The use of SDS micelles as additive to increase fluorescence analysis of sitagliptin and saxagliptin derivatives in their tablets and human plasma

Barseem, Aya; Ahmed, Hytham M.; El-Shabrawy, Yasser; Belal, F.

doi:10.1016/j.microc.2018.12.054

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…By comparing this work with others previously reported techniques in literature, including potentiometric sensor [19], Chromatographic [10], spectrophotometric [82] and fluorescence detection [81], as illustrated in Table 3, it is clear that MIP/MwCNT modified CPE sensor has a much lower LOD, higher sensitivity for Saxa, taking into consideration that, this is the first analysis technique relaying on molecular imprinted technology, which is advantageous for its selective recognition ability for its target analyte.…”

Section: Resultsmentioning

confidence: 80%

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

et al. 2023

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Saxagliptin (Saxa) belongs to a new generation of antidiabetic pharmaceutical compounds used in combination with healthy diet and exercise to lower blood glucose levels in patients with type 2 diabetes mellitus (T2DM). In this work, we report for the first time a molecularly imprinted polymer (MIP) based electrochemical sensor for the determination of Saxa. Computational calculations were performed, based on which five MIPs were synthesized using Saxa as a template, itaconic acid as a monomer, crosslinked with ethylene glycol dimethacrylate and Di methyl sulfoxide (DMSO) as a porogen with different ratios. Non‐covalent interaction (NCI) analysis has been also conducted, and the obtained isosurface analysis was used for graphical visualization of NCI that could occur in real space as well as for the discrimination between hydrogen bond interaction, Van Der Waals attraction and spatial repulsion. The optimized polymer was incorporated as a modifier for designing an electrochemical sensor comprising MIP and Multiwalled carbon nanotubes (MwCNT) within carbon paste electrode (CPE). The operational variables including incubation time, pH, scan rate, and accumulation time were optimized. The sensor showed linearity over the concentration range (1 × 10−9–1 × 10−15 M) with low limit of detection (LOD) 8 × 10−16 and 2 × 10−16 M on using DPV and EIS, respectively. The sensor was successfully applied for pharmaceutical formulations, urine, and human serum samples with recovery range between 97.45–100.64 %.

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Section: Resultsmentioning

confidence: 80%

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

et al. 2023

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“…These methods include high performance liquid chromatography (HPLC) [5][6][7], spectrophotometry [2,[8][9][10][11][12] and spectrofluorimetry [13][14][15]. Similarly, SXG was determined using HPLC [16][17][18], spectrophotometric [19][20][21][22][23][24] and spectrofluourimetric [20,22,25] methods. The reported spectrofluorimetric methods for GLZ [13][14][15] and SXG [20,22,25] required prior chemical derivatization reactions using specific reagents; which in turn renders them time consuming and tedious, with a decrease of their greenness profiles.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, SXG was determined using HPLC [16][17][18], spectrophotometric [19][20][21][22][23][24] and spectrofluourimetric [20,22,25] methods. The reported spectrofluorimetric methods for GLZ [13][14][15] and SXG [20,22,25] required prior chemical derivatization reactions using specific reagents; which in turn renders them time consuming and tedious, with a decrease of their greenness profiles. Whereas, in the current study, a green, rapid and simple spectrofluorimetric method was developed for direct estimation of both GLZ and SXG without the need for pre-derivatization steps for the first time.…”

Section: Introductionmentioning

confidence: 99%

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

et al. 2022

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In this study, highly fluorescent sulfur and nitrogen doped carbon quantum dots (S,N-CQDs) were used as fluorescent nanosensors for direct spectrofluorimetric estimation of each of gliclazide (GLZ) and saxagliptin (SXG) without any pre-derivatization steps for the first time. S,N-CQDs were synthesized employing a simple hydrothermal technique using citric acid and thiosemicarbazide. The produced S,N-CQDs were characterized using different techniques including fluorescence emission spectroscopy, UV spectrophotometry, high-resolution transmission electron microscopy and FT-IR spectroscopy. Following excitation at 360 nm, S,N-CQDs exhibited a strong emission peak at 430 nm. The native fluorescence of S,N-CQDs was quantitatively enhanced by addition of increased concentrations of the studied drugs. The fluorescence enhancement of S,N-CQDs and the concentrations of the studied drugs revealed a wide linear relationship in the range of 30.0–500.0 µM and 75.0–600.0 µM with limits of detection of 5.0 and 10.15 µM for GLZ and SXG, respectively. The proposed method was efficiently used for determination of cited drugs in their commercial tablets with % recoveries ranging from 98.6% to 101.2% and low % relative standard deviation values (less than 2%). The mechanism of interaction between S,N-CQDs and the two drugs was studied. Validation of the proposed method was carried out in accordance with International Conference on Harmonization (ICH) guidelines.

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“…Analytical techniques for analyzing sitagliptin in biological media require an understanding of pharmacological processes like distribution, absorption, elimination and metabolism. Various analysis methods for the quantitation of sitagliptin, such as spectrophotometry, [6][7][8][9] spectrofluorimetry, [10,11] HPLC, [12][13][14][15] electrophoresis [16] and voltammetry. [17] Although many of these introduced techniques may be sensitive and accurate, some drawbacks such as low selectivity, sample pretreatment process, time-consuming procedure, special expertise, and expensive and sophisticated devices limit their use in routine analyses.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Chemically Modified Carbon Paste Sensor for Reliable and Selective Potentiometric Determination of Trace Amounts of Sitagliptin in Real Samples

2022

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A new chemically modified potentiometric carbon paste electrode was fabricated based on incorporation of the ion association complex of sitagliptin‐phosphotungstate to determine sitagliptin. The proposed sensor displayed a Nernstian slope of 59.4 mV/decade for sitagliptin over a concentration range of 1.7×10−8 to 2.2×10−5 M and a detection limit of 9.1×10−9 M. It represented the advantages of fast response, long lifetime and, most importantly, excellent selectivity for sitagliptin versus a wide variety of usual inorganic cations and also biological species such as amino acids and sugars. The temperature dependence of sensor potential was examined, and it was figured out that the sensor has a low thermal coefficient in the temperature range of 15–55 °C. The sensor was applied to determine sitagliptin in pharmaceutical tablets, milk and blood serum samples. The obtained suitable recoveries and relative standard deviations confirmed that the developed sensor could be precisely applied to determine sitagliptin in real samples.

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The use of SDS micelles as additive to increase fluorescence analysis of sitagliptin and saxagliptin derivatives in their tablets and human plasma

Cited by 14 publications

References 17 publications

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

Design and application of molecularly imprinted electrochemical sensor for the new generation antidiabetic drug saxagliptin

Application of sulfur and nitrogen doped carbon quantum dots as sensitive fluorescent nanosensors for the determination of saxagliptin and gliclazide

Ultrasensitive Chemically Modified Carbon Paste Sensor for Reliable and Selective Potentiometric Determination of Trace Amounts of Sitagliptin in Real Samples

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