An extensive and interdisciplinary literature review was carried out to evaluate the uses of synthetically produced ozone in many different application areas. The objective of the study was to investigate the disinfectant and purifying effectiveness of this natural compound and evaluate its use as an economically and environmentally sustainable alternative to treatments that often involve the adoption of pharmaceutical agents. Being a natural substance, the potential environmental sustainability of the use of ozone in areas such as water disinfectant; pesticide action in agriculture; and antibiotic, anti-inflammatory and antiviral actions in animal husbandry and fish farming are of interest. In addition to environmental sustainability, economic sustainability is also important for companies employing ozone in their processes. Thus, a case study was proposed that represents the use of ozone in a pilot swine farm located in Northern Italy, both as an alternative to pharmaceutical drugs for the animals and as an air and water purifier. The case study demonstrates the economic sustainability of ozone use, especially in the medium run, along with its ability to reduce animal mortality (by about 2%), as well as decrease use of pharmaceutical antibiotics.