The Use of Solid Media for Detection of Enzyme Production by Fungi

Hankin, Lester; Anagnostakis, Sandra L.

doi:10.2307/3758395

Cited by 373 publications

(286 citation statements)

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“…Five days after incubation at ±25°C and 12 h photoperiod, plates were flooded with 1 mL of iodine solution and a white zone formed in the colony indicated the fungus ability to degrade starch (Hankin and Anagnostakis 1975). Fungal growth and starch degradation area were calculated using the formula:.…”

Section: Methodsmentioning

confidence: 99%

“…For lipolytic activity, fungus was grown in culture medium supplemented with 10 g L −1 peptone, 5 g L −1 NaCl, 0.1 g L −1 CaCl 2 .2H 2 O and 1% Tween® 20 (pH 7.4) (Hankin and Anagnostakis 1975). For proteolytic activity, cultures were grown on agar medium supplemented with 0.4% of soluble skim milk (Liao et al 2012).…”

Section: Methodsmentioning

confidence: 99%

“…In order to detect pectate lyase (PL EC 4.2.2.2) pH medium was adjusted to 7.0 and, for polygalacturonase (PG EC 3.2.1.15) it was adjusted to 5.0 (Hankin and Anagnostakis 1975). Five days after incubation, pectolytic enzymes were determined by measuring the size of halos (mm) around the colonies after flooding them with 10 g L −1 of Hexadecyltrimethylammonium bromide (CTAB) solution for 10 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Extracellular enzymes ofColletotrichum fructicola isolates associated to Apple bitter rot and Glomerella leaf spot

2018

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Colletotrichum fructicola causes two important diseases on apple in Southern Brazil, bitter rot (ABR) and Glomerella leaf spot (GLS). In this pathosystem, the Colletotrichum ability to cause different symptoms could be related to differences of extracellular enzymes produced by the fungi. Thus, the objectives of this study were to compare the production of these enzymes between ABR- and GLS-isolate in vitro and to evaluate their involvement on infected apple leaves with C. fructicola. In agar plate enzymatic assay, ABR- showed significantly higher amylolytic and pectolytic activity than GLS-isolate. In contrast, for lipolytic and proteolytic no significant differences were observed between isolates. In culture broth, ABR-isolate also had higher activity of pectin lyase (PNL), polygalacturonase (PG) and laccase (LAC). Notably, LAC was significantly five-fold higher in ABR- than GLS-isolate. On the other hand, in infected apple leaves no significant difference was observed between isolates for PNL, PG and LAC. Although differences in extracellular enzymes of ABR- and GLS-isolate have not been observed in vivo, these results contributed to highlight the importance to investigate such enzymes in depth.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Extracellular enzymes ofColletotrichum fructicola isolates associated to Apple bitter rot and Glomerella leaf spot

2018

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“…Screening for protease activity was performed on prepoured Czapek Dox agar plates supplemented with 1% casein (w/v) by point inoculation with mycelium/spores of fungal isolates [15]. Petri plates were stained with Nessler's reagent and the hydrolyzing zones were measured and photographed.…”

Section: Screening For Proteolytic Activitymentioning

confidence: 99%

Effect of cypermethrin, a pyrethroid insecticide on dynamics of soil microflora

Sethi¹,

Pradhan²,

Behera³

et al. 2015

J App Biol Biotech

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“…The isolates of endophytic fungi were tested for their ability to produce the hydrolytic enzymes i.e., cellulase, amylase, protease, lipase and pectinase [23,24]. Quantitative estimation of proteolytic activity [25] and chitinase [26] was carried out.…”

Section: Qualitative and Quantitative Extracellular Enzyme Assaymentioning

confidence: 99%

In Vitro Evaluation of Antagonism of Endophytic Colletotrichum gloeosporioides Against Potent Fungal Pathogens of Camellia sinensis

et al. 2014

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An endophytic fungus isolated from Camellia sinensis, Assam, Northeastern India was identified as Colletotrichum gloeosporioides on the basis of morphological characteristics and rDNA ITS analysis. This endophytic fungus was evaluated for growth inhibition against tea pathogens Pestalotiopsis theae and Colletotrichum camelliae. One isolate of C. gloeosporioides showed strong antagonistic activity against Pestalotiopsis theae (64 %) and moderate activity against C. camelliae (37 %). Fifty percent cell-free culture filtrate from 5-day-old cultures showed highest antagonistic activity against both the pathogens although the inhibition percent was less as compared to dual culture. In the experiment of volatile compounds none of the isolates of C. gloeosporioides strains showed visible inhibition against P. theae and C. camelliae. The activity of extracellular hydrolytic enzymes chitinase and protease was also high in this culture fluid and measured 10 and 4.3 IU/ll, respectively.

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The Use of Solid Media for Detection of Enzyme Production by Fungi

Cited by 373 publications

References 0 publications

Extracellular enzymes ofColletotrichum fructicola isolates associated to Apple bitter rot and Glomerella leaf spot

Extracellular enzymes ofColletotrichum fructicola isolates associated to Apple bitter rot and Glomerella leaf spot

Effect of cypermethrin, a pyrethroid insecticide on dynamics of soil microflora

In Vitro Evaluation of Antagonism of Endophytic Colletotrichum gloeosporioides Against Potent Fungal Pathogens of Camellia sinensis

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