The use of synchrotron infrared microspectroscopy to demonstrate the effect of intense pulsed light on dermal fibroblasts

Abstract

Intense pulsed light (IPL) has been used extensively to treat striae distensae (SD). Twenty-four patients (21 females and 3 males) with age ranging between 15-42 years with a mean of 24.2 ± 5.89 years were included in this study. All patients were treated with IPL for five sessions and were given 4-week interval between the sessions. Formalin-fixed, paraffin- embedded skin biopsies were cut into 5-μm-thick sections from patients before and after treatments. They were stained with hematoxylin and eosin (H&E) and collagen with Masson trichrome stain. Histological examination of SD revealed epidermal thinning, flattened rete ridges, and fragmentation with degeneration of the collagen and elastic fibers. After IPL treatments, collagen expression was found also to be increased in a highly significant manner and P values were < 0.001 after IPL treatments. The use of synchrotron infrared (IR) microspectroscopy also revealed that amide 1 and beta sheets were expressed in a significant manner after IPL treatment. In conclusion, it was demonstrated that IPL can treat striae through fibroblast stimulation which led to protein expression especially collagen stimulation, and it seems that it is a promising therapeutic modality for striae with minimal side effects.

Evaluation of a non‐ablative, fractional 1565 nm laser for the improvement of striae distensae albae

“…S v , indicating depth of atrophic lesions, decreased from baseline mean score of À285.0 AE 63.66 lm to À194.6 AE 37.32 lm at 1-month FU, with À203.8 AE 33.09 lm at 6-month FU (Relative change at 1month FU compared to baseline of 31.7%; relative change at 6month FU of 28.5%; P = <0.001, respectively). We observed a decrease in maximum expansion of skin roughness, with S max at 18.1% at 1-month FU compared to baseline; relative change at 6-month FU of 33.7% to baseline; P = <0.01, respectively). Furthermore, an overall more plane surface was calculated, with S dr : baseline score of 9.64 AE 4.06%, 4.44 AE 1.10% at 1-month FU and 4.13 AE 1.23% at 6-month FU (Figs 5 and 6).…”

“…[15][16][17] However, various studies using light and laser techniques showed promising outcome in the improvement of SD. 2,6,18 Intense pulsed light (IPL), with visible beam of highintensity light, increased dermal collagen level and led to skin tightening. [18][19][20] A broad range of laser devices has been utilized for the treatment of SD with variable outcome.…”

“…2,6,18 Intense pulsed light (IPL), with visible beam of highintensity light, increased dermal collagen level and led to skin tightening. [18][19][20] A broad range of laser devices has been utilized for the treatment of SD with variable outcome. 585 nm pulsed dye laser (PDL) with its high affinity for haemoglobin is most commonly used in SD rubrae to reduce vascularity, with minimum benefit in the treatment of SD albae.…”

Fractional CO2laser versus intense pulsed light in treating striae distensae

“…Intense pulsed light (IPL) was used to treat striae distensae with good results. [ 16 ] A previous study has demonstrated that IPL replaces dermal elastosis with neo-collagen. [ 17 ]…”

The Efficacy of IPL in Periorbital Skin Rejuvenation: An Open-Label Study

“…The effectiveness of IPL in treating skin wrinkles is attributed to the creation of microthermal damage in the dermis resulting in increased collagen production in a process similar to that seen in wound healing. 13,14 Pathology specimens obtained after IPL treatment show enhanced collagen and elastic fibre formation in the papillary and sub-papillary dermis, resulting in an increase in volume which in turn leads to smoothing the upper dermal layers and the increased epidermal turnover. 15,16 In an in vitro study, Wong et al 17 showed that after IPL irradiation, the number of live fibroblasts increases more than 100%.…”