The use of sorghum for thermostable amylase production from
            <b>
              <i>Thermoactinomyces thalpophilus</i>
            </b>

Uguru, Gabriel C.; Akinyanju, J. A.; Sani, A.

doi:10.1046/j.1472-765x.1997.00158.x

Cited by 9 publications

(8 citation statements)

References 3 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This disparity could be due to the ability of the fungal species to adapt to and survive in harsh conditions which are characteristics of the lignocellulosic waste dumping sites. This agrees with findings by Ajijolakewu and Sani (2012); Ray (2004) and Uguru et al (1997).…”

Section: Microbial Profile Of Soil Samples Collected Fromsupporting

confidence: 93%

Cellulase Production Potentials of the Microbial Profile of Some Sugarcane Bagasse Dumping Sites in Ilorin, Nigeria

et al. 2013

View full text Add to dashboard Cite

This research work investigated cellulase production potentials of the microbial profile of three sugarcane bagasse dumping sites at Zango area, Ilorin, Nigeria. The microbial isolates were screened for cellulase production with a view to select the best organism for eventual cellulase production. Pour Plate method was used for the isolation and a total of thirteen (13) different organisms including both fungal and bacterial species were isolated and screened. Six (6) fungal isolates identified as Mucor racemosus Aspergillus niger, Aspergillus flavus, Neurospora sitophilus, Penicillium oxalicum and Penicillium citrinum were isolated, while seven (7) different bacterial species isolated include Clostridium cellobioparum, Clostridium thermocellum, Bacillus subtilis, Bacillus pumillus, Lactobacillus spp, Pseudomonas flavescens and Serratia spp. Generally, bacterial isolates were more in abundance than fungal species. However; fungal isolates were constant and were isolated through the experimental period of three weeks. All the isolates showed cellulase production potential in varying degrees as reflected in the clearance zone around their colonies. Fungal isolates produced more cellulase than the bacterial isolates. Mucor racemosus had the highest clearance zone (75.0 mm) among the fungal isolates while Clostridium cellobioparum (35.0 mm) were the best producer among bacterial isolates. The least producer among fungal isolates, Penicillium citrinum (40.0 mm), is a little more than the bacterial cellulase producer (35.0 mm) and is far greater than the least bacterium Serratia spp (14.0 mm).

show abstract

Section: Microbial Profile Of Soil Samples Collected Fromsupporting

confidence: 93%

Cellulase Production Potentials of the Microbial Profile of Some Sugarcane Bagasse Dumping Sites in Ilorin, Nigeria

et al. 2013

View full text Add to dashboard Cite

show abstract

“…A similar result was obtained by Antrim et al [ 36 ] who reported a thermostable α-amylase with activity at pH 5.5. Uguru et al [ 37 ] isolated a strain of Thermoactinomyces thalpophilus which produced an extracellular amylase with optimum pH of 5.0. The thermostable amylases have extensive applications in a number of industrial processes [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Statistical factorial designs for optimum production of thermostable α-amylase by the degradative bacterium Parageobacillus thermoglucosidasius Pharon1 isolated from Sinai, Egypt

Saeed

El-Shatoury

Sayed

2021

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background This study aimed to isolate potent thermophilic and amylolytic bacteria from a hot spring of Pharaoh’s bath, Sinai, Egypt, and screen its degradative activity. The amylolytic activity was further optimized using a statistical full factorial design followed by response surface methodology. Results A thermophilic bacterium was isolated from the hot spring of Pharaoh’s Bath, Sinai, Egypt. The isolate produced amylase, cellulase, and caseinase and was identified by 16S rRNA gene sequencing as Parageobacillus thermoglucosidasius Pharon1 (MG965879). A growth medium containing 1% soluble starch was found to optimize the amylase production. Dinitrosalycalic acid method (DNS) was used to estimate the amount of reducing sugar produced. Statistical full factorial and response surface designs were employed to optimize physical variables affecting the α-amylase production and determine the significant interactions of the studied variables during the fermentation process. According to the results obtained by the response optimizer, the maximum amylase activity reached 76.07 U/mL/ min at 54.1°C, pH 5.6 after 98.5 h incubation under aerobic conditions. Moreover, the produced enzyme was thermostable and retained most of its activity when exposed to a high temperature of 100°C for 120 min. Maximum enzyme activity was attained when the enzyme was incubated at 70°C for 30 min. Conclusions This is the first report of the production of thermostable α-amylase by the potent thermophilic Parageobacillus thermoglucosidasius. The enzyme endured extreme conditions of temperature and pH which are important criteria for commercial and industrial applications.

show abstract

“…In addition, -amylase activity of Thermoactinomyces species was first reported by Kuo & Hartman (1966). After that, severalamylases with different characters were found in other studies (Obi & Odibo, 1984;Omar et al, 2011;Shimizu et al, 1978;Uguru et al, 1997). Within actinomycetes, available reports on -amylase production are scanty and refer mainly to nonthermostable enzyme (Shinke et al, 1974).…”

Section: Genes Encoding Intracellular -Amylases Have Been Reported Fomentioning

confidence: 96%

Starch and Microbial α-Amylases: From Concepts to Biotechnological Applications

El‐Fallal¹,

Abou‐Dobara²,

El-Sayed³

et al. 2012

Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology

View full text Add to dashboard Cite

The use of sorghum for thermostable amylase production from Thermoactinomyces thalpophilus

Cited by 9 publications

References 3 publications

Cellulase Production Potentials of the Microbial Profile of Some Sugarcane Bagasse Dumping Sites in Ilorin, Nigeria

Cellulase Production Potentials of the Microbial Profile of Some Sugarcane Bagasse Dumping Sites in Ilorin, Nigeria

Statistical factorial designs for optimum production of thermostable α-amylase by the degradative bacterium Parageobacillus thermoglucosidasius Pharon1 isolated from Sinai, Egypt

Starch and Microbial α-Amylases: From Concepts to Biotechnological Applications

Contact Info

Product

Resources

About