The Use of SIFT-MS in Profiling the Faecal Volatile Metabolome in Horses with Colic: A Pilot Study

Turner, Claire; Batty, C.; Escalona, Ebony; Hunter, John; Proudman, C. J.

doi:10.2174/15734110113099990026

Cited by 6 publications

(7 citation statements)

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“…Faecal VOCs and VOC profiles have been found to be associated with several intestinal diseases in man and horses [11][12][13][14]. Equinespecific metabolomics studies include a plasma nuclear magnetic resonance (NMR) spectroscopy study using an oligofructose overload model of early time-point laminitis [13] and a study profiling the volatile faecal metabolome of horses with colic [15]. However, there is little data on the factors affecting the EGM of healthy non-thoroughbred horses on standard pasture.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the equine microbiome by pasture and feed supplements: A metabolomics approach

Snalune¹,

Hunter²,

Waring³

et al. 2019

Integr Food Nutr Metab

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Faecal VOCs (volatile organic compounds) were compared in horses and ponies before and after 6 weeks on Spring pasture and with and without a dietary supplement (ERME). The supplement was derived from malted barley and contained enzymes which degrade complex carbohydrates, including amylase, maltase, glucanases and fructanases. VOCs of faecal samples were analysed by selected ion flow tube mass spectrometry (SIFT-MS) and the results showed that the faecal microbiome was altered in different ways both by feeding on Spring pasture and by the dietary supplement. The composition of the equine gut microbiome (EGM) is therefore potentially a reflection of dietary constituents, both pasture and supplements; any metabolomic approach should take this into account since the EGM may have modulating effects on physiological parameters such as diabetes and obesity. Methods Study protocolFaecal samples were collected non-invasively either before and six weeks after supplementation with ERME while keeping the horses/ ponies on pasture for 6 weeks in Spring. The volatile faecal metabolome was characterised using selected ion flow tube-mass spectrometry (SIFT-MS) in conjunction with multivariate data analysis. Study population and samplingA study population of 45 horses and ponies at the Redwings Sanctuary, Norfolk was chosen by the resident veterinary officer to take part in the study. This was performed in Spring from early May to mid-June, the timing being chosen so that the effects of eating new grass could be determined. A power calculation was performed based on previous data using ERME as a dietary supplement [4]. Based on a significance of p<0.05 and a power of 0.8, the sample size required was 18 and a minimum of 20 horses per group was randomly selected. The study population was chosen based on the following inclusion and exclusion criteria. Inclusion criteria included: >2 years old, no physical evidence of disease, based on a clinical examination by a veterinary officer, and resident at the sanctuary for >1 year. Exclusion criteria included being on any medication, pregnancy and no entire males. Horses were randomly allocated to either the treatment group or the control group.Snalune KL (2019) Modulation of the equine microbiome by pasture and feed supplements: A metabolomics approach

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Section: Discussionmentioning

confidence: 99%

Modulation of the equine microbiome by pasture and feed supplements: A metabolomics approach

Snalune¹,

Hunter²,

Waring³

et al. 2019

Integr Food Nutr Metab

View full text Add to dashboard Cite

show abstract

“…Faecal volatile organic compound (VOC) profiling is an economical technique to study the metabolome (functional microbiome). Horses with colic have been shown to have a different faecal VOC profile from healthy horses (Turner et al, 2013). The aim of this study was to characterise the faecal VOC changes in healthy mares pre-and post-partum to understand the normal fluctuations in the metabolome around this time.…”

Section: Temporal Changes In the Faecal Volatile Organic Compound (Vomentioning

confidence: 99%

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Raphaka¹,

Matika²,

Molano³

et al. 2016

Advances in Animal Biosciences

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“…VOC analysis of faeces provides a simple approach to understanding functional changes of the microbiota of the distal intestine. The faecal VOC profiles of horses have been found to alter in response to dietary supplementation, from the ingestion of probiotics and when suffering from gastrointestinal disease (colic) (Turner et al 2013 ; Ishizaka et al 2014 ; Proudman et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

A comparison of sample preparation methods for extracting volatile organic compounds (VOCs) from equine faeces using HS-SPME

2018

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IntroductionDisturbance to the hindgut microbiota can be detrimental to equine health. Metabolomics provides a robust approach to studying the functional aspect of hindgut microorganisms. Sample preparation is an important step towards achieving optimal results in the later stages of analysis. The preparation of samples is unique depending on the technique employed and the sample matrix to be analysed. Gas chromatography mass spectrometry (GCMS) is one of the most widely used platforms for the study of metabolomics and until now an optimised method has not been developed for equine faeces.ObjectivesTo compare a sample preparation method for extracting volatile organic compounds (VOCs) from equine faeces.MethodsVolatile organic compounds were determined by headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GCMS). Factors investigated were the mass of equine faeces, type of SPME fibre coating, vial volume and storage conditions.ResultsThe resultant method was unique to those developed for other species. Aliquots of 1000 or 2000 mg in 10 ml or 20 ml SPME headspace were optimal. From those tested, the extraction of VOCs should ideally be performed using a divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS) SPME fibre. Storage of faeces for up to 12 months at − 80 °C shared a greater percentage of VOCs with a fresh sample than the equivalent stored at − 20 °C.ConclusionsAn optimised method for extracting VOCs from equine faeces using HS-SPME-GCMS has been developed and will act as a standard to enable comparisons between studies. This work has also highlighted storage conditions as an important factor to consider in experimental design for faecal metabolomics studies.Electronic supplementary materialThe online version of this article (10.1007/s11306-017-1315-7) contains supplementary material, which is available to authorized users.

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The Use of SIFT-MS in Profiling the Faecal Volatile Metabolome in Horses with Colic: A Pilot Study

Cited by 6 publications

References 43 publications

Modulation of the equine microbiome by pasture and feed supplements: A metabolomics approach

Modulation of the equine microbiome by pasture and feed supplements: A metabolomics approach

Summaries

A comparison of sample preparation methods for extracting volatile organic compounds (VOCs) from equine faeces using HS-SPME

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