The Use of Telomerase Activity for the Detection of Prostatic Cancer Cells After Prostatic Massage

Meid, Florian; Gygi, Christian M.; Leisinger, H.-J.; Bosman, Fred T.; Benhattar, Jean

doi:10.1016/s0022-5347(05)66417-8

Cited by 54 publications

(19 citation statements)

References 17 publications

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“…However, these cell populations are only weakly positive for telomerase activity. This observation is consistent with the results of a previous report, in which it was demonstrated that specimens associated with the foci of chronic and subacute inflammation were negative for TRAP assay (Meid et al 2001). In addition, although it is well known that many prostate glands of BPH patients are often the site of chronic inflammatory responses, several studies have failed to show any telomerase activity in histologically confirmed BPH samples obtained from patients with BPH.…”

Section: Discussionsupporting

confidence: 93%

“…Our aim was to verify the applicability of this method to the detection of telomerase activity in patients with prostatic proliferative diseases. Similar studies have been performed by others (Wang et al 2000;Meid et al 2001); however, some specific differences exist in the experimental design of our study. We admitted to the study a selected series of 30 patients with histological proven Pca, and 30 patients with BPH who were at least 50 years old, had a biopsy and clinical diagnosis of either BPH or Pca, had clinical evidence of LUTS, had no suspicious DRE for cancer, had no specific TRUS appearance for Pca, had moderately elevated total PSA values, and had no other urological cancers.…”

Section: Discussionsupporting

confidence: 50%

“…In recent years, in order to avoid serially repeated invasive analyses, many investigators have begun to assay telomerase activity in body fluids that may contain tumour cells. These fluid samples, recovered from breast cancer (Poremba et al 1999), pleural effusion (Yang et al 1998), pancreatic cancer (Hiyama et al 1997), and prostatic cancer (Meid et al 2001) can be obtained through non-invasive methods, and have demonstrated that the combination of telomerase analysis in cytological specimens may result in more accurate diagnoses.…”

Section: Introductionmentioning

confidence: 99%

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Detection of telomerase activity in prostate massage samples improves differentiating prostate cancer from benign prostatic hyperplasia

Vicentini

Gravina

Angelucci

et al. 2004

J Cancer Res Clin Oncol

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Our data indicate that telomerase activity detected by TRAP assay on prostate epithelial cells collected by prostate massage can substantially improve the distinction between Pca and BPH conditions. One of the clinical benefits resulting from the use of this new assay would be to refine the biopsy indication and to avoid for several patients without Pca the unnecessary cost and the complications of prostate biopsy.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of telomerase activity in prostate massage samples improves differentiating prostate cancer from benign prostatic hyperplasia

Vicentini

Gravina

Angelucci

et al. 2004

J Cancer Res Clin Oncol

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“…High telomerase activity has been found in 90% of prostate cancers and was shown to be absent in normal prostate tissue. In a small study [36] of 36 specimens, telomerase activity was used to detect prostate cancer cells in voided urine or urethral washing after prostate massage. This test had a sensitivity of 58% and a specificity of 100%.…”

Section: Telomerasementioning

confidence: 99%

Molecular prostate cancer pathology: Current issues and achievements

Schalken¹,

Bergh

Bono

et al. 2005

Scandinavian Journal of Urology and Nephrology

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Recent developments in the field of molecular techniques have provided new tools that have led to the discovery of many new promising biomarkers for prostate cancer. These biomarkers may be instrumental in the development of new tests that will have a high specificity for the diagnosis and prognosis of prostate cancer. A biomarker is defined as a molecular test that provides additional information to currently available clinical and pathological tests. Biomarkers should be reproducible (both within and between institutes) and have an impact on clinical management. For diagnostic purposes it is important that potential biomarkers are tested in terms of tissue specificity and their discrimination potential between prostate cancer, normal prostate and benign prostatic hyperplasia. The results of (multiple) biomarker-based assays may enhance the specificity of cancer detection. There is an urgent need for molecular prognostic biomarkers for predicting the biological behavior and outcome of cancer.

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“…Eine mögliche Alternative zum Nachweis von Prostatakarzinomen stellt der Nachweis der Telomeraseaktivität in Exprimaturinproben dar.Mittels des TRAP-Assays wurde eine Sensitivität von 58% bei einer Spezifität von 100% erreicht [26].Die oben erwähnten Probleme des TRAP-Assays bei Urinproben sprechen eher für den Nachweis der GSTP1-Promotorhypermethylierung.…”

Section: Telomeraseaktivität In Exprimaturinunclassified

Molekulare Diagnostik in der urologischen Onkologie

et al. 2003

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The goal of molecular diagnostics in oncology is the early diagnosis of malignant disease processes during initial work-up or as part of follow-up. Body fluids serve as the primary material for non-invasive diagnostic methods. Besides actual tumor cells, the examination of urine can yield evidence of secreted proteins or even free nucleic acids. In principle, all of the methods available for the detection of tumor markers in tissue or blood samples can be successfully applied to the examination of urine samples. However, molecular biological examination of urine samples is associated with important problems because the cells in such samples are exposed to significant degradation and regression effects and because certain components of the urine act to inhibit the polymerase chain reaction. The present overview discusses the respective strengths and weakness of the available technology as applied to the diagnosis of urologic malignancies. Experimental studies conducted to date have reported high sensitivities and specificities for molecular diagnostics using urine samples. It is important to note that not only carcinomas of the urinary bladder can be diagnosed from material obtained in urine samples: in fact, the method can be used to diagnose entities such as renal cell and prostate carcinomas and, due to renal filtration of DNA, even non-urologic malignancies. The diagnostic application of these methods, however, remains in an experimental stage and must still clear several hurdles before becoming available for routine clinical use.

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The Use of Telomerase Activity for the Detection of Prostatic Cancer Cells After Prostatic Massage

Cited by 54 publications

References 17 publications

Detection of telomerase activity in prostate massage samples improves differentiating prostate cancer from benign prostatic hyperplasia

Detection of telomerase activity in prostate massage samples improves differentiating prostate cancer from benign prostatic hyperplasia

Molecular prostate cancer pathology: Current issues and achievements

Molekulare Diagnostik in der urologischen Onkologie

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