The Use of the Mass Spectrometer to Measure Deuterium in Body Fluids 1

Solomon, A. K.; Edelman, Isidore S.; Soloway, Sidney

doi:10.1172/jci102367

Cited by 43 publications

(16 citation statements)

References 14 publications

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“…Bone water was collected by vacuum distillation for 8 to 12 hours after crushing the aluminum covered samples. The details of the methods for D.0 analyses with the mass spectrometer (bone water), and with the falling drop apparatus (serum) have been published previously (31,32).…”

Section: B Resultsmentioning

confidence: 99%

Electrolyte Composition of Bone and the Penetration of Radiosodium and Deuterium Oxide Into Dog and Human Bone 12

Edelman¹,

James²,

Baden³

et al. 1954

J. Clin. Invest.

Self Cite

133

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Section: B Resultsmentioning

confidence: 99%

Electrolyte Composition of Bone and the Penetration of Radiosodium and Deuterium Oxide Into Dog and Human Bone 12

Edelman¹,

James²,

Baden³

et al. 1954

J. Clin. Invest.

Self Cite

133

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“…Method will be dealt with only briefly here. The exact laboratory details of purification, densimetric and spectrometric analysis are to be described separately (26,27). The natural abundance of deuterium is 0.0149% (28) and is approximately the same in water and naturally occurring organic substance (29,30 (31) and this amount (90 cc.)…”

Section: Introductionmentioning

confidence: 99%

The Measurement of Total Body Water in the Human Subject by Deuterium Oxide Dilution

Schloerb¹,

Friis-Hansen²,

Edelman³

et al. 1950

J. Clin. Invest.

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375

137

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“…Samples of blood for determination of the 3-hour or 24-hour equilibrium values were drawn by separate venipuncture. The concentrations of the isotopes in the blood were determined according to methods that have been described previously [deuterium with a mass spectrometer (10,11), tritium with a liquid scintillation counter (12) and radiosodium in a well-type sodium iodide (thallium) scintillation counter].…”

Section: Methodsmentioning

confidence: 99%

Absorption of Water and Sodium from the Small Intestine of Patients with Nontropical Sprue

Higgins¹,

Lee²,

Scholer³

et al. 1957

J. Clin. Invest.

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There is evidence that the absorption of water from the gastrointestinal tract of patients with nontropical sprue is delayed when they have recently eaten food (1) and also when they have fasted (2, 3). Excessive fecal loss of sodium has been demonstrated in patients with nontropical sprue (4), and recently evidence obtained by the use of isotopic sodium has indicated that the absorption of sodium chloride from the small bowel of patients with this disease is delayed (5).The present study was undertaken to confirm, if possible, the presence of a defect in the absorption of water or sodium in fasting patients with nontropical sprue and to obtain a more exact measurement of its degree. The study was made possible by the recent development of a method that allows the precise quantitative determination of the rate of absorption of isotopically labeled substances from the gastrointestinal tract of human beings under completely physiologic conditions (6). METHODSFourteen patients who presented typical clinical, laboratory and roentgenographic features of nontropical sprue were studied. Three were men and 11 were women. Their ages ranged from 23 to 63 years with the majority being in the mid-thirties. Rates of absorption were determined in most of the patients on only one occasion, the majority of these being obtained during relapses of the disease. Six tests were made during remissions.A designation of the clinical status of the disease in each patient at the time the tests were performed has been used. When loss of weight, abdominal discomfort and diarrhea or any combination of such features of the disease predominated, the patient's clinical status was termed a "relapse." When such features were absent, or nearly so, the term "remission" has been used. The tests made in the course of this study fall into two groups. In the first, the rate of absorption of water alone was measured; eight such observations were made on five patients. In the second group, the rates of absorption of both water and sodium were determined. In addition to the dual determinations, refinements in methodology developed during the progress of the second part of the study allowed more precise determination of the rates of absorption of both water and sodium.The method (6) employed for estimation of the rate of absorption of a labeled substance requires determination of its rate of appearance in the arterial blood while it is being absorbed, as well as its rate of disappearance from the arterial blood stream after its rapid intravenous injection. The precise rate of absorption of the isotope is then calculated by integration of these two rates. In the past it has been necessary to employ mean rates of arterial disappearance of the isotopes as determined in a group of healthy persons (6)(7)(8), and this was the procedure adopted in the first group of observations presented in this report. While use of a mean rate of arterial disappearance has been appropriate for the study of normal human beings, since rates of arterial disappearance vary so litt...

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The Use of the Mass Spectrometer to Measure Deuterium in Body Fluids 1

Cited by 43 publications

References 14 publications

Electrolyte Composition of Bone and the Penetration of Radiosodium and Deuterium Oxide Into Dog and Human Bone 12

Electrolyte Composition of Bone and the Penetration of Radiosodium and Deuterium Oxide Into Dog and Human Bone 12

The Measurement of Total Body Water in the Human Subject by Deuterium Oxide Dilution

Absorption of Water and Sodium from the Small Intestine of Patients with Nontropical Sprue

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