The Use of NS1 Rapid Diagnostic Test and qRT-PCR to Complement IgM ELISA for Improved Dengue Diagnosis from Single Specimen

Teoh, Boon‐Teong; Sam, Sing‐Sin; Tan, Kian Lam; Johari, Jefree; Abd-Jamil, Juraina; Hooi, Poh Sim; AbuBakar, Sazaly

doi:10.1038/srep27663

Cited by 38 publications

(40 citation statements)

References 44 publications

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“…The serotype specific sensitivity and specificity showed that the RDT was less sensitive and specific in case of DENV‐3 in comparison to DENV‐1 and DENV‐2. Similar results are reported earlier …”

Section: Discussionsupporting

confidence: 93%

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Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT‐PCR

Shukla

Singh

Sharma

et al. 2017

Journal of Medical Virology

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The objective of this study was to demonstrate the utility of dengue virus (DENV) non structural protein 1 (NS1) based rapid diagnostic test (RDT) for use in tribal and difficult to reach areas for early dengue (DEN) diagnosis in acute phase patients and evaluate its sensitivity and specificity against DENV NS1 enzyme linked immune sorbent assay (ELISA) and real time reverse transcriptase polymerase chain reaction (qRT-PCR). The DENV NS1 RDT was used for preliminary diagnosis during outbreaks in difficult to reach rural and tribal areas. The diagnosis was confirmed by DENV NS1 ELISA in the laboratory. The samples were also tested and serotyped by qRT-PCR. The results were evaluated using statistical tests. The DENV NS1 RDT showed 99.2% sensitivity and 96.0% specificity when analyzed using DENV NS1 ELISA as standard. The specificity and sensitivity of the RDT when compared with qRT-PCR was 93.6% and 91.1%, respectively. The serotype specific evaluation showed more than 90% sensitivity and specificity for DENV-1, 2, and 3. The RDT proved a good diagnostic tool in difficult to reach rural and tribal areas. Further evaluation studies with different commercially available RDTs in different field conditions are essential, that will help clinicians and patients for treatment and programme managers for timely intervention.

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Section: Discussionsupporting

confidence: 93%

“…These tests are economical and are easy to perform. Several studies have reported evaluation of RDTs, using different study designs and comparing the RDTs with ELISA, RT‐PCR, and qRT‐PCR in different conditions . But there are limited studies documenting DENV serotype specific evaluation of RDTs.…”

Section: Introductionmentioning

confidence: 99%

Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT‐PCR

Shukla

Singh

Sharma

et al. 2017

Journal of Medical Virology

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“…Detecting viral Non Structural glycoprotein 1 (NS1) in the blood of patients represents an alternative path in the early diagnosis of flaviviruses. The assay can also be useful for epidemiological surveillance …”

Section: Introductionmentioning

confidence: 99%

“…The assay can also be useful for epidemiological surveillance. [5][6][7] NS1, a secreted viral glycoprotein produced by infected cells, is synthesized as a monomer and is dimerized rapidly after post-translational modifications in the endoplasmic reticulum. Dimers are transported to the cell surface and associate as hexamers that accumulate in large amounts in the extracellular medium.…”

Section: Introductionmentioning

confidence: 99%

Paper‐based point‐of‐care testing for cost‐effective diagnosis of acute flavivirus infections

Bedin¹,

Boulet²,

Voilin³

et al. 2017

Journal of Medical Virology

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Flavivirus infections are a serious healthcare concern in tropical and subtropical countries. Although well-established laboratory tests can provide early diagnosis of acute dengue or Zika infections, access to these tests is limited in developing countries, presenting an urgent need to develop simple, rapid, and robust diagnostic tools. Microfluidic Paper-based Analytical Devices (μPAD), are typically rapid, cost-effective, user-friendly, and they can be used as diagnostic tools for the diagnosis of these infections at Point of Care settings. Early and prompt diagnosis is crucial to improve patient management and reduce the risk of complications. In the present study, we developed and evaluated a wax-printed paper-based device for the detection of the dengue and Zika non-structural NS1 viral protein in blood and plasma. Experiments have been carried out to increase specificity, while maintaining the required sensitivity. As a consequence, the quality of the raw materials and the washing steps were proved to be crucial. The μPAD was able to detect specifically in 6-8 min 10 ng/mL of protein in various sample types. A prototype for the differential detection of dengue and/or Zika NS1 protein was developed. The reading of the results was simplified by using a dedicated application on a smartphone.

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“…In secondary DENV infection, however, a decreased sensitivity of the NS-1 Ag Strip test was observed. 43 The roles of DENV NS-1 antigen and lipid mediators such as (Platelet-Activating Factor) PAF in causing vascular leak are emerging DENV NS-1 are likely to be helpful in reducing disease pathogenesis due to NS-1, drugs that block PAF receptors or the pathways in which PAF is generated may be helpful in the treatment of acute illness. 20 PAF was previously found to be an important contributor to vascular leak and PAF receptor blockade was found to inhibit the effects of acute dengue sera on the expression of the tight junction protein ZO-1, and in the reduction of trans-endothelial resistance.…”

Section: Discussionmentioning

confidence: 99%

MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN

Agustiningrum

Nugraha

Kahar

2020

IJTID

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Dengue infection caused by DENV and transmitted by mosquitoes Aedes aegypti and Aedes albopictus is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection are very widely, from asymptomatic until dengue shock syndrome (DSS). DENV will attack macrophages and dendritic cells (DC) and replicate them. Monocytes are macrophages in the blood (± 10% leukocytes). Macrophages produce cytokines and chemokines such as monocyte chemotactic protein-1 (MCP-1)/CCL2. The monocytes that are infected with DENV will express MCP-1, which will increase the permeability of vascular endothelial cells so that they have a risk of developing DHF/DSS. Macrophages and DC secrete NS1 proteins, which are the co-factors that are needed for viral replication and can be detected in the early phase of fever. The increased MCP-1 levels in dengue infection followed by an increase in the number of atypical lymphocytes indicate the arrival of macrophages and monocytes to the site of inflammation which triggers proliferation rather than lymphocytes. This is an observational analytical study with a cross-sectional design to determine the MCP-1 level in dengue infection patients with 1st until the 4th day of fever and the presence of a typical lymphocytes. Dengue infection was determined by rapid tests NS1 positive or negative and MCP-1 levels were measured using by ELISA sandwich method.MCP-1 level of sixty patients dengue infection NS-1 rapid positive or negative with 2nd until 4rt fever were significantly higher than healthy subjects (420.263 ± 158,496 vs 29, 475 ± 23.443;p=0.000), but there was no significant difference in subjects with DF, DHF or DSS (436,47 ± 225,59 vs 422,77 ± 170,55 vs 448,50 ± 117,39; p =0.844). A typically lymphocytes differs significantly in healthy subjects than subjects infected with DENV an average of 2% (p= 0,000). In conclusion, this shows the arrival of macrophages and monocytes to the site of inflammation, which triggers the proliferation of lymphocytes. ABSTRAK Infeksi dengue disebabkan DENV ditularkan oleh nyamuk Aedes aegypti dan Aedes albopictus yang masalah kesehatan utama di dunia, termasuk Indonesia. Manifestasi klinik infeksi dengue sangat bervariasi, dapat asimptomatik sampai dengue shock syndrome (DSS). DENV akan menyerang makrofag juga sel dendritik (DC) dan akan bereplikasi. Monosit merupakan makrofag dalam darah (±10% leukosit). Makrofag memproduksi sitokin dan kemokin seperti MCP 1. MCP-1 terekspresi oleh monosit terinfeksi DENV yang dapat meningkatkan permeabilitas sel endotel vaskular sehingga memiliki risiko mengalami DHF/ DSS. Makrofag dan DC mengeluarkanCorresponding Author.protein NS1 merupakan co-factor yang dibutuhkan untuk replikasi virus dan dapat dideteksi pada fase awal demam.Adanya peningkatan kadar MCP-1 pada infeksi dengue diikuti dengan peningkatan jumlah limfosit biru menunjukkan datangnya makrofag dan monosit ke tempat terjadinya inflamasi yang memicu proliferasi daripada limfosit. Penelitian ini merupakan penelitian observasional analitik ...

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The Use of NS1 Rapid Diagnostic Test and qRT-PCR to Complement IgM ELISA for Improved Dengue Diagnosis from Single Specimen

Cited by 38 publications

References 44 publications

Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT‐PCR

Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT‐PCR

Paper‐based point‐of‐care testing for cost‐effective diagnosis of acute flavivirus infections

MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN

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