The use of radio frequency sputter ion etching and scanning electron microscopy to study the internal structure of biological material

Stuart, P. R.; Osborn, J S; Lewis, S. M.

doi:10.1016/s0042-207x(69)91840-5

Cited by 14 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In ion etching, ions are extracted from a glow discharge in an inert gas at low pressure and are attracted to the target surface, from which about one atom is typically knocked off by each ion-surface collision (Stuart et al, 1969). This selective removal of substrate atoms makes structural features within materials observable by scanning electron microscopy.…”

Section: Discussionmentioning

confidence: 99%

“…This selective removal of substrate atoms makes structural features within materials observable by scanning electron microscopy. The resistance of material components to removal by argon-ion-beam etching is related to the composition and structure ofthe material (Boyde and Stewart, 1962;Stuart et al, 1969;Vahl et al, 1972). The argon-ion bombardment method presently used for disclosing the interface between the adhesive resin and the conditioned and/or primed dentin surface was first introduced by Inokoshi et al (1990).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological Aspects of the Resin-Dentin Interdiffusion Zone with Different Dentin Adhesive Systems

Meerbeek

Inokoshi

Braem³

et al. 1992

J Dent Res

463

217

View full text Add to dashboard Cite

Cross-sections of resin-dentin interfaces were etched with an argon-ion beam to make their substructure detectable by scanning electron microscopy. The dentin adhesive systems were categorized morphologically into three groups, and an attempt was made to clarify their adhesive mechanism. The first group of products removed the smear layer. The argon-ion bombardment clearly disclosed a hybrid or resin-impregnated dentin layer. It is hypothesized that conditioning with acidic or chelating agents demineralized the dentin surface-layer to a certain depth, leaving behind a collagen-rich mesh-work. Hydrophilic monomers are then believed to alter this collagen-fiber arrangement in a way that facilitates penetration of the adhesive resin, resulting in a mechanical, intermingled link between collagen and the adhesive resin. The second group preserved the smear layer. In this case, the dentinal tubules were obliterated with globular particles at their orifices and remained patent underneath these smear plugs. This type of adhesive system aims at the incorporation of the smear layer into the hydrophilic monomers, which have an affinity for the organic and/or inorganic components of the underlying dentin. Finally, a third, small group only partly dissolved the smear layer, creating a thin resin-impregnated dentin layer and a resin-impregnated smear plug. This study clearly showed that the application of recent adhesive systems induced structural changes in the dentin surface morphology, creating a retentive interface, called the inter-diffusion zone, between the deep, untouched dentin layers and the composite filling material. This resin-dentin interdiffusion zone offers bonding sites for copolymerization with the resin composite and, concurrently, might have protective potential for the pulp tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Morphological Aspects of the Resin-Dentin Interdiffusion Zone with Different Dentin Adhesive Systems

Meerbeek

Inokoshi

Braem³

et al. 1992

J Dent Res

463

217

View full text Add to dashboard Cite

show abstract

“…Stuart et al (1969) and Lewis et at. (1968) examined human erythrocytes after etching with hydrogen, oxygen, argon or nitrogen.…”

Section: Introductionmentioning

confidence: 93%

Internal Structure of the Chicken Erythrocyte after Exposure to Hydrogen Plasma as Seen With Sem

Bayer

1975

Poultry Science

View full text Add to dashboard Cite

show abstract

“…Most frequently however, etching is carried out with argon ions [4,9]. Under this approach the test surface is bombarded with argon ions, whereupon an atom is removed from the surface, typically through collision with an argon ion [10]. As argon etching is effective in a substratum-specific manner, structures of varying composition are removed to varying degrees by means of this procedure, which, on subsequent observation under a SEM, allows a differentiated image of the adhesive interface as compared with an unetched sample [6,11].…”

Section: Introductionmentioning

confidence: 99%

The ultrastructure of a compomer adhesive interface in enamel and dentin, and its marginal adaptation under dentinal fluid as compared to that of a composite

et al. 1999

View full text Add to dashboard Cite

Objectives: To visualise the ultrastructure of the interface of SCA compomer adhesive and of Optibond composite adhesive in enamel and dentin, and to relate the findings to the marginal adaptation of these two products in mixed class V restorations.Methods: The ultrastructure was investigated using a scanning electron microscope (SEM) with and without prior argon ion etching, an environmental SEM, a field emission SEM, a confocal laser scanning microscope, and a transmission electron microscope. The marginal adaptation was quantified in mixed class V restorations by using the replica technique and a SEM under simulated dentinal fluid before and after simultaneous mechanical and thermal loading.Results: The ultrastructure of the compomer adhesive interface differed from those of the composite. However, no significant difference was discerned as regards the percentage of "continuous margin" in the enamel marginal area before loading, and in the dentin area before and after loading (p Ͻ 0:05; unpaired t-test). Only after loading, the percentage of "continuous margin" in enamel was significantly (p Ͻ 0:05; unpaired t-test) better than that of the compomer.Significance: The results indicated that the ultrastructure of the adhesive interface allowed no clear conclusions to be drawn as to the quality of marginal adaptation. ᭧

show abstract

The use of radio frequency sputter ion etching and scanning electron microscopy to study the internal structure of biological material

Cited by 14 publications

References 9 publications

Morphological Aspects of the Resin-Dentin Interdiffusion Zone with Different Dentin Adhesive Systems

Morphological Aspects of the Resin-Dentin Interdiffusion Zone with Different Dentin Adhesive Systems

Internal Structure of the Chicken Erythrocyte after Exposure to Hydrogen Plasma as Seen With Sem

The ultrastructure of a compomer adhesive interface in enamel and dentin, and its marginal adaptation under dentinal fluid as compared to that of a composite

Contact Info

Product

Resources

About