The use of pore-forming toxins to image lipids and lipid domains

Tomishige, Nario; Murate, Motohide; Didier, Pascal; Richert, Ludovic; Kobayashi, Toshihide

doi:10.1016/bs.mie.2021.01.019

Cited by 14 publications

(9 citation statements)

References 67 publications

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“…The E69A and E69S mutants of OlyA can bind to both free sphingomyelin and sphingomyelin complexed with CHOL and thus could be used as sphingomyelin probes (another powerful sphingomyelin probe is lysenin [ Yamaji et al. , 1998 ; Tomishige et al. , 2021 ]).…”

Section: Chol-centered View Of the Pmmentioning

confidence: 99%

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†

Kusumi

Tsunoyama

Tang

et al. 2023

MBoC

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Two very polarized views exist for understanding the cellular plasma membrane (PM). For some, it is the simple fluid described by the original Singer–Nicolson fluid mosaic model. For others, due to the presence of thousands of molecular species that extensively interact with each other, the PM forms various clusters and domains that are constantly changing and therefore, no simple rules exist that can explain the structure and molecular dynamics of the PM. In this article, we propose that viewing the PM from its two predominant components, cholesterol and actin filaments, provides an excellent and transparent perspective of PM organization, dynamics, and mechanisms for its functions. We focus on the actin-induced membrane compartmentalization and lipid raft domains coexisting in the PM and how they interact with each other to perform PM functions. This view provides an important update of the fluid mosaic model.

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Section: Chol-centered View Of the Pmmentioning

confidence: 99%

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†

Kusumi

Tsunoyama

Tang

et al. 2023

MBoC

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“…The SM localization was studied by using the SM probe, enhanced green fluorescent protein-nontoxiclysenin (EGFP-NT-Lys). The probe was purified from E.coli strain BL21(DE3) harboring pET28/EGFP-NT-Lys according to Tomishige et al [40], with a little modification. In brief, after bacteria culture reaches OD600 = approx.…”

Section: Immunofluorescencementioning

confidence: 99%

Vitamin C Modifies the Phenotype of Hormone Receptor Positive/HER2 Negative Breast Cancer Cells and Induces Changes in Sphingolipid Metabolism

Codini¹,

Fiorani²,

Mandarano³

et al. 2023

Preprint

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The effect of the antioxidant vitamin C in cancer has been studied for over 30 years with conflict-ing results ranging from total absence of effect, to possible protective effect, to potential therapeutic effect when administered in high doses. The aim of the study was to define the effect of vitamin C in breast cancer and the possible involvement of sphingomyelin metabolism. Therefore, the effect of low and high dose of vitamin C on cells reproducing luminal A type (MCF7) and triple negative type (MB231) of breast cancer was analyzed. The results showed that high dose of vitamin C produced effects only in MCF7 cells such as decreased cell viability, modulation of cell cycle re-lated genes, unexpected change in cell phenotype due to estrogen receptor downregulation, catabo-lism of sphingomyelin with a large increase in ceramide. In conclusion, we demonstrated that high doses of VitC on the one hand reduce the growth of lu-minal A type breast cancer cells and on the other hand induce their phenotypic change towards hormone therapy resistant cells. The effect of vitamin C on therapeutic treatment failure of luminal A breast cancer and the possible involvement of high ceramide levels were discussed.

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“…The SM localization was studied by using the SM probe, enhanced green fluorescent protein-nontoxiclysenin (EGFP-NT-Lys). The probe was purified from E.coli strain BL21(DE3) harboring pET28/EGFP-NT-Lys according to Tomishige et al [20], with a little modification. In brief, after bacteria culture reaches OD 600 = approx.…”

Section: Fluorescencementioning

confidence: 99%

Sphingomyelin in Human Breast Milk might be Essential for the Hippocampus Maturation

Albi¹,

Arcuri²,

Kobayashi³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: It has been established that sphingomyelin present human breast milk is useful for the brain maturation and cognitive development. At 10 days of breastfeeding the sphingomyelin content is double that present in cow's milk and its content is independent of the maternal diet. The aim of the study was to analyze the content of sphingomyelin in breast milk at 3 months of breastfeeding and to consider the effect of this molecule on synaptic function and nerve conduction through the probable expansion of myelinated axons. Methods: Therefore, to begin to define and assess this, we performed sphingolipidomic analysis in human breast milk. Then, we cultured embryonic hippocampal cells (HN9.10) in the presence of sphingomyelin at a concentration from 0.6% to 31% of human milk, estimated by considering its bioavailability and its passage into the interstitial fluid. To highlight the effect of sphingomyelin in the cells, cell viability and morphology were evaluated. Analyses of neutral sphingomyelinase gene and protein expression was performed. The entry of sphingomyelin into the cell was studied in immunofluorescence; the expression of heavy neurofilament (NF200) was tested with immunocytochemical technique. Results: We demonstrated that sphingomyelin is able to enter cell nucleus and overexpress the sphingomyelin phosphodiesterase 4 (SMPD4) gene encoding for neutral sphingomyelinase (nSMase), an enzyme useful for its own metabolism. Later, cells displayed changes of the soma and the appearance of neurites supported by NF200 overexpression. Conclusions: We speculated that the sphingomyelin present in human breast milk is useful in part to regulate nuclear activity and in part to form myelin sheet to facilitate nerve cell maturation. As brain development occurs at 0-3 years, these data open a new avenue of potential intervention to integrate the infant formulas with SM to obtain a product similar to the maternal milk.

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The use of pore-forming toxins to image lipids and lipid domains

Cited by 14 publications

References 67 publications

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†

Vitamin C Modifies the Phenotype of Hormone Receptor Positive/HER2 Negative Breast Cancer Cells and Induces Changes in Sphingolipid Metabolism

Sphingomyelin in Human Breast Milk might be Essential for the Hippocampus Maturation

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The use of pore-forming toxins to image lipids and lipid domains

Cited by 14 publications

References 67 publications

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary†

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary†

Vitamin C Modifies the Phenotype of Hormone Receptor Positive/HER2 Negative Breast Cancer Cells and Induces Changes in Sphingolipid Metabolism

Sphingomyelin in Human Breast Milk might be Essential for the Hippocampus Maturation

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Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†

Cholesterol- and actin-centered view of the plasma membrane: updating the Singer–Nicolson fluid mosaic model to commemorate its 50th anniversary^†