothelia on the sAM scaffolds provide important experimental models for future drug delivery studies and developing tissue engineering strategies considering that subtle differences are identified before translation to the clinical settings.

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“…Rejection rarely occurs in the amniotic membrane because it is free from vascular components [1]. Therefore, the amniotic membrane has attracted attention as a surgical biomaterial for regenerative medicine in various medical fields [2][3][4][5]. The regenerative medicine is a medical treatment that regenerates tissues damaged by disorders and external injuries using cultivated cells and tissues in vitro.…”

Section: Introductionmentioning

confidence: 99%

Tensile properties of amniotic membrane

Tanaka

Nagayama

Katayama

et al. 2010

WIT Transactions on the Built Environment

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The amniotic membrane, which is the innermost layer of the placental membrane, is a thin and transparent tissue. Rejection rarely occurs in the amniotic membrane because it is free from vascular components. Therefore, the amniotic membrane has attracted attention as a surgical biomaterial for regenerative medicine in various medical fields. However, evaluation of mechanical properties for the amniotic membrane is not standardized because of the difficulty in handling tiny amniotic membrane materials.In this study, to propose the most appropriate tensile testing method for the amniotic membrane, the influence of specimen shapes and testing jig types on the mechanical properties of the amniotic membrane is examined. Moreover, for the strain measurement of the thin film like amniotic membrane, due to the low rigidity of the specimen, it is necessary to measure the strain by a non-contact measurement method. A non-contact optical 3D deformation measuring system, which can measure strain by the image correlation method using the dotted pattern of specimen's surface, is applied for the tensile testing of the amniotic membrane. The dumbbell specimen and grip jig are shown to be suitable for tensile testing of the amniotic membrane. The tensile strength of the amniotic membrane was 6.80 ± 0.22 MPa. For strain measurement by using a non-contact optical 3D deformation measuring system, the organic solvent spray was not suitable because the ink-film was formed on the surface of the amniotic membrane. On the other hand, spray mixed with calcium carbonate and hydrophilic ink was appropriate to apply a dotted pattern on the surface of the amniotic membrane.

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The use of trehalose-treated freeze-dried amniotic membrane for ocular surface reconstruction

Cited by 54 publications

References 21 publications

LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis

LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis

Amniotic Membrane Scaffolds Enable the Development of Tissue-Engineered Urothelium with Molecular and Ultrastructural Properties Comparable to that of Native Urothelium

Tensile properties of amniotic membrane

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