The use of rhBMP2 for augmentation of established horizontal/vertical defects may require additional use of rhVEGF to achieve significant bone regeneration: An in vivo experimental study

Kauffmann, Philipp; Raschke, David; Tröltzsch, Markus; Santander, Petra; Brockmeyer, Phillipp; Schliephake, Henning

doi:10.1111/clr.13820

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…This indicates an early hampering effect of the scaffolds themselves on bone regeneration. Kauffmann and coworkers observed a similar effect for particles made of PDLLA/CaCO 3 that were implanted into 3-monthold, not already healed bone defects in the alveolar crest of the maxillae of minipigs [39]. After 4 weeks of healing, PDLLA/CaCO 3 -treated animals showed less newly formed bone compared to SHAM-operated animals, with no signs of the induction of bone formation between the PDLLA/CaCO 3 particles that were furthermore separated from the underlying native bone by a fibrous tissue layer.…”

Section: Discussionmentioning

confidence: 72%

Poly(dl-lactide) Polymer Blended with Mineral Phases for Extrusion 3D Printing—Studies on Degradation and Biocompatibility

Vater,

Bräuer,

Grom

et al. 2024

Polymers

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A promising therapeutic option for the treatment of critical-size mandibular defects is the implantation of biodegradable, porous structures that are produced patient-specifically by using additive manufacturing techniques. In this work, degradable poly(DL-lactide) polymer (PDLLA) was blended with different mineral phases with the aim of buffering its acidic degradation products, which can cause inflammation and stimulate bone regeneration. Microparticles of CaCO3, SrCO3, tricalcium phosphates (α-TCP, β-TCP), or strontium-modified hydroxyapatite (SrHAp) were mixed with the polymer powder following processing the blends into scaffolds with the Arburg Plastic Freeforming 3D-printing method. An in vitro degradation study over 24 weeks revealed a buffer effect for all mineral phases, with the buffering capacity of CaCO3 and SrCO3 being the highest. Analysis of conductivity, swelling, microstructure, viscosity, and glass transition temperature evidenced that the mineral phases influence the degradation behavior of the scaffolds. Cytocompatibility of all polymer blends was proven in cell experiments with SaOS-2 cells. Patient-specific implants consisting of PDLLA + CaCO3, which were tested in a pilot in vivo study in a segmental mandibular defect in minipigs, exhibited strong swelling. Based on these results, an in vitro swelling prediction model was developed that simulates the conditions of anisotropic swelling after implantation.

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Section: Discussionmentioning

confidence: 72%

Poly(dl-lactide) Polymer Blended with Mineral Phases for Extrusion 3D Printing—Studies on Degradation and Biocompatibility

Vater,

Bräuer,

Grom

et al. 2024

Polymers

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“…Six studies reported chronic type BD in the mandibular ( n = 5; 2–4.5 cm long buccal defects) (Duong et al., 2023; Herford et al., 2012; Stricker et al., 2014; Yeo et al., 2012; Zambon et al., 2012) or maxillary alveolar ridge ( n = 1; 2 cm long buccal defect) (Kauffmann et al., 2021). In all studies, tooth extraction and BD were performed in a preliminary surgery followed by a healing period (4–12 weeks) to allow ‘chronification’ (mimicking atrophic ridges) before application of the regenerative procedure.…”

Section: Resultsmentioning

confidence: 99%

Regeneration of alveolar bone defects in the experimental pig model: A systematic review and meta‐analysis

Shanbhag,

Kampleitner,

Sanz‐Esporrin

et al. 2024

Clinical Oral Implants Res

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ObjectivePigs are emerging as a preferred experimental in vivo model for bone regeneration. The study objective was to answer the focused PEO question: in the pig model (P), what is the capacity of experimental alveolar bone defects (E) for spontaneous regeneration in terms of new bone formation (O)?MethodsFollowing PRISMA guidelines, electronic databases were searched for studies reporting experimental bone defects or extraction socket healing in the maxillae or mandibles of pigs. The main inclusion criteria were the presence of a control group of untreated defects/sockets and the assessment of regeneration via 3D tomography [radiographic defect fill (RDF)] or 2D histomorphometry [new bone formation (NBF)]. Random effects meta‐analyses were performed for the outcomes RDF and NBF.ResultsOverall, 45 studies were included reporting on alveolar bone defects or extraction sockets, most frequently in the mandibles of minipigs. Based on morphology, defects were broadly classified as ‘box‐defects’ (BD) or ‘cylinder‐defects’ (CD) with a wide range of healing times (10 days to 52 weeks). Meta‐analyses revealed pooled estimates (with 95% confidence intervals) of 50% RDF (36.87%–63.15%) and 43.74% NBF (30.47%–57%) in BD, and 44% RDF (16.48%–71.61%) and 39.67% NBF (31.53%–47.81%) in CD, which were similar to estimates of socket‐healing [48.74% RDF (40.35%–57.13%) and 38.73% NBF (28.57%–48.89%)]. Heterogeneity in the meta‐analysis was high (I2 > 90%).ConclusionA substantial body of literature revealed a high capacity for spontaneous regeneration in experimental alveolar bone defects of (mini)pigs, which should be considered in future studies of bone regeneration in this animal model.

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“…Table 3 shows several cytokines loaded with bone biomaterials that promote angiogenesis and osteogenesis. Among all these factors, VEGF is the most commonly used in bone tissue engineering (Li et al, 2020b;Kauffmann et al, 2021).…”

Section: The Effect Of Cytokine-loaded Bone Bioscaffolds On Angiogenesismentioning

confidence: 99%

Research progress of vascularization strategies of tissue-engineered bone

Lv,

Zhou,

Hou

et al. 2024

Front. Bioeng. Biotechnol.

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The bone defect caused by fracture, bone tumor, infection, and other causes is not only a problematic point in clinical treatment but also one of the hot issues in current research. The development of bone tissue engineering provides a new way to repair bone defects. Many animal experimental and rising clinical application studies have shown their excellent application prospects. The construction of rapid vascularization of tissue-engineered bone is the main bottleneck and critical factor in repairing bone defects. The rapid establishment of vascular networks early after biomaterial implantation can provide sufficient nutrients and transport metabolites. If the slow formation of the local vascular network results in a lack of blood supply, the osteogenesis process will be delayed or even unable to form new bone. The researchers modified the scaffold material by changing the physical and chemical properties of the scaffold material, loading the growth factor sustained release system, and combining it with trace elements so that it can promote early angiogenesis in the process of induced bone regeneration, which is beneficial to the whole process of bone regeneration. This article reviews the local vascular microenvironment in the process of bone defect repair and the current methods of improving scaffold materials and promoting vascularization.

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The use of rhBMP2 for augmentation of established horizontal/vertical defects may require additional use of rhVEGF to achieve significant bone regeneration: An in vivo experimental study

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 5 publications

References 34 publications

Poly(dl-lactide) Polymer Blended with Mineral Phases for Extrusion 3D Printing—Studies on Degradation and Biocompatibility

Poly(dl-lactide) Polymer Blended with Mineral Phases for Extrusion 3D Printing—Studies on Degradation and Biocompatibility

Regeneration of alveolar bone defects in the experimental pig model: A systematic review and meta‐analysis

Research progress of vascularization strategies of tissue-engineered bone

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