The use of selective adsorbents in capillary electrophoresis-mass spectrometry for analyte preconcentration and microreactions: A powerful three-dimensional tool for multiple chemical and biological applications

Guzman, Norberto A.; Stubbs, R.J.

doi:10.1002/1522-2683(200109)22:17<3602::aid-elps3602>3.0.co;2-x

Cited by 113 publications

(72 citation statements)

References 182 publications

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“…The usefulness of online SPE-CE-MS for quantitative measurements has been shown by Waterval et al [67]. Different materials enable the selective enrichment of analytes from complex matrices [68].…”

Section: Coating and Preconcentrationmentioning

confidence: 99%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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Quantitation in capillary electrophoresis-mass spectrometryCE-MS has evolved into a strong alternative to LC-MS. Most of CE-MS applications deal with characterization and identification. However, quantitative aspects have gained importance in, e.g., pharmaceutical and biotechnological applications. Here we summarize and evaluate various methodological aspects in order to achieve sensitive and reproducible results. Similar to LC-MS, aspects of matrix influence on the electrospray process need to be carefully addressed when quantitative results are intended by CE-MS. Due to a more complicated coupling special emphasis needs to be put on the CE-MS interface. Generally linearity over more than three orders of magnitude can be achieved by CE-ESI-MS. Furthermore, a literature survey has been performed in order to give an overview over quantitative measurements performed by CE-MS. The precision can be doubled when changing from a structural related to an isotopically labeled internal standard. Thus a level of precision better than 5% RSD can be achieved.

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Section: Coating and Preconcentrationmentioning

confidence: 99%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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“…In the field of electrokinetically driven separation, online immunoaffinity CE (IA-CE) has opened the way to more straightforward protocols [1][2][3][4][5]. It involves the online hybridization of two technologies: immunoaffinity extraction and CE.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis immunoassay using magnetic beads

Chen¹,

Busnel²,

Gassner³

et al. 2008

Electrophoresis

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Protein A-coated magnetic beads (0.3 mm) have been trapped in a small portion of a neutrally coated capillary (50 mm id). Anti-b-lactoglobulin (b-LG) antibodies have then been immobilized on the beads through strong affinity with protein A to subsequently capture b-LG from model or real samples. Once the immunocomplexes formed at physiological pH, a discontinuous buffer system has been used to release the partners and preconcentrate them by transient ITP. The antigens and antibodies have finally been separated by CZE and detected by UV absorbance. An LOQ of 55 nM has been achieved. This methodology has been applied to quantify native b-LG in pasteurized and ultra-high-temperature-treated bovine milk. All the described procedures, including immunosorbent preparation, sample extraction, cleanup, preconcentration, and separation are completely automated on a commercial CE instrument. As this CE immunoassay method is simple, rapid, selective, and sensitive, it should be a practical and attractive technology for the analysis of complicated biological samples.

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“…This concept of on-line preconcentration CE has been used as a promising technique to trap, enrich, clean, and analyze a variety of substances present in a cell, organelle, tissue, and/or biological fluid [11,13,14,15]. A two-dimensional separation method, coupling immunoaffinity to CE for the immunoadsorption and separation of a selective immunoreactive substance(s) [11,13,14,15] can be compared to the isolation of a needle from a haystack using a powerful magnet. Antibodies are biological "magnets" that are being used as powerful tools to provide synergy between immunoassay and CE for the development of pharmaceutical, forensic, and clinical applications.…”

Section: Immunoaffinity Capillary Electrophoresis Applications Of CLImentioning

confidence: 99%

Immunoaffinity capillary electrophoresis applications of clinical and pharmaceutical relevance

Guzman

2004

Analytical and Bioanalytical Chemistry

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The primary goal of a clinical chemistry laboratory is to correctly perform analytical procedures that yield accurate and precise information on the constituents of a cell, organelle, tissue, and/or biological fluid to aid in patient diagnosis. Similarly, the primary goal of a drug metabolism laboratory is to correctly perform analytical procedures that yield accurate and precise information on a drug that has been inoculated into a cell culture, an experimental animal, and/or a human being to aid in the understanding of the fate of such a drug (e.g., non-altered intact drug versus a chemically modified drug or metabolite) resulting in cell benefit or toxicity. Routinely, more than 400 common and specialized tests can be performed in a clinical laboratory setting in a single day, and dozens of similar tests can be performed in a drug metabolism laboratory. To accomplish such complex tasks, a variety of methods are currently available, in both types of laboratories, for the determination of a wide range of analytes in simple and complex matrices, including electrophoretic, chromatographic, spectrometric, radiometric, enzymatic, and immunological assays, as well as the measurement of electrolytes by ion-selective electrodes. More recently, capillary electrophoresis (CE) has been added to this battery of traditional tests to determine a large number of substances of small molecular mass, as well as some biomolecules [1,2,3,4,5,6,7,8].Capillary electrophoresis has played a major role in sequencing a number of genomes, providing extensive information on gene sequence, organization, and expression [9, 10]. However, the information gained in the Human Genome Project and many other genome projects is not sufficient to understand the complexity of any living system. The need to study gene products, and the importance in understanding the function of metabolites as active or toxic entities, is becoming the next priority in order to answer questions that genomes alone cannot. In the socalled post-genomic era, proteomics and metabolomics, encompass a group of technologies that attempt to separate, identify, and characterize a global set of proteins and metabolites. A proteome comprises the sequence information for all the proteins present in a cell, organelle, tissue, and/or biological fluid. Moreover, it is beginning to provide information about protein abundance, location, chemical modification, functional activity, interaction networks, and their regulation. Metabolomics comprises the study of the entire metabolite complements in a cell, organelle, tissue, and/or biological fluid. Pharmacokinetic and pharmacodynamic studies are becoming increasingly important in understanding the nature of the interaction of a reactive metabolite, of pharmaceutical and non-pharmaceutical origin, and the various cellular components, as well as the mechanism(s) of action in which these interactions eventually lead to cell benefit or toxicity.Capillary electrophoresis, in capillary format or microchannel format, continues to provide an impre...

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The use of selective adsorbents in capillary electrophoresis-mass spectrometry for analyte preconcentration and microreactions: A powerful three-dimensional tool for multiple chemical and biological applications

Cited by 113 publications

References 182 publications

Quantitation in capillary electrophoresis-mass spectrometry

Quantitation in capillary electrophoresis-mass spectrometry

Capillary electrophoresis immunoassay using magnetic beads

Immunoaffinity capillary electrophoresis applications of clinical and pharmaceutical relevance

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