The use of single-donor fibrin glue prepared by recycled cryoprecipitation in experimental liver surgery

Abstract

The purpose of the study was to evaluate the hemostatic effectiveness of fibrin glue (FG) prepared by a modification of cryoprecipitation technique in experimental rat liver surgery. FG component 1 was prepared by triple or 'recycled' cryoprecipitation method from single-donor plasma. Rats subjected to liver incision, partial and total lobectomy were treated with FG on the surgical cut surface or underwent standard surgical technique. The efficacy of FG-treatment was evaluated on the basis of the 24-hour survival ratio and peripheral blood hematological parameters. The mean values of fibrinogen, FXIII, fibronectin and horizontal tensile strength of FG were 54.2 +/- 19.9 g/l, 13.5 +/- 3.6 IU/ml, 3103.1 +/- 148.9 mg/l, and 1.076 +/- 0.18 N/cm2, respectively. The survival of FG-treated rats subjected to partial and total lobectomy was significantly higher in comparison to the FG-nontreated animals, accompanied with higher values of red blood cell counts, hemoglobin concentration and hematocrit. When liver incision was performed, although there were no differences in survival rate, FG-treated animals had significantly higher values of the tested hematological parameters. The presented results demonstrated that by using 'recycled' cryoprecipitation it is possible to obtain high quality single-donor FG with successful hemostatic therapeutical effects, as confirmed in the experimental rat model of liver surgery.

Reconstruction of the mandible with an autogenous corticocancellous bone graft and fibrin glue: An animal experiment

“…Many authors indicate the efficacy of FL usage in cardio surgery, abdominal surgery, neurosurgery, oral surgery etc. (Balint, 1996, Balint, 2002. Tayapongsak et al (1994) described the use of fibrin glue in healing of jaws and facial soft tissues and bone tissues, and in 50% of the cases he achieved earlier wound healing and less scar tissue formation.…”