The use of the mechanical microenvironment of phospholipid polymer hydrogels to control cell behavior

Oda, Haruka; Konno, Tomohiro; Ishihara, Kazuhíko

doi:10.1016/j.biomaterials.2013.04.015

Cited by 53 publications

(52 citation statements)

References 39 publications

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“…Next, we sought to study the gels as cell encapsulants, as several studies have shown that soft hydrogels can support the retention of viable cells within polymeric matrices for cell delivery/therapy and tissue engineering applications. 60,69,72,102,[108][109][110] Optical microscopy images showed that H9c2 cells could spread evenly within the gel matrix, retained their morphology and apparently increased their density when cultured for 48 hours (Fig. 8b and c); also, the cells predominantly remained alive throughout the culture period as evidenced by the intense green fluorescent signal ( Fig.…”

Section: Self-healing Propertiesmentioning

confidence: 99%

Transiently malleable multi-healable hydrogel nanocomposites based on responsive boronic acid copolymers

2018

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The design of materials that mimic aspects of self-healing, as observed in nature, is of paramount importance as they could solve a number of problems in the biomedical field, such as the elimination of (bio-) material failure after prolonged use, improved integration at the interface with living tissues and more robust biomechanical performance. In this report, we propose the fabrication of soft hydrogels comprising a polymer matrix of poly(vinyl alcohol) mixed with a thermoresponsive boronic acid copolymer that is subsequently impregnated with poly(vinyl pyrrolidone) coated gold nanoparticles. The gels are crosslinked by the formation of reversible boronate ester bonds that can be remotely disrupted by a thermal or optical stimulus, endowing the gels with thermally-induced transient malleability that is optically trackable. It is demonstrated that the gels exhibit excellent healing properties within minutes even without the application of external stimuli. The rapid formation of the gels in concert with their fast and mild gel-sol phase transition under biologically relevant conditions is demonstrated by the encapsulation and release of cells in vitro. The proposed materials constitute a versatile cytocompatible platform for the construction of remotely healable soft gels for biomedical applications.

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Section: Self-healing Propertiesmentioning

confidence: 99%

Transiently malleable multi-healable hydrogel nanocomposites based on responsive boronic acid copolymers

2018

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“…This mild crosslinking reaction occurs in physiological environment (cell culture medium, 37°C and neutral pH) and is therefore suitable for the encapsulation of biological agents. Previous research has proved that this hydrogel enabled the safe encapsulation of bacteria [42], living cells [43][44][45], and bioactive molecules [46,47]. The PMBV/PVA hydrogel has also been used in vivo to prevent tissue adhesion surrounding tendon [48].…”

Section: Spontaneously Forming Hydrogels Designed With Phospholipid Pmentioning

confidence: 97%

“…The mechanical property of the PMBV/PVA hydrogel as a cell culture matrix was found to influence the biological functions of the encapsulated cells [45]. The storage modulus of the PMBV/PVA hydrogel could be controlled in the range of 0.30-2.5 kPa by changing the crosslinking density of the hydrogels.…”

Section: Spontaneously Forming Hydrogels Designed With Phospholipid Pmentioning

confidence: 99%

Cytocompatible and spontaneously forming phospholipid polymer hydrogels

Gao

Konno

Ishihara

2015

European Polymer Journal

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“…Figure 6 shows the distribution of these phases in the C3H10T1/2 cell cycle. [43] At the initial cell source, the cell fractions in the G1, S, and G2 phase were roughly 40%, 20%, and 40%, respectively. These fractions did not significantly change even when the cells were cultured on a usual cell culture plate.…”

Section: Cell Preservation In the Pmbv/ Pva Hydrogelmentioning

confidence: 99%

Bioinspired Phospholipid Polymer Hydrogel System for Cellular Engineering

Ishihara¹,

Oda

Aikawa

et al. 2015

Macromolecular Symposia

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Summary: The properties of the microenvironment surrounding cells are important for the control of cell functions. The polymeric cellular environment has great potential in this regard because environmental properties can be manipulated. We propose a spontaneously forming phospholipid polymer hydrogel system composed of 2 kinds of pre-polymers to control cellular functions via hydrogel physical properties. These pre-polymers are cytocompatible poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV) and water-soluble poly(vinyl alcohol) (PVA). The p-vinylphenylboronic acid units in PMBV can react with the hydroxy groups of PVA in aqueous medium and form crosslinkages. This spontaneously formed PMBV/PVA hydrogel can be dissociated again via the addition of sugar compounds. To alter the physical properties, we simply change the concentration or mixing ratio of the pre-polymers. The storage modulus of the PMBV/PVA hydrogel matrix was controlled from 0.3 kPa to 2.5 kPa, which corresponds to very soft natural tissue. Cells can be encapsulated in the hydrogel. When the storage modulus of the PMBV/PVA hydrogel was above 1.0 kPa, the proliferation of encapsulated cells was suppressed and provided uniform cells in G1 phase in cell cycle progression. High G1 phase fraction (>90%) may lead to excellent differentiation efficiency, which results in great insight for stem cell engineering. The PMBV/PVA system is expected to become a key material in cellular engineering for regulating cellular function without undesirable biological events.

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The use of the mechanical microenvironment of phospholipid polymer hydrogels to control cell behavior

Cited by 53 publications

References 39 publications

Transiently malleable multi-healable hydrogel nanocomposites based on responsive boronic acid copolymers

Transiently malleable multi-healable hydrogel nanocomposites based on responsive boronic acid copolymers

Cytocompatible and spontaneously forming phospholipid polymer hydrogels

Bioinspired Phospholipid Polymer Hydrogel System for Cellular Engineering

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