The use of the zebrafish model in stress research

Steenbergen, Peter J.; Richardson, Michael K.; Champagne, Danielle L.

doi:10.1016/j.pnpbp.2010.10.010

Cited by 167 publications

(162 citation statements)

References 191 publications

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“…Behaviour is now recognized as an integrative indicator of both organism stress response and physiological alterations (Champagne et al, 2010;Steenbergen et al, 2011). Two months after tagging, we compared the swimming activity of tagged fish with that of Tagged fish were grouped by sex and mass classes at tagging (100mg classes, except the largest class for female, which ranges from 651 to 850mg).…”

Section: Swimming Behaviourmentioning

confidence: 99%

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Cousin

Daouk

Péan

et al. 2012

Journal of Experimental Biology

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SUMMARYAlthough individual electronic tagging using passive integrated acoustic (PIT) tags is established, it is mainly for fish >60mm in length and is unsuitable for fish of <30mm, like zebrafish. We used radio frequency identification (RFID) microtags (1mm in diameter and 6mm in length, with a mass of ~10mg) to individually identify juvenile zebrafish (length 16-42mm, mass 138-776mg) for the first time, and studied the effects of intracoelomic implantation on fish survival and microtag loss, growth, spawning and exploratory behaviour. After 5.5months, both high survival (82%) and low microtag loss (11%) were achieved. The smallest surviving fish weighed 178mg, and success in microtag reading was 73% for the size class 350-450mg (26mm). Greater success was achieved when fish were larger at the time of tagging but no negative effects on growth were observed for any size class and some tagged fish spawned. No significant differences in behavioural responses could be detected between tagged fish and untagged controls after 2months. Overall, the results suggest that the tagging method is highly suitable for fish as small as zebrafish juveniles. We think this method will provide significant advances for researchers of the ever-growing fish model community and more generally for all small-fish users. Tagging is essential when one needs to identify fish (e.g. particular genotypes with no external cue), to run longitudinal monitoring of individual biological traits (e.g. growth) or to repeat assays with the same individual at discrete points in time (e.g. behaviour studies). Such a method will find applications in physiology, genetics, behaviour and (eco)toxicology fields. Supplementary material available online at

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Section: Swimming Behaviourmentioning

confidence: 99%

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Cousin

Daouk

Péan

et al. 2012

Journal of Experimental Biology

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“…Zebrafish (Danio rerio) have become an important model organism for neuropharmacological and behavioural research, and they are increasingly exploited for anxiety and stress research, as their genome, brain patterning, structure and function of neurochemical and behavioural systems show large similarities to those of terrestrial vertebrates, such as humans (for a review, see Steenbergen et al (2011)). In zebrafish, the plateau of the stress response measured as full-body cortisol concentration is reached after about 15 min and recovery to baseline is accomplished after 60 min, although with a considerable amount of individual variation in the data (Ramsay et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Tudorache¹,

Schaaf²,

Slabbekoorn³

2013

Journal of Endocrinology

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All vertebrates exhibit physiological responses to a wide variety of stressors. The amplitude and profile of the response depend on the intensity, duration, controllability and predictability of the stressor, but there is also individual variation in the response, termed coping style. A better understanding of the expression of coping styles is of great value for medical applications, animal welfare issues and conservation. Here, we investigated the effect of repeated netting stress on proactive and reactive zebrafish (Danio rerio) as an upcoming model system for stress research. Fish were separated by coping styles according to the order of entering a novel environment. Subsequently, repeated netting stress was applied as stressor, over a period of 21 days. Full-body cortisol levels were determined at 0, 15, 30, 60 and 120 min after the last repeated stress event. Our results show that reactive fish display i) increased basal cortisol concentrations after being repeatedly stressed, ii) higher cortisol secretion over time and iii) slow recovery of cortisol concentration towards basal levels after the last repeated stress event. This study shows for the first time in zebrafish that different coping styles are associated with different cortisol responses during the recovery from stress over time and that coping styles can explain otherwise unaccounted variation in physiological stress responses.

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“…This is because zebrafish share extensive homologies to other vertebrate species (including rodents and humans) in terms of their genome, brain patterning, and the structure and function of several neural and physiological systems, including the stress-regulating axis (Postlethwait et al, 2000;Rodriguez et al, 2002;Tropepe and Sive, 2003;Guo, 2004;Sison et al, 2006;Lieschke and Currie, 2007;Schaaf et al, 2008;Veldman and Lin, 2008;Guo, 2009;Morris, 2009;Pogoda and Hammerschmidt, 2009;Steenbergen et al, 2010;Burne et al, 2011).…”

Section: Behavioral Readoutsmentioning

confidence: 99%

“…Recently, the zebrafish has been used as an alternative model (Steenbergen et al, 2010), and several of the traditional rodent behavioral assays have been successfully adapted and pharmacologically validated for use in zebrafish (Gerlai et al, 2000;Lockwood et al, 2004;Levin et al, 2007;Emran et al, 2008;Lopez-Patino et al, 2008;Guo, 2009;Cachat et al, 2010a,b;Champagne et al, 2010;Gerlai, 2010aGerlai, , 2010bMaximino et al, 2010aMaximino et al, ,b, 2011Steenbergen et al, 2010;Stewart et al, 2010;Stephenson et al, 2011). Recent studies have also shown the feasibility of using larval zebrafish for high throughput behavioral-based drug screening (Kokel and Peterson, 2008;Kokel et al, 2010;Rihel et al, 2010).…”

Section: Behavioral Readoutsmentioning

confidence: 99%

Zebrafish embryos and larvae: A new generation of disease models and drug screens

Ali

Champagne

Spaink

et al. 2011

Birth Defects Research Part C: Embryo Today: Reviews

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216

135

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Technological innovation has helped the zebrafish embryo gain ground as a disease model and an assay system for drug screening. Here, we review the use of zebrafish embryos and early larvae in applied biomedical research, using selected cases. We look at the use of zebrafish embryos as disease models, taking fetal alcohol syndrome and tuberculosis as examples. We discuss advances in imaging, in culture techniques (including microfluidics), and in drug delivery (including new techniques for the robotic injection of compounds into the egg). The use of zebrafish embryos in early stages of drug safety-screening is discussed. So too are the new behavioral assays that are being adapted from rodent research for use in zebrafish embryos, and which may become relevant in validating the effects of neuroactive compounds such as anxiolytics and antidepressants. Readouts, such as morphological screening and cardiac function, are examined. There are several drawbacks in the zebrafish model. One is its very rapid development, which means that screening with zebrafish is analogous to "screening on a run-away train." Therefore, we argue that zebrafish embryos need to be precisely staged when used in acute assays, so as to ensure a consistent window of developmental exposure. We believe that zebrafish embryo screens can be used in the pre-regulatory phases of drug development, although more validation studies are needed to overcome industry scepticism. Finally, the zebrafish poses no challenge to the position of rodent models: it is complementary to them, especially in early stages of drug research.

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The use of the zebrafish model in stress research

Cited by 167 publications

References 191 publications

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags

Covariation between behaviour and physiology indicators of coping style in zebrafish (Danio rerio)

Zebrafish embryos and larvae: A new generation of disease models and drug screens

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