The Use of Tannic Acid-Glutaraldehyde in the Study of Elastic and Elastic-Related Fibers

Cotta-Pereira, Gerson; Fg, Rodrigo; Jf, David-Ferreira

doi:10.3109/10520297609116662

Cited by 134 publications

(78 citation statements)

References 8 publications

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“…The ventral prostates of control and treated gerbils were processed for transmission electron microscopy as described previously (De Carvalho et al, 1994), employing the fixation procedure of Cotta-Pereira et al (1976). Briefly, tissue fragments were fixed in 0.25% tannic acid plus 3% glutaraldehyde in Millonig's buffer, dehydrated in acetone, and embedded in Araldite resin.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Tissue evidence of the testosterone role on the abnormal growth and aging effects reversion in the gerbil (Meriones unguiculatus) prostate

2006

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Prostate differentiation during embryogenesis and its further homeostatic state maintenance during adult life depend on androgens. Abundant biological data suggest that androgens play an important role in the development of the prostate cancer and other prostatic diseases. The objective of this work was to evaluate the effects of the testosterone supplementation in gerbil (a new experimental model) at different ages. Tissues from experimental animals were studied by histological and histochemistry procedures, androgen receptor immunohistochemistry assay, morphometricstereological analysis, and transmission electron microscopy (TEM). After the treatment were observed increase of prostate weight and epithelium height in all ages studied. In some adult and aged treated animals, hyperplasic and displasic process were observed, including prostatic intraepithelial neoplasias and adenocarcinomas. Increase of the thickness of the smooth muscle cell (SMC) layer was observed in pubescent and adult animals and TEM revealed apparent SMC hypertrophy. An apparent increase in the frequency of blood vessels distributed by the subepithelial stroma in the treated animals was noticed. Reversion of the natural effects of aging on the prostate was observed in the aged treated animals in some acini of the gland. These data demonstrate that the gerbil prostate is susceptible to androgenic action at the studied ages and it can serve, for example, as experimental model to studies of prostate neoplasic process induction and hormonal therapy in aged animals. Anat Rec Part A, 288A:1190-1200, 2006.2006 Wiley-Liss, Inc.Key words: testosterone; prostate; stroma; epithelium; gerbil Androgens are steroid hormones that induce the differentiation and maturation of the male reproductive organs and the development of the male secondary sex characteristics. Prostate differentiation during embryogenesis and its further homeostatic state maintenance during adult life depend on androgens. The normal prostatic epithelium is composed of different cells types that have varying androgen sensitivities, including androgen-independent basal stem cells, androgen-dependent luminal secretory cells, and androgen-independent but androgen-sensitive

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Section: Transmission Electron Microscopymentioning

confidence: 99%

Tissue evidence of the testosterone role on the abnormal growth and aging effects reversion in the gerbil (Meriones unguiculatus) prostate

2006

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“…Strains were cultured and maintained under 20 C ± 1.50 µmol photons m À2 s À1 irradiance and 14 : 10 h light: dark cycle in the Culture Collection of IBILCE/UNESP. The samples were fixed by immersion in 3 % glutaraldehyde plus 0.25 % tannic acid solution in Millonig's buffer, pH 7.3, containing 0.54 % glucose for 24 h. After washing with the same buffer, they were post-fixed with 1 % osmium tetroxide for 2 h, washed again, dehydrated in a graded acetone series and embedded in Araldite resin (Cotta-Pereira et al, 1976). Ultrathin sections (50-75 nm) were cut using a diamond knife and contrasted with 2 % uranyl acetate for 30 min (Watson, 1958), followed by 2 % lead citrate in sodium hydroxide solution for 10 min (Venable & Coggeshall, 1965).…”

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confidence: 99%

Proposal of Ancylothrix gen. nov., a new genus of Phormidiaceae (Cyanobacteria, Oscillatoriales) based on a polyphasic approach

Martins

Rigonato

Taboga

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…After washing with the same buffer, they were postfixed with 1% osmium tetroxide for 2 hr, washed again, dehydrated in graded acetone series, and embedded in Araldite resin (Cotta-Pereira et al, 1976). Ultrathin sections (range, 50-75 nm) were cut using a diamond knife and contrasted with 2% uranyl acetate for 30 min (Watson, 1958), followed by 2% lead citrate in sodium hydroxide solution for 10 min (Venable and Coggeshall, 1965).…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Testosterone Promotes an Anabolic Increase in the Rat Female Prostate (Skene's Paraurethral Gland) Which Acquires a Male Ventral Prostate Phenotype

Biancardi

Santos

Madi-Ravazzi

et al. 2010

The Anatomical Record

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The female prostate (Skene's paraurethral gland) in the rat is morphologically similar to the ventral lobe of male adults and has been described in other rodent species and humans. Previous studies on prostate morphogenesis suggest that female Wistar rats (Rattus norvegicus) do not develop this gland due to the absence of testosterone during the embryonic and neonatal periods. On the other hand, studies conducted in our laboratory have shown that some females of this species can present an undeveloped but functional prostate. Recent studies on this gland have caused scientific interest because, besides being active in the processes of synthesis and secretion of prostatic material, it is also targeted by both malignant and benign lesions, mainly during senescence. Thus, this work aims to evaluate the structure of female prostate of adult rats (Rattus norvegicus) under normal conditions and under the effect of testosterone treatment and carry out comparative studies on the ventral prostate of young and adult male rats. Morphological and morphometric stereological analyses and immunocytochemical and ultrastructural studies were conducted. The results have shown that the prostate gland of rats exposed to androgen therapy have experienced intense growth, becoming more active in relation to synthesis and secretion. It may be concluded that the prostate in control adult female rats is morphologically very similar to the prostatic ventral lobe of young male rats. Besides, under androgenic action, the female prostate grows considerably and becomes similar to the prostatic ventral lobe in male adults.

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The Use of Tannic Acid-Glutaraldehyde in the Study of Elastic and Elastic-Related Fibers

Cited by 134 publications

References 8 publications

Tissue evidence of the testosterone role on the abnormal growth and aging effects reversion in the gerbil (Meriones unguiculatus) prostate

Tissue evidence of the testosterone role on the abnormal growth and aging effects reversion in the gerbil (Meriones unguiculatus) prostate

Proposal of Ancylothrix gen. nov., a new genus of Phormidiaceae (Cyanobacteria, Oscillatoriales) based on a polyphasic approach

Testosterone Promotes an Anabolic Increase in the Rat Female Prostate (Skene's Paraurethral Gland) Which Acquires a Male Ventral Prostate Phenotype

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