The use of pAL5000 Replicon Vectors to Transform Rhodococcus and GordoniaSpecies

Abstract

pAL5000 based vectors, conventionally used to transform Mycobacterium species were used in this study to transform Rhodococcus and Gordonia species. Adequate transformation efficiencies were obtained ranging between 4.5x10 3 -2.8x10 6 . Additionally, a pAL5000 based vector with suicide function was generated. This vector was transformed into Rhodococcus, Mycobacterium and Gordonia species and was maintained as a stable construct.