The use of organoids in food research

Stanton, Janelle E.; Grabrucker, Andreas M.

doi:10.1016/j.cofs.2022.100977

Cited by 4 publications

(2 citation statements)

References 86 publications

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“…( 1 ) which described the first stem cell-derived 3D intestinal organoid that differentiated into villus-crypt structures that encompassed key epithelial cell lineages found in vitro , various progressive models were developed over the past 10 years along with “intestine-on-a-chip” microfluidic bioengineered models for human and mice [reviewed in ( 2 )]. The development of livestock intestinal organoids is progressing although the application of livestock organoids to investigate pharmaceutical and neutraceutical components is lacking compared to the application of human organoids to investigate functional foods (reviewed in ( 3 )). Various chicken organoid models have been described, ranging from enterospheres or spheroids ( 4 ) to extracellular matrix (ECM) embedded organoids using mammalian culture methods.…”

Section: Introductionmentioning

confidence: 99%

Chicken intestinal organoids: a novel method to measure the mode of action of feed additives

Mitchell,

Sutton,

Elango

et al. 2024

Front. Immunol.

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There is a rapidly growing interest in how the avian intestine is affected by dietary components and feed additives. The paucity of physiologically relevant models has limited research in this field of poultry gut health and led to an over-reliance on the use of live birds for experiments. The development of complex 3D intestinal organoids or “mini-guts” has created ample opportunities for poultry research in this field. A major advantage of the floating chicken intestinal organoids is the combination of a complex cell system with an easily accessible apical-out orientation grown in a simple culture medium without an extracellular matrix. The objective was to investigate the impact of a commercial proprietary blend of organic acids and essential oils (OA+EO) on the innate immune responses and kinome of chicken intestinal organoids in a Salmonella challenge model. To mimic the in vivo prolonged exposure of the intestine to the product, the intestinal organoids were treated for 2 days with 0.5 or 0.25 mg/mL OA+EO and either uninfected or infected with Salmonella and bacterial load in the organoids was quantified at 3 hours post infection. The bacteria were also treated with OA+EO for 1 day prior to challenge of the organoids to mimic intestinal exposure. The treatment of the organoids with OA+EO resulted in a significant decrease in the bacterial load compared to untreated infected organoids. The expression of 88 innate immune genes was investigated using a high throughput qPCR array, measuring the expression of 88 innate immune genes. Salmonella invasion of the untreated intestinal organoids resulted in a significant increase in the expression of inflammatory cytokine and chemokines as well as genes involved in intracellular signaling. In contrast, when the organoids were treated with OA+EO and challenged with Salmonella, the inflammatory responses were significantly downregulated. The kinome array data suggested decreased phosphorylation elicited by the OA+EO with Salmonella in agreement with the gene expression data sets. This study demonstrates that the in vitro chicken intestinal organoids are a new tool to measure the effect of the feed additives in a bacterial challenge model by measuring innate immune and protein kinases responses.

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Section: Introductionmentioning

confidence: 99%

Chicken intestinal organoids: a novel method to measure the mode of action of feed additives

Mitchell,

Sutton,

Elango

et al. 2024

Front. Immunol.

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“…As the long-term culture of intestinal epithelial is generally not possible, up until a few years ago intestinal cell Iines were the only in vitro intestinal models available for testing the effects of molecules, including nutrients. Nevertheless, cultured cell lines, as a model, are very limited because of their immortalized nature and the lack of different cell types present in the intestinal epithelium [12]. Organoids, obtained from intestinal stem cells, contain all epithelial cell lines and overcome these limitations, being a much more physiological model.…”

Section: Introductionmentioning

confidence: 99%

Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children

Córdova,

Tena-Garitaonaindia,

Álvarez-Mercado

et al. 2024

IJMS

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Asthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function and immunity, and with the composition of the intestinal microbiota and food consumption. In this study, we aimed to establish an organoid model to test the hypothesis that the intestinal content of lean and obese, allergic, asthmatic children differentially regulates epithelial intestinal gene expression. A model of mouse jejunum intestinal organoids was used. A group of healthy, normal-weight children was used as a control. The intestinal content of asthmatic obese children differentially induced the expression of inflammatory and mitochondrial response genes (Tnf-tumor necrosis factor, Cd14, Muc13-mucin 13, Tff2-Trefoil factor 2 and Tff3, Cldn1-claudin 1 and 5, Reg3g-regenerating family member 3 gamma, mt-Nd1-NADH dehydrogenase 1 and 6, and mt-Cyb-mitochondrial cytochrome b) via the RAGE-advanced glycosylation end product-specific receptor, NF-κB-nuclear factor kappa b and AKT kinase signal transduction pathways. Fecal homogenates from asthmatic normal-weight and obese children induce a differential phenotype in intestinal organoids, in which the presence of obesity plays a major role.

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Editorial overview: Are we prepared for ground-breaking innovations in food science and technology?

Granato

2023

Current Opinion in Food Science

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The use of organoids in food research

Cited by 4 publications

References 86 publications

Chicken intestinal organoids: a novel method to measure the mode of action of feed additives

Chicken intestinal organoids: a novel method to measure the mode of action of feed additives

Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children

Editorial overview: Are we prepared for ground-breaking innovations in food science and technology?

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