The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases

Wachsmuth, I K; Kiehlbauch, Julia A.; Bopp, Cheryl A.; Cameron, Daniel N.; Strockbine, Nancy; Wells, JoyG; Blake, Paul A.

doi:10.1016/0168-1605(91)90049-u

Cited by 55 publications

(21 citation statements)

References 31 publications

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“…Our results with the E3CJC2 probe, using a limited panel of strains, also found variability within these groups, particularly amongst strains of HS 1 (Table 2a). No genotype variation was detected amongst the HS 2/HL 4 strains examined in this study, contrary to the variability reported elsewhere [2,3,21]. However, our analysis is restricted to strains showing the defined stable antigenic associations predominating in human infections (e.g.…”

Section: Discussioncontrasting

confidence: 85%

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

et al. 1996

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SUMMARYCampylobacter jejuni serogroup reference strains and collections of sporadic and outbreakassociated isolates were examined for restriction fragment length polymorphisms (RFLPs), using C. jejuni random chromosomal and 16S rRNA gene probes. A collection of 48 Penner (HS) and 14 Lior (HL) serogroup reference strains, plus 10 clinical isolates, generated 35 RFLP and 26 ribotype patterns. In combination the two loci generated 48 distinct genotypes. Both probes were able to differentiate between certain random isolates of the same HS/HL serogroups but greater discrimination was obtained with RFLP than with ribotyping.

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Section: Discussioncontrasting

confidence: 85%

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

et al. 1996

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“…Subtyping for surveillance and investigation of food-borne disease outbreaks attributable to Salmonella serotype Enteritidis, however, has been hampered by the fact that Salmonella serotype Enteritidis is one of the most genetically homogenous serotypes of Salmonella and is poorly differentiated by the most commonly used subtyping methods. Phage typing (PT) is a classical method traditionally used for subtype determination of Salmonella serotype Enteritidis but has limited discriminatory power and requires specialized phage collections that are available to only a few reference laboratories (20,38,39,49). Plasmid profiling, single-enzyme ribotyping, and random amplified polymorphic DNA (RAPD) analysis also have limited discriminatory power for Salmonella serotype Enteritidis and, in addition, suffer from poor reproducibility (14,20,21,27,38,39,49).…”

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confidence: 99%

“…Phage typing (PT) is a classical method traditionally used for subtype determination of Salmonella serotype Enteritidis but has limited discriminatory power and requires specialized phage collections that are available to only a few reference laboratories (20,38,39,49). Plasmid profiling, single-enzyme ribotyping, and random amplified polymorphic DNA (RAPD) analysis also have limited discriminatory power for Salmonella serotype Enteritidis and, in addition, suffer from poor reproducibility (14,20,21,27,38,39,49). Two-enzyme ribotyping (PstI-SphI) may provide higher discriminatory power than other methods (6); however, manual ribotyping is labor-intensive while automated ribotyping is prohibitively expensive.…”

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confidence: 99%

Comparison of Multiple-Locus Variable-Number Tandem Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Phage Typing for Subtype Analysis of Salmonella enterica Serotype Enteritidis

et al. 2007

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Strain subtyping is an important tool for detection of outbreaks caused by Salmonella enterica serotype Enteritidis. Current subtyping methods, however, yield less than optimal subtype discrimination. In this study, we describe the development and evaluation of a multiple-locus variable-number tandem repeat analysis (MLVA) method for subtyping Salmonella serotype Enteritidis. The discrimination ability and epidemiological concordance of MLVA were compared with those of pulsed-field gel electrophoresis (PFGE) and phage typing. MLVA provided greater discrimination among non-epidemiologically linked isolates than did PFGE or phage typing. Epidemiologic concordance was evaluated by typing 40 isolates from four food-borne disease outbreaks. MLVA, PFGE, and, to a lesser extent, phage typing exhibited consistent subtypes within an outbreak. MLVA was better able to differentiate isolates between the individual outbreaks than either PFGE or phage typing. The reproducibility of MLVA was evaluated by subtyping sequential isolates from an infected individual and by testing isolates following multiple passages and freeze-thaw cycles. PFGE and MLVA patterns were reproducible for isolates that were frozen and passaged multiple times. However, 2 of 12 sequential isolates obtained from an individual over the course of 36 days had an MLVA type that differed at one locus and one isolate had a different phage type. Overall, MLVA typing of Salmonella serotype Enteritidis had enhanced resolution, good reproducibility, and good epidemiological concordance. These results indicate that MLVA may be a useful tool for detection and investigation of outbreaks caused by Salmonella serotype Enteritidis.

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“…At present, there is not a consensus as to which method is best suited for differentiation of serotype Enteritidis strains. Comparison of antibiotic resistance patterns (19), plasmid profiles (32,38), IS200 restriction fragment length polymorphism (31), ribotyping (16)(17)(18), and pulsed-field gel electrophoresis (PFGE) (20,27,34,35) have been used to differentiate strains for epidemi-ological purposes with various degrees of success. Also several PCR-based methods, such as random amplification of polymorphic DNA, have been used for this purpose, but these methods may lack sensitivity and reproducibility for routine use, and they do not appear to be suitably discriminatory due to the inability to separate artefactual variation and true polymorphism (18,36).…”

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confidence: 99%

Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting

et al. 2001

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Reliable and sufficiently discriminative methods are needed for differentiating individual strains of Salmonella enterica serotype Enteritidis beyond the phenotypic level; however, a consensus has not been reached as to which molecular method is best suited for this purpose. In addition, data are lacking on the molecular fingerprinting of serotype Enteritidis from poultry environments in the United Kingdom. This study evaluated the combined use of classical methods (phage typing) with three well-established molecular methods (ribotyping, macrorestriction analysis of genomic DNA, and plasmid profiling) in the assessment of diversity within 104 isolates of serotype Enteritidis from eight unaffiliated poultry farms in England. The most sensitive technique for identifying polymorphism was PstI-SphI ribotyping, distinguishing a total of 22 patterns, 10 of which were found among phage type 4 isolates. Pulsed-field gel electrophoresis of XbaI-digested genomic DNA segregated the isolates into only six types with minor differences between them. In addition, 14 plasmid profiles were found among this population. When all of the typing methods were combined, 54 types of strains were differentiated, and most of the poultry farms presented a variety of strains, which suggests that serotype Enteritidis organisms representing different genomic groups are circulating in England. In conclusion, geographical and animal origins of Salmonella serotype Enteritidis isolates may have a considerable influence on selecting the best typing strategy for individual programs, and a single method cannot be relied on for discriminating between strains.

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The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases

Cited by 55 publications

References 31 publications

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni

Comparison of Multiple-Locus Variable-Number Tandem Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Phage Typing for Subtype Analysis of Salmonella enterica Serotype Enteritidis

Diversity of Strains of Salmonella enterica Serotype Enteritidis from English Poultry Farms Assessed by Multiple Genetic Fingerprinting

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