The use of tissue cultures in the mass production of Heuchera‘Silver Scrolls’

Nowakowska, Karolina; Bodych, Aleksandra; Latkowska, M.; Pacholczak, A.

doi:10.22630/ahla.2020.41.1

Cited by 7 publications

(13 citation statements)

References 11 publications

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“…The cultivars mentioned above were successful in shoot regeneration by petiole in vitro culture in this study. Our ndings were consistent with previous research on shoot regeneration [5].…”

Section: Discussionsupporting

confidence: 93%

“…In the genus Heuchera, successful shoot regenerations from shoots, leaves, and petioles cultured in vitro have been reported [3,5]. However, note that the shoot explants were whole plant destructive led to mother plant loss, coupled with petioles regenerating more shoots than leaves [5]. Moreover, the direct and indirect shoot organogenesis from petiole explants has been reported in plants [6,7].…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, cutting and tissue culture are viable alternative ways to preserve distinctive phenotypic features and obtain numerous daughter plants. However, only a few varieties of Heuchera have been previously investigated in vitro, such as the cultivars Red Spangles [3], Caramel [4], Silver Scrolls [5], etc. And there are essentially no scienti c publications on its cutting propagation to date.…”

Section: Introductionmentioning

confidence: 99%

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In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Guo

Wang

et al. 2022

Preprint

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There is still a lack of understanding about the efficient mass propagation of multiple Heuchera cultivars leading to a large market gap. And there has been no empirical research on the relationship between propagation methods of cutting and in vitro. In this study, thirteen different cultivars from the genus Heuchera and Heucherella (Heuchera × Tiarella) were successfully rooted by cutting, and calluses and shoots were also successfully inducted from petiole in vitro culture. However, measured indexes varied significantly among cultivars for cutting and tissue culture. Petiole explants treated with cytokinin 6-benzylaminopurine (BA: 0.5 ~ 2.0 mg/L) plus auxin α-naphthaleneacetic acid (NAA: 0.5 mg/L) formed shoots either directly or indirectly during primary culture. And the dark treatment and 2,4-dichlorophenoxyacetic acid (2,4-D) were ineffective for shoot formation but beneficial for callus stimulation. Furthermore, we discovered a negative correlation between initiating cutting recovery and tissue culture inducting, implying that cultivars with low difficulty in cutting initiating were relatively difficult to tissue culture. Then, we developed a simple and efficient Heuchera rapid propagation protocol. That is, micropropagation through obtaining adventitious shoots directly from primary cultures, supplemented by cutting propagation. Our petiole explants system and leaf-cutting technique are expected to guide Heuchera's mass production.

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“…The cultivars mentioned above were successful in shoot regeneration by petiole in vitro culture in this study. Our ndings were consistent with previous research on shoot regeneration [5].…”

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Guo

Wang

et al. 2022

Preprint

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“…Addition of ABA to the last subculture of tobacco cuttings increased the survival rate upon transfer from in vitro to natural conditions [18]. In coral bells, 94% of plants became acclimated after being sprayed with aqueous ABA solution [9]. The ABA treatment positively affected the chlorophyll a+b content and photosynthesis parameters, improving the growth of Nicotiana tabacum L. during acclimation [19].…”

Section: Discussionmentioning

confidence: 99%

“…Water deficit and salt stress result in ABA accumulation, which leads to stomatal closure, decreasing transpiration and water loss [8]. Because of that, ABA is often used during acclimation to ex vitro conditions and its foliar application increases the survival rate of the explants [9].…”

Section: Introductionmentioning

confidence: 99%

Acclimation to Ex Vitro Conditions in Ninebark

et al. 2021

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Acclimation is the final phase of micropropagation and often decisive for its economic output. The aim of the experiments was to evaluate the effect of abscisic acid (ABA) and supplementary light on acclimation and leaf anatomy of the in vitro-rooted plants of ninebark (Physocarpus opulifolius L.). The initial material came from 8–10-week-old in vitro cultures on ½MS supplemented with 1 mg·L−1 IBA. After potting, plantlets were sprayed with ABA solutions or distilled water and were grown either under natural daylight or under supplemental sodium light at 230 μmol·m−2·s−1 between 2 and 9 p.m. All measurements and anatomical observations were done after eight weeks in the greenhouse. Supplementary lighting significantly increased the percentage of acclimatized plants, plant height and the internode number. Plant growth was also positively affected by 1 mg·L−1 ABA. During acclimation, the photosynthesis rate increased while the transpiration and stomatal conductance dropped. The assimilation pigment contents increased under supplemental lighting while ABA had no detectable effect. However, relative to water controls, ABA increased photosynthesis, reduced transpiration, and stomatal conductance in plants growing under both light conditions. Leaves from the in vitro plants were about two times thinner than those from plants growing in soil, with only a single layer of the palisade parenchyma, hence with lower proportion in relation to the spongy parenchyma. Supplementary light during acclimation increased leaf thickness but only in the water control while it decreased it in the ABA-treated plants. ABA increased the mesophyll thickness but only in plants growing under natural light. In conclusion, supplementary light and treatment with ABA enhance acclimation of micropropagated ninebark plants.

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Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

Xu,

Guo,

Wang

et al. 2023

Sci Rep

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The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.

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The use of tissue cultures in the mass production of Heuchera‘Silver Scrolls’

Cited by 7 publications

References 11 publications

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods

Acclimation to Ex Vitro Conditions in Ninebark

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

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