The Use of Semi-Thin Section Method in the Study of Stress-Induced Structural Changes in Lungs

Abstract

The perfect knowledge of the microscopic structure of the appropriate organ is crucial in the solution of many urgent problems of clinical medicine. Better preservation of tissue structures is achieved when the tissues are embedded into epoxy resins followed up with the semi-thin section making. In contrast to the thick sections that are used for light microscopy, due to the greater awareness and relative cost effectiveness without the need of complicated expensive equipment, the method of semithin sections today is currently used in morphological studies. The paper was aimed at the study of the prospects of using the semi-thin section method to evaluate the immobilization stress-induced structural changes in the lungs. The resulting study showed the appropriateness of the use of semi-thin sections to study structural changes in lungs, induced by the experimental stress. The study of the semi-thin sections is the beneficial technique of the morphological analysis that permits to evaluate the structure of the studied material at the tissue and cellular level.

Morphological features of the respiratory part in guinea pigs lung in dynamics of experimental allergic inflammatory process

“…W e demonstrated desquamation of the alveolar epithelium with a violation of the air-blood barrier on the background of a violation of hemomicrocirculatory bed in the early phases of the allergic inflammatory process. Similar changes in the lungs under the influence of various pathological factors were found by other scientists [6,7,11]. The revealed changes can be explained by the rapid degranulation of mastocytes with the release of histamine and leukotrienes, which, in addition to bronchospasm and mucus hyperproduction, caused alterative-exudative changes in the components of the respiratory part of the lungs [1].…”

Morphometric characteristics of distal airways of guinea pigs sensitized with ovalbumin

“…Despite its importance, the problem of studying the morphogenesis of allergic inflammation of the distal airways often remains outside the field of vision of scientists. The number of works, devoted to the study of bronchial morphogenesis in various pathological conditions [9,13], including allergic inflammation in the chronobiological aspect, is insignificant. Considering the above, the study of morphological changes in the distal airways with allergic inflammation in the chronobiological aspect is the urgent issue in experimental medicine.…”