The Use of the Antiglobulin Test in the Diagnosis of Bovine Brucellosis

Beh, K.J.; Lascelles, A. K.

doi:10.1016/s0034-5288(18)33920-1

Cited by 25 publications

(9 citation statements)

References 6 publications

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“…peak, Immunoelectrophorctic analyses demonstrated that this activity was primarily associated with IgA. That shght enhancement of antibody activity was obtained in the antiglobnlin test is in conformity with the antibody activity being primarily IgA-associated (Beh and Lascelles, 1972). Only trace amonnts of antibody activity were associated with IgG (second O.D.…”

Section: Fractionation Of Serum and Wheysupporting

confidence: 61%

Local Antigenic Stimulation of Guinea‐pig Mammary Gland

1973

Aust J Exp Biol Med

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Summary. Local immunization of the mammary glands of pregnant guinea-pigs with killed Brucella abortus elicited a loctil production of specific antibody most of which was associated witli IgA. The local response to intra-ductal infusion of antigen was greater than that which followed injection of antigen into subcutaneous sites near the mammary gland. Local production of antibody persisted for two consecutive lactation periods following inmiunizab'on by either route.Concentrations of IgA in millc were 10-25 times greater tlian in serum, whereas the levels of IgG in milk were appro.\imately l/5th those for serum. Significantly greater amounts of IgA were present in milk from locally-infused ' glands than in milk from glands immunized by subcutaneous injection and from non-immunized glands. The levels of IgG in milk were not affected by local immunization.INTRODUCTION.

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Section: Fractionation Of Serum and Wheysupporting

confidence: 61%

Local Antigenic Stimulation of Guinea‐pig Mammary Gland

1973

Aust J Exp Biol Med

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“…Anti-Brucella antibody was measured in serum and intestinal secretion by bacterial agglutination and by the antiglobulin procedure a.s described by Beh and Lascelles (1973). Antibodies to the soluble antigens, egg albumin and hor.se spleen ferritin were detected using passive haemagglutination o£ tanned erythrocytes sensitized with antigen {Boyden, 1951).…”

Section: Antibody Assaysmentioning

confidence: 99%

The Origin of Antibody in Intestinal Secretion of Sheep

Husband

1974

Aust J Exp Biol Med

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Summary. Tbiry and double re-entrant j'ejuiia] loops were prepared in adult sheep and young lambs to study antibody responses following local and systemic administration of soluble and particulate antigens. Systemic administration of both living Brttcclta abortus and ferritin elicited an IgG response in serum and intestinal secretion. Repeated local infusions of living and killed Br. abortus into isolated intestinal loops failed to elicit a significant response. However, local infusions of egg albumin or ferritin resulted in ths appearance in intestinal secretion of substantial amounts of antibody most of which was associated with IgA. The results obtained using lambs demonstrated that local antibody production associated with IgA occurred after antigenic stimulation of intestinal loops in animals as young as 3 weeks of age.INTRODUCTION.

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“…The specificity of blocking antibodies was studied by comparing killing by normal serum and serum from infected 1 2 3 4 Reactions were determined by kinetics-based ELISA with a deoxycholate extract of B. abortus as the antigen. Data points represent percent increase in antibody concentration over the mean of four preinfection samples.…”

Section: Discussionmentioning

confidence: 99%

Killing of Brucella abortus by bovine serum

et al. 1988

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Studies of the serum bactericidal system in bovine brucellosis were undertaken to investigate the role of the humoral immune response in protection of cattle against the facultative intracellular parasite Brucella abortus. Fresh sera from normal control cattle, infected cattle, and cattle immunized with B. abortus cell envelopes were collected before treatment and during the course of immunization or infection. Normal fresh bovine serum or fresh agammaglobulinemic serum from colostrum-deprived calves was effective in killing smooth virulent B. abortus 2308, but rough strains RB51 (a rough mutant of strain 2308) and 45/20 were much more senitive to serum. The difference in susceptibility to serum was shown to be correlated with differences in lipopolysaccharide chemotype, with the more resistant strain 2308 having 0 polysaccharide and the more susceptible strains 45/20 and RB51 lacking 0 side chains. By treatment of fresh serum with MgCl2 and EGTA [ethylene glycol-bis(IO-aminoethyl ether)-N,N,N',N'-tetraacetic acid] killing was shown to occur via the classical pathway of complement activation. When antibody to B. abortus was present, killing of strain RB51 increased but killing of smooth strain 2308 decreased. The earliest antibody response in serum from infected animals did not interfere with killing. When affinity-purified bovine immunoglobulins specific for B. abortus smooth lipopolysaccharide were added to fresh normal bovine serum, immunoglobulin Gl (IgGl) and IgG2 isotypes blocked killing but IgM and IgA isotypes did not. Thus, it appears that serum from previously unexposed animals or animals early during infection can kill smooth B. abortus, an appropriate defense mechanism before the organism becomes intracellular. At later stages of infection, blocking antibodies predominate.

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The Use of the Antiglobulin Test in the Diagnosis of Bovine Brucellosis

Cited by 25 publications

References 6 publications

Local Antigenic Stimulation of Guinea‐pig Mammary Gland

Local Antigenic Stimulation of Guinea‐pig Mammary Gland

The Origin of Antibody in Intestinal Secretion of Sheep

Killing of Brucella abortus by bovine serum

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