The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance

Rusco, Giusy; Iorio, Michele Di; Esposito, Stefano; Antenucci, Emanuele; Roncarati, Alessandra; Iaffaldano, Nicolaia

doi:10.3390/vetsci10030219

Cited by 2 publications

(1 citation statement)

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“…Interestingly, natural sperm activation mode in medaka may be attributed to the interaction with the ovarian fluid. While fish sperm is typically activated in the surrounding water, the presence of ovarian fluid has been observed to enhance or extend the duration of sperm motility in zebrafish 18 and some other teleost 19 , 20 . In the present study, medaka sperm were activated at an osmolality of approximately 300 mOsm/kg, which corresponds to ovarian fluid osmolality 19 .…”

Section: Discussionmentioning

confidence: 99%

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sayyari,

Krogenæs,

Mayer

et al. 2024

Sci Rep

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Japanese medaka (Oryzias latipes) has been used as a model organism in different research fields, including reproductive physiology. Sperm motility is the most important marker for male fertility in fish and, thus, reproduction success. However, because of small volume of ejaculate and short motility duration, it is still challenging to manage the sperm collection and analysis in small model fish. In the present study, we aimed to investigate sperm motility and to optimize sperm collection, short-term sperm storage, and cryopreservation in Japanese medaka (Oryzias latipes). Using two different approaches for sperm collection: testes dissection and abdominal massage, different housing conditions and activating the sperm with different activation solutions, we investigated immediate sperm motility. In the second part of this study, we used different osmolalities of immobilization solution, Hank's Balanced Salt Solution (HBSS) for sperm storage at 0, 2 and 3 h after sperm collection. Finally, the sperm were cryopreserved using methanol as cryoprotectant and HBSS as extender at two different osmolalities, and post-thaw sperm motility was investigated. The highest post-activating sperm motility was achieved in the groups activated by the extender at 300 mOsm/kg. The quality of sperm remained unaffected by co-housing with females or with males only. Furthermore, Hanks’ Balanced Salt Solution (HBSS) with an osmolality of 600 mOsm/kg demonstrated its efficacy as a suitable extender for sperm storage, preserving motility and progressivity for 3 h. The highest post-thaw motility was around 35%. There were no significant differences between post-thaw motility in different groups. We also found that post-thaw incubation on ice can maintain the motility of the sperm for up to one hour after thawing.

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Section: Discussionmentioning

confidence: 99%

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sayyari,

Krogenæs,

Mayer

et al. 2024

Sci Rep

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Effects of Ammonia Concentration on Sperm Vitality, Motility Rates, and Morphology in Three Marine Bivalve Species: A Comparative Study of the Noble Scallop Mimachlamys nobilis, Chinese Pearl Oyster Pinctada fucata martensii, and Small Rock Oyster Saccostrea mordax

Li,

Wu,

Yang

et al. 2024

Biology

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Ammonium (NH4+) plays a crucial role in the reproductive processes of key biotic groups in aquatic ecosystems—bivalves. This study aims to elucidate the effects of three different ammonium ion concentrations on sperm vitality, swimming kinematics, and morphology of Mimachlamys nobilis, Pinctada fucata martensii, and Saccostrea mordax. The results indicate that the sperm vitality and motility rates of M.nobilis and S. mordax are inversely proportional to the ammonium concentration, especially in the treatment group with an ammonium concentration of 3 mmol/L, where the decrease in sperm vitality and motility is most significant. In contrast, the sperm of P. fucata martensii reacted differently to increasing ammonium concentrations. After the addition of 2 mmol/L of ammonium, the sperm vitality and motility of P. fucata martensii reached a peak, showing a significant stimulatory effect. Additionally, as the ammonium concentration increased, the curling of the sperm flagella in M.nobilis and S. mordax increased. However, sperm flagella curling in P. fucata martensii showed no change compared to the control group. This study provides insights into the effects of ammonium concentrations on the sperm vitality and motility of three marine bivalve species and highlights the importance of sperm flagella curling as a factor affecting sperm.

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The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance

Cited by 2 publications

References 50 publications

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Sperm handling and management in the teleost model fish Japanese medaka (Oryzias latipes)

Effects of Ammonia Concentration on Sperm Vitality, Motility Rates, and Morphology in Three Marine Bivalve Species: A Comparative Study of the Noble Scallop Mimachlamys nobilis, Chinese Pearl Oyster Pinctada fucata martensii, and Small Rock Oyster Saccostrea mordax

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