The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression

Melo, Luciana Magalhães; Silva, Sâmara B.; Magalhães, L. C.; Cortez, Jenin V.; Kumar, Satish; Duarte, José Maurício Barbanti; Rola, Luciana Diniz; Chaves, Maiana Silva; Freitas, Vicente José de Figueirêdo

doi:10.1016/j.therwi.2022.100001

Cited by 3 publications

(2 citation statements)

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“…This showed that the cow and Oryx have a healthy nucleus–cytoplasm connection. The results of our study showed superior results to those obtained in other studies of interspecific somatic cell nuclear transfer in different animals, such as [ 25 , 28 , 52 , 53 , 54 , 55 , 56 , 57 ]. Producing animals by interspecific somatic cell nuclear transfer presents a variety of challenges, including incompatibility of mitochondrial/genomic DNA, activation of the nucleus’ embryonic genome by the donor oocyte, the lack of fosters who are appropriate for interspecific somatic cell nuclear transfer embryos, and epigenetic disorders and unstable gene expression formations [ 58 , 59 ].…”

Section: Discussionsupporting

confidence: 39%

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Ammari

Al-Ghadi

Amran

et al. 2022

Veterinary Sciences

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Cloning, commonly referred to as somatic cell nuclear transfer (SCNT), is the technique of enucleating an oocyte and injecting a somatic cell into it. This study was carried out with interspecific SCNT technology to clone the Arabian Oryx utilizing the oryx’s fibroblast cells and transfer it to the enucleated oocytes of a domestic cow. The recipient oocytes were extracted from the cows that had been butchered. Oryx somatic nuclei were introduced into cow oocytes to produce embryonic cells. The study was conducted on three groups, Oryx interspecific somatic cell nuclear transfer into enucleated oocytes of domestic cows, cow SCNT “the same bovine family species”, used as a control group, and in vitro fertilized (IVF) cows to verify all media used in this work. The rates of different embryo developmental stages varied slightly (from 1- cell to morula stage). Additionally, the oryx interspecies Somatic cell nuclear transfer blastocyst developmental rate (9.23%) was comparable to that of cow SCNT (8.33%). While the blastula stage rate of the (IVF) cow embryos exhibited a higher cleavage rate (42%) in the embryo development stage. The results of this study enhanced domestic cow oocytes’ ability to support interspecific SCNT cloned oryx, and generate a viable embryo that can advance to the blastula stage.

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Section: Discussionsupporting

confidence: 39%

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Ammari

Al-Ghadi

Amran

et al. 2022

Veterinary Sciences

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“…Recent potential interest has arisen in the use of enucleated GV, MI and MII oocytes as recipientscytoplasts for GV nucleus, embryonic and somatic nuclei in addition to germ cells in the last couple of decades for different purposes (Mohammed et al, 2019;Melo et al, 2022). The stages of enucleated oocyte include germinal vesicle, metaphase I and metaphase II stage oocytes.…”

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confidence: 99%

Do the Mammalian Artificial Oocytes Repair Reproductive Dysfunctions in Mammalian Species?: A Review

Mohammed,

AlGherair,

Al-Suwaiegh

et al. 2024

IJAR

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Artificial oocytes were used of different mammalian species for repairing reproductive dysfunctions. Artificial oocytes are those created in vitro using enucleated germinal vesicle, metaphase I and metaphase II oocytes followed by embryonic cells, somatic cells, pluripotent stem cells and germ cells nuclear transfer. Artificial oocytes are under development, they have the potential to revolutionize reproduction in mammalian species. The potential applications of artificial oocytes include 1) treatment of infertility as premature ovarian failure, ovarian cancer, or other conditions that damage the ovaries, 2) preservation of fertility, 3) creating cloned and transgenic animals. While artificial oocytes have the potential to revolutionize reproductive medicine, there are still a number of challenges that need to be addressed before they can be effectively and safely used in humans including the adapting efficient and safe enucleation techniques. Furthermore, cellular reprogramming is the biggest obstacle for creating artificial oocytes in addition to activation procedures to ensure that artificial oocytes are genetically and epigenetically normal and producing healthy offspring. Several studies are designed to overcome these challenges and there is significant progresses being made. Offspring are obtained in several animals species whereas developed blastocysts were obtained in humans. This review is developed and implemented to discuss the progress in creation of “artificial oocytes” in addition to the factors affecting the developmental competence of reconstructed oocytes.

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Strategies for the Establishment of Fibroblastic Lines for the Conservation of Wild Mammals

Fernandes Pereira,

Ricarliany Medeiros de Oliveira,

Vitorino Costa de Aquino

et al. 2023

Veterinary Medicine and Science

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The loss of wild biodiversity has encouraged the development of fibroblastic lines, mainly fibroblasts derived from skin, which can be interesting tools for the conservation of wild mammals. These biological samples, when properly well-established, are essential elements for the reproduction of species through their use in cloning by somatic cell nuclear transfer and induction of cells to pluripotency. In general, the establishment of fibroblastic lines involves the following strategies: (i) cell isolation techniques and identification of fibroblasts; (ii) conditions for in vitro culture of fibroblasts; (iii) conditions for cryopreservation of fibroblasts; and (iv) nuclear reprogramming studies. At each stage, species-specific factors are involved, and determining these lines in the species of interest represents the first step toward its successful use for animal conservation. Therefore, this chapter discusses the stages and parameters involved in the strategies for establishing fibroblastic lines, delving into the main technical aspects and results obtained from the use of these cells in recent years in wild mammals.

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The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression

Cited by 3 publications

References 53 publications

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Do the Mammalian Artificial Oocytes Repair Reproductive Dysfunctions in Mammalian Species?: A Review

Strategies for the Establishment of Fibroblastic Lines for the Conservation of Wild Mammals

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