The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells

Ventura-Camargo, Bruna de Campos; Maltempi, Patrícia Pasquali Parise; Aparecida, Maria; Morales, Marin-

doi:10.4172/2161-0525.1000109

Cited by 33 publications

(35 citation statements)

References 69 publications

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“…It was suspected the workers were always exposed to azo, these substances were likely to enter and settle in the oral cavity, which could lead to cell changes, especially if the unprotected substrate was carcinogenic [16]. Studies conducted by Camargo-Ventura et al [11], the cell abnormalities caused by exposure to azo dyes showed chromosome was missing. The missing chromosome could cause inactivation effect aneugenik namely bundle mitosis.…”

Section: Discussionmentioning

confidence: 99%

“…Chemicals in the batik industry can cause irritation to skin, eyes, and cause disturbances in the respiratory system. Azo compound will be a source of disease because of its carcinogenic and mutagenic [10,11]. Previous study suggested that exposure to azo dyes significantly increasing the frequency of micronuclei [12], karyolisis [13], and pyknosis [14] in buccal mucosal epithelial cells of batik workers in Yogyakarta with the duration of exposure more than 5 years.…”

Section: Introductionmentioning

confidence: 99%

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Analyze the Expression of Cytokeratin 5 on the Epithelial Cells of the Buccal Mucosa in Batik Workers

2016

IJSR

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Abstract:The industry of batik is often use synthetic dyes as azo. Azo dyes contain naphtol and diazonium salts that are toxic to the tissue when exposed via inhalation, swallowing or direct contact. Some preliminary studies mentioned the exposure on the azo dyes batik could be expected to cause abnormalities in the buccal mucosal epithelial cell nuclei marked increase in the frequency of micronucleus, karyolisis, and pyknosis. Objective: the purpose of this study was to analyze the expression of cytokeratin 5 on the epithelial cells of the buccal mucosa in batik workers Yogyakarta. Material and Methods: the study included 30 male subjects were divided into 2 groups: 15 subjects exposed to azo dyes and 15 subjects as control. Criteria for subject were batik workers exposed azo dyes in The Unit of Coloring at batik home industry Yogyakarta, while control is not exposed to azo dyes. Buccal mucosal epithelial cells were swabbed using cytobrush. Analysis of the expression of cytokeratin 5 in the buccal mucosal epithelial cells was used monoclonal antibody cytokeratin 5 (Biocare Medical, USA) and immunohistochemical method (ABC Staining Kit, ImmunoCruz, Santa Cruz Biotechnology, USA). Data were analyzed using independent t-test. Results: the results showed the expression cytokeratin 5 of epithelial cells buccal mucosa was significantly higher in batik workers group than the control (p <0.05). Conclusion: It is concluded that exposed azo could increase the expression of cytokeratin 5 in the epithelial cells of the buccal mucosa batik workers in Yogyakarta.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analyze the Expression of Cytokeratin 5 on the Epithelial Cells of the Buccal Mucosa in Batik Workers

2016

IJSR

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“…The study of the carcinogenic and genotoxic effects of food dyes was established by some authors which gives variable results in various systems, DNA damage induced by tartrazine in rat by Himri et al (2012) and Hassan (2010), Gomes et al (2013) and Ventura-Camargo et al (2011) in Allium cepa L., AbdelMigid (2009) in Vicia faba L. and Das and Mukharjee (2004) in Salmonella. All of these studies support the data obtained in the present study and confirms the genotoxic potential of these dyes in the cells.…”

Section: Resultsmentioning

confidence: 99%

Genomic distortion induced by food dyes on meristematic cells of <i>Trachyspermum ammi</i> (L.) Sprague (Ajwain)

Dwivedi

Kumar

2017

Chromosome Botany

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Economically, food industry is one of the fastest growing industries throughout the world generating a high level of competitiveness among producers. Colour of food creates a psychological anticipation for a certain flavour To fulfill the demands of the consumers, the producers add more and more colours to the food to modify its appearance without being concerned about its adverse health effects. The objective of the present study is to evaluate the toxicity of food dyes tartrazine and sunset yellow on the mitotic cells of Trachyspermum ammi (ajwain). Both the dyes were implemented at the four doses viz. 0.5%, 1.0%, 1.5% and 2.0% in the root tip cells of T. ammi. Mitotic indices and chromosomal anomalies were calculated. Frequently observed chromosomal anomalies were stickiness, precocious movement, c-metaphase, bridges, laggards, unorientation and forward movement, etc. The result suggests the genotoxic consequences of two food dyes in the root tip cells of the test system used.

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“…Interestingly, low concentrations (5-25 µM) but longer durations of treatment cause aberrations like chromosome stickiness, breaks, and ana-/telophase bridge formation. Stickiness is reported to be an irreversible consequence of defective functioning of peripheral nuclear proteins, especially DNA topoisomerase II, interfering with chromosome segregation (Ventura-Camargo et al, 2011). Pertinently, the present Cd-treated diploid cells with high frequencies of chromosome stickiness displayed failure in free anaphase separations, resulting in chromosome bridges, laggards and breaks, or fragmentations at much higher frequency than in triploids and tetraploids.…”

Section: Effects Of CD On Antioxidant Capacitymentioning

confidence: 90%

Increasing nuclear ploidy enhances the capability of antioxidant defense and reduces chromotoxicity in Lathyrus sativus roots under cadmium stress

Talukdar¹

2014

Turk J Bot

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The modulation of antioxidant defense and cytogenetic parameters was studied in Lathyrus sativus L. roots at 3 different ploidies under a control treatment (0 µM) and 5, 10, 25, and 50 µM of CdCl 2 in nutrient media. Root growth was inhibited in diploids from 10 µM and in triploid from 25 µM. Growth was normal in tetraploid roots. Although Cd accumulated in ascending order of ploidy, perturbation of antioxidant defense was the severest in diploid, moderate in triploids, and not observed in tetraploid roots even at 50 µM. Low antioxidant enzyme activities led to overaccumulation of H 2 O 2 , consequently resulting in oxidative damage through membrane lipid peroxidation in treated diploid roots. Cd-induced chromotoxicity was exhibited by low mitotic fidelity in diploid chromosomes and to some extent in triploids. DNA base-specific fluorescent chromosome banding revealed GC to AT transition and even perturbation of the GC-rich region in the nucleolar organizing chromosome in diploid, presumably facilitating chromosome breakage at a lower ploidy level due to Cd treatment. By contrast, the normal to enhanced GC region was associated with a low percentage of chromosome breakage and other anomalies at higher ploidies exposed to Cd. Results suggest better tolerance of grass pea genotypes to Cd at elevated ploidy levels.

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The use of the Cytogenetic to Identify Mechanisms of Action of an Azo Dye in Allium Cepa Meristematic Cells

Cited by 33 publications

References 69 publications

Analyze the Expression of Cytokeratin 5 on the Epithelial Cells of the Buccal Mucosa in Batik Workers

Analyze the Expression of Cytokeratin 5 on the Epithelial Cells of the Buccal Mucosa in Batik Workers

Genomic distortion induced by food dyes on meristematic cells of <i>Trachyspermum ammi</i> (L.) Sprague (Ajwain)

Increasing nuclear ploidy enhances the capability of antioxidant defense and reduces chromotoxicity in Lathyrus sativus roots under cadmium stress

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