The use of platelet density and volume measurements to estimate the severity of pre‐eclampsia

Järemo, Petter; Lindahl, Tomas; Lennmarken, C.; Forsgren, Håkan

doi:10.1046/j.1365-2362.2000.00753.x

Cited by 87 publications

(73 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Impaired platelet activation, both in the form of decreased platelet count and increased MPV, were associated with severity of HT in patients with PE in our study. Similar to our observations, Dundar et al and Jaremo et al reported a signifi cant relationship between MPV and PE (7,8) . Although the rapid turnover of platelets has been put forward as an explanation for this decrease in platelet count and increase in MPV, that study had a small sample size and did not investigate NLR.…”

Section: Discussionsupporting

confidence: 92%

“…Despite previous studies showing an association between hematological parameters and PE, the relationship between the severity of hypertension and hematologic parameters in preeclamptic women has not been thoroughly studied (7,8) . This study was undertaken to investigate the relationship between the severity of hypertension (HT) and hematologic parameters in preeclamptic women that can be measured by a simple complete blood count.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mean Platelet Volume and Neutrophil Lymphocyte Ratio as New Markers of Preeclampsia Severity

Akıl¹

2015

Kosuyolu Heart Journal

View full text Add to dashboard Cite

Introduction: This study was undertaken to examine the role of hematological parameters in predicting the severity of hypertension in pre-eclamptic women. Patients and Methods:Two hundred sixty-one pre-eclamptic women and fi fty-one women with normal pregnancy were included in the study. The severity of preeclampsia was classifi ed as mild or severe.Results: Compared to post-delivery period, pre-eclamptic females had lower platelet count, raised mean platelet volume and raised neutrophil lymphocyte ratio (p< 0.001, p< 0.001, p< 0.001, respectively). Multiple linear regression analysis showed an independent correlation between the severity of hypertension and mean platelet volume as well as the neutrophil lymphocyte ratio (β= 0.25, p< 0.001; β= 0.31, p< 0.001, respectively). Conclusion:Both mean platelet volume and neutrophil lymphocyte ratio emerged as independent predictors of the severity of hypertension in preeclampsia.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Mean Platelet Volume and Neutrophil Lymphocyte Ratio as New Markers of Preeclampsia Severity

Akıl¹

2015

Kosuyolu Heart Journal

View full text Add to dashboard Cite

show abstract

“…The previous data on circulating MPO levels in patients with preeclampsia were conflicting 28 ; our data agree with studies reporting increased MPO in the circulation of women with preeclampsia. 1,29 Unlike the placenta, circulating MPO levels did not significantly increase with gestation. The most plausible source of MPO in the circulation is activated inflammatory cells in either the circulation or tissue.…”

Section: Discussionmentioning

confidence: 84%

“…Preeclampsia is associated with increased neutrophil counts. 1 Peripheral blood leukocytes are activated in women with normal third trimester pregnancies compared with nonpregnant patients. Leukocytes in normal pregnancy have increased markers of activation (CD11b, CD14, CD64, and intracellular reactive oxygen species), which are further increased in preeclampsia.…”

mentioning

confidence: 99%

Increased Myeloperoxidase in the Placenta and Circulation of Women With Preeclampsia

et al. 2008

View full text Add to dashboard Cite

Abstract-Myeloperoxidase (MPO) is a hemoprotein normally released from activated monocytes and neutrophils.Traditionally viewed as a microbicidal enzyme, MPO also induces low-density lipoprotein oxidation, activates metalloproteinases, and oxidatively consumes endothelium-derived NO. The elevated plasma MPO level is a risk factor for myocardial events in patients with coronary artery disease. Patients with preeclampsia display evidence of the inflammation and endothelial dysfunction associated with oxidative stress in the circulation, vasculature, and placenta. We hypothesized that MPO levels in the circulation and placental extracts from women with preeclampsia would be greater than levels in women with normal pregnancies. Placental extracts were prepared from placental villous biopsies from preeclamptic (nϭ27) and control (nϭ43) placentas. EDTA plasma samples were obtained from gestationally age-matched preeclamptic and control normal pregnancies. MPO concentrations were measured by ELISA. Immunohistochemistry was used to determine MPO localization in the placenta. MPO levels in placental extracts from women with preeclampsia were significantly higher than the levels in normal control subjects (546Ϯ62 versus 347Ϯ32 ng/mL; Pϭ0.025). MPO was found in the floating villi and basal plate of placentas with a greater staining in the basal plates from preeclampsia placentas compared with normal pregnancies. Plasma MPO levels were 3-fold higher in patients with preeclampsia compared with normal control subjects (36.6Ϯ7.6 versus 11.0Ϯ3.1 ng/mL; Pϭ0.003). In conclusion, MPO levels are significantly increased in the circulation and placenta of women with preeclampsia. We speculate that MPO may contribute to the oxidative damage reported in the endothelium and placenta of women with preeclampsia.

show abstract

“…Due to a technical failure, platelet reactivity, as judged from the platelet fibrinogen binding after agonist provocation, was not analysed in the healthy control group. An enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MN, USA) was used to analyse soluble P-selectin [35]. In order to avoid platelet activation during blood processing, a platelet inhibitory mixture was used as anticoagulant.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Platelets and acute cerebral infarction

et al. 2012

Self Cite

View full text Add to dashboard Cite

Stroke is worldwide a leading cause of death and disability. Its etiology is regarded as heterogeneous. Platelets are implicated in its pathophysiology, but our understanding of their specific role is incomplete. Only sparse and conflicting information exists about platelet reactivity and activity in acute stroke. Some scientists take the view that platelets activate in conjunction with acute cerebral infarctions. Others put forward evidence corroborating the contrary notion. Increased soluble P-selectin as a sign of platelet and/or endothelial activity seems to be a feature of the disease. The latter point of view is opposed by other researchers. Due to these conflicting opinions, this study is devoted to platelet characteristics in acute cerebral infarctions. We studied subjects (n ¼ 72; age 74 AE 10(SD) years; 31 females) having acute stroke. As controls served atrial fibrillation (AF) patients (n ¼ 58; age 69 AE 7(SD) years; 12 females) subject to electrical cardioversion, a flow cytometer was put to use for measuring platelet reactivity and activity. After agonist provocation, both platelet bound P-selectin and fibrinogen were employed as estimates of platelet reactivity. Dilutions of a thrombin-receptor-activating peptide (TRAP-6) (74 and 57 mmol/l) (P-selectin and fibrinogen) and ADP (8.5 and 1.7 mmol/l) (fibrinogen only) were put to use as platelet agonists. Membrane-bound P-selectin without agonist stimulation served as a measure of in vivo platelet activation. Soluble P-selectin, as determined from a commercial ELISA, was used to assess platelet and/or endothelial activity. In acute stroke neither platelet-bound P-selectin nor fibrinogen after stimulation, i.e. reactivity, differed from AF controls. In contrast, lower platelet activity as judged from surface attached and circulating P-selectin without agonist stimulation proved to be a feature of cerebral infarctions. The p-values were p < 0.001 and p < 0.01, respectively. It is concluded that acute stroke is not associated with platelet reactivity platelets circulate less activated during the disease. It is evident that the mechanisms reflecting platelet reactivity and activity being investigated in this study play minor roles in stroke pathophysiology. New powerful platelet inhibitory drugs are currently introduced. To avoid major bleeding studies on platelet, behavior in acute stroke are necessary before including these medications in stroke treatment protocols.

show abstract

The use of platelet density and volume measurements to estimate the severity of pre‐eclampsia

Cited by 87 publications

References 16 publications

Mean Platelet Volume and Neutrophil Lymphocyte Ratio as New Markers of Preeclampsia Severity

Mean Platelet Volume and Neutrophil Lymphocyte Ratio as New Markers of Preeclampsia Severity

Increased Myeloperoxidase in the Placenta and Circulation of Women With Preeclampsia

Platelets and acute cerebral infarction

Contact Info

Product

Resources

About