The Use of Phase Inversion Temperature (PIT) Microemulsion Technology to Enhance Oil Utilisation during Streptomyces rimosus Fed-batch Fermentations to Produce Oxytetracycline

Abstract

The use of a rapeseed oil emulsion feed, produced by a phase inversion temperature (PIT) process, produced more biomass, gave a 3-fold increase in oil utilisation and a higher oxytetracycline titre but a higher residual oil concentration when compared to a conventional fed-batch Streptomyces rimosus process fed with crude rapeseed oil. Importantly, microbial utilisation of the surfactant was confirmed for the first time.

Development of Fed-Batch Cultivation Strategy for Efficient Oxytetracycline Production by Streptomyces rimosus at Semi-Industrial Scale

“…Thus, fedbatch strategy was considered as alternative cultivation process by many researchers to increase antibiotic production by actinomycetes such as in case of rifamycins production by Amycolatopsis mediterranei (El Enshasy et al 2003), clavulanic acid by S. clavuligerus (Saudagar and Singhal 2007), and daptomycin by S. roseosporus (Ng et al 2014). Papapanagiotou et al (2005) and Hewitt et al (2007) investigated the application of emulsification technologies to enhance rapeseed oil consumption during OTC under fed-batch conditions. However, they aimed mainly to compare between the effect of feeding normal rapeseed oil and rapeseed oil emulsion produced by phase inversion temperature on the utilization of rapeseed oil.…”