The use of polyethylenimine-grafted graphene nanoribbon for cellular delivery of locked nucleic acid modified molecular beacon for recognition of microRNA

Dong, Haifeng; Ding, Lin; Yan, Feng; Ji, Hanxu; Ju, Huangxian

doi:10.1016/j.biomaterials.2011.02.001

Cited by 209 publications

(130 citation statements)

References 45 publications

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“…The most common approach consists of the covalent engraftment of polyethylanimine (PEI) via EDC/ NHS chemistry onto both GO and reduced GO (rGO) flakes 34,[37][38][39][40][41][42][43][44][45][46][47] . Noncovalent but electrostatic interactions have also been used to anchor PEI onto graphene nanoribbons (GNR) 48 , GO 49 and rGO/Au composites 50 . Thanks to the above properties, PEI has been used as a non-viral gene delivery vector on its own, however compromised by its cytotoxicity, especially at high molecular weight (25 kDa) and high nitrogen-to-phosphate ratios 51 .…”

Section: Strategies To Optimize Gbms As Gene Delivery Platformmentioning

confidence: 99%

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Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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Section: Strategies To Optimize Gbms As Gene Delivery Platformmentioning

confidence: 99%

“…In a different study, Dong et al similarly developed a method to detect microRNAs in single cells 48 . In this case, the delivery of a MB with high affinity for miR-21 proved more efficient when complexed to a PEI-GNR in comparison to other vectors such as PEI alone and PEIMWCNTs.…”

Section: Intracellular Molecular Sensingmentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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“…Some references revealed that hydrophilic GO is able to penetrate cell membranes, resulting in cytoplasmatic, perinuclear and nuclear accumulation [118][119][120]; while this behavior of GO can be inhibited by chemical or physical functionalization with polymers [120]. Furthermore, GO increases the surface hydrophilicity of the biocomposites, leading to improved biocompatibility [121].…”

Section: Biological Property Of Go-based Materialsmentioning

confidence: 99%

The Preparation of Graphene Oxide and Its Derivatives and Their Application in Bio-Tribological Systems

et al. 2014

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Graphene oxide (GO) can be readily modified for particular applications due to the existence of abundant oxygen-containing functional groups. Graphene oxide-based materials (GOBMs), which are biocompatible and hydrophilic, have wide potential applications in biomedical engineering and biotechnology. In this review, the preparation and characterization of GO and its derivatives are discussed at first. Subsequently, the biocompatibility and tribological behavior of GOBMs are reviewed. Finally, the applications of GOBMs as lubricants in bio-tribological systems are discussed in detail.

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“…50 Apart from mRNA and biomolecule sensing, PEI grafted graphene nanoribbons (PEI-GNR) loaded with MB were also used as molecular probes by Dong et al for sensing of miR-21 in cells. 51 The PEI-GNR showed more e±cient delivery of MB as compared to PEI-MWCNTs and PEI alone. In a follow up study by the same group, the authors developed a multifunctional poly (L-lactide)-Polyethylene glycol-grafted graphene quantum dot-based platform for intracellular sensing of miR-21 combined with°uorescent tracking of the internalization of the complexes using GQDs.…”

Section: Graphene-based Nanomaterials For Nucleic Acid Deliverymentioning

confidence: 96%

Graphene-Based Nanomaterials for Theranostic Applications

Roy

Jaiswal

2017

Rep. Adv. Phys. Sci.

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Graphene and graphene-based nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) have gained a lot of attention from diverse scienti¯c¯elds for applications in sensing, catalysis, nanoelectronics, material engineering, energy storage and biomedicine due to its unique structural, optical, electrical and mechanical properties. Graphene-based nanomaterials emerge as a novel class of nanomedicine for cancer therapy for several reasons. Firstly, its structural properties like high surface area and aromaticity enables easy loading of hydrophobic drugs. Secondly, presence of oxygen containing functional groups improve its physiological stability and also act as site for biofunctionalization. Thirdly, its optical absorption in the NIR region enable them to act as photoagents for photothermal and photodynamic therapies of cancer, both in vitro and in vivo. Finally, its intrinsic°u orescence property helps in bioimaging of cancer cells. Overall, graphene-based nanomaterials can act as agents for developing multifunctional theranostic platforms for carrying out more e±cient detection and treatment of cancers. This review provides a detailed summary of the di®erent applications of graphene-based nanomaterials in drug delivery, nucleic acid delivery, phototherapy, bioimaging and theranostics.

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The use of polyethylenimine-grafted graphene nanoribbon for cellular delivery of locked nucleic acid modified molecular beacon for recognition of microRNA

Cited by 209 publications

References 45 publications

Graphene materials as 2D non-viral gene transfer vector platforms

Graphene materials as 2D non-viral gene transfer vector platforms

The Preparation of Graphene Oxide and Its Derivatives and Their Application in Bio-Tribological Systems

Graphene-Based Nanomaterials for Theranostic Applications

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