The use of the o-nitrophenyl group as a protecting/activating group for 2-acetamido-2-deoxyglucose

Multiple-Reaction Monitoring Tandem Mass Method for Determination of Phenolics and Water-Soluble Vitamins in Eccoilopus formosanus

“…The assay of α-glucosidase inhibition was performed according to a reference method with some modifications [16]. In brief, 100 µL phosphate buffer (50 mM, pH 6.5) and a 50 µL extract were mixed with 50 µL α-glucosidase (1.5 U/mL in phosphate buffer).…”

Alfa-Glucosidase inhibitory and antioxidant activity of hexane extract of flowers, leave and stems of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech.

“…In this study, to evaluate the inhibitory effect of plant extracts on the alpha-glucosidase enzyme, Pistia-Brueggeman method was used with a few changes (Pistia-Brueggeman and Hollingsworth, 2003). 96-well microplate with 150 μl final reaction mixture volume for each well, containing 50 μl of phosphate buffer (50 mM; pH 6.8), 10 μl of alphaglucosidase (1 U/ml) and 20 μl of plant extract with various concentrations was incubated for 5 min at 37 °C, then 20 μl of 1 mM p-Nitrophenyl α-D-Glucopyranoside (pNPG) substrate was added to the mixture.…”

Influence of Rhizophora apiculata Blume extracts on α-glucosidase: Enzyme kinetics and molecular docking studies

“…α-Glucosidase inhibitory assay of R. apiculata extracts α-Glucosidase inhibitory assay was performed by the hydrolysis of chromogenic substrate PNPG (p-nitrophenyl β-D-glucoside) using 96-well microplate reader. The chromogenic substrate was hydrolyzed by the α-glucosidase enzyme resulting in the release of yellow colored p-nitrophenol, which was measured at 405 nm (Pistia-Brueggeman and Hollingsworth, 2003). Different extracts of R. apiculata each (5 mg) were dissolved in 10 ml of distilled water to obtain (500 μg/ml) of stock solution.…”