The Use of Nicotiana Species in Tobacco Improvement

Berbeć, A.; Doroszewska, Teresa

doi:10.1007/978-3-030-29493-9_8

Cited by 9 publications

(5 citation statements)

References 174 publications

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“…Interspecific hybrids (amphihaploids) of N. glauca Grah. and N. langsdorfii Weinm., having a haploid set of chromosomes from each of the parents, regularly showed a relatively high percentage of unreduced gametes [14]. Amphihaploids of N. tabacum × N. glauca shows a failure of one of the meiotic divisions, leading to the formation of restitution nuclei and consequently the production of 2n male gametes [32].…”

Section: Naturally Occurring Unreduced Gametesmentioning

confidence: 99%

“…The simplest formula describing this group of polyploids is the notation AABB, where the A genome is derived from one and the B genome from the other parental species. Economically important allopolyploids include Gossypium hirsutum L., Miscanthus × giganteus Greef et Deu., Nicotiana tabacum L., Saccharum officinarum L. and Arachis hypogaea L. [12][13][14][15][16]. The next described type are segmental allopolyploids, i.e., forms intermediate between allopolyploids and autopolyploids.…”

Section: Introductionmentioning

confidence: 99%

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Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding

et al. 2021

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Polyploidisation is an important process in the evolution of many plant species. An additional set of chromosomes can be derived from intraspecific genome duplication (autopolyploidy) or hybridising divergent genomes and chromosome doubling (allopolyploidy). Special forms of polyploidy are autoallopolyploidy and segmental allopolyploidy. Polyploidy arises from two basic processes: spontaneously occurring disturbances of meiotic division and induced by antimitotic agents’ disruption of mitosis. The first involves the induction and fusion of unreduced gametes, resulting in the formation of triploids and tetraploids. The second process uses antimitotics that disrupt cellular microtubules and prevent chromosome’s sister chromatids motion during anaphase. Colchicine, oryzalin, and trifluralin are the most commonly used antimitotics for inducing polyploids in plants. The exposure time and concentration of the antimitotics and the species, cultivar, genotype, and tissue type affect the efficiency of genome duplication. Polyploids are distinguished from diploids by increased cell size and vegetative parts of plants and increased content of secondary metabolites. Genome duplication generates several changes at the epigenetic level resulting in altered gene expression. Polyploidisation is used in plant breeding to overcome the non-viability and infertility of interspecific hybrids, obtain seedless polyploid cultivars and increase resistance/tolerance to biotic and abiotic factors.

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Section: Naturally Occurring Unreduced Gametesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding

et al. 2021

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“…To survive these challenges, it has evolved a suite of adaptive systems that confer protection against specific threats. Several resistance loci have been identified in N. alata , including but not limited to, resistance to Tomato spotted wilt virus ( RTSW ), Tobacco mosaic virus ( N’ala ) and black root rot ( Yuan et al., 2015 ; Trojak-Goluch et al., 2016 ; Huang et al., 2018 ; Berbeć and Doroszewska, 2020 ; Czubacka, 2022 ; Li et al., 2023 ). More important, N. alata is a model species used to study gametophytic self-incompatibility (SI), controlled by the S -locus ( Bredemeijer and Blaas, 1981 ).…”

Section: Introductionmentioning

confidence: 99%

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco (Nicotiana alata) genome editing

Yuan,

Zeng,

Liu

et al. 2024

Front. Plant Sci.

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Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. Nicotiana alata is a typical wild species of genus Nicotiana that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits. However, it lacks an efficient genetic transformation and genome editing system, which hampers its gene function and breeding research. In this study, we developed an optimized hypocotyl-mediated transformation method for CRISPR-Cas9 delivery. The genetic transformation efficiency was significantly improved from approximately 1% to over 80%. We also applied the CRISPR-Cas9 system to target the phytoene desaturase (NalaPDS) gene in N. alata and obtained edited plants with PDS mutations with over 50% editing efficiency. To generate self-compatible N. alata lines, a polycistronic tRNA-gRNA (PTG) strategy was used to target exonic regions of allelic S-RNase genes and generate targeted knockouts simultaneously. We demonstrated that our system is feasible, stable, and high-efficiency for N. alata genome editing. Our study provides a powerful tool for basic research and genetic improvement of N. alata and an example for other wild tobacco species.

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“…For this purpose, wild species are useful as genetic resources, and several resistance genes, such as tobacco mosaic virus resistance gene N from N. glutinosa [92][93][94][95][96], and black shank resistance genes Php from N. plumbaginifolia and Phl from N. longiflora [97,98], have been introduced into N. tabacum by interspecific crossings. Another important usefulness of wild species is as a source of cytoplasmic male sterility in N. tabacum [99][100][101][102][103][104][105][106][107][108].…”

Section: Introductionmentioning

confidence: 99%

Genetic Cause of Hybrid Lethality Observed in Reciprocal Interspecific Crosses between Nicotiana simulans and N. tabacum

Tezuka,

Nagai,

Matsuo

et al. 2024

IJMS

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Hybrid lethality, a type of postzygotic reproductive isolation, is an obstacle to wide hybridization breeding. Here, we report the hybrid lethality that was observed in crosses between the cultivated tobacco, Nicotiana tabacum (section Nicotiana), and the wild tobacco species, Nicotiana simulans (section Suaveolentes). Reciprocal hybrid seedlings were inviable at 28 °C, and the lethality was characterized by browning of the hypocotyl and roots, suggesting that hybrid lethality is due to the interaction of nuclear genomes derived from each parental species, and not to a cytoplasmic effect. Hybrid lethality was temperature-sensitive and suppressed at 36 °C. However, when hybrid seedlings cultured at 36 °C were transferred to 28 °C, all of them showed hybrid lethality. After crossing between an N. tabacum monosomic line missing one copy of the Q chromosome and N. simulans, hybrid seedlings with or without the Q chromosome were inviable and viable, respectively. These results indicated that gene(s) on the Q chromosome are responsible for hybrid lethality and also suggested that N. simulans has the same allele at the Hybrid Lethality A1 (HLA1) locus responsible for hybrid lethality as other species in the section Suaveolentes. Haplotype analysis around the HLA1 locus suggested that there are at least six and two haplotypes containing Hla1-1 and hla1-2 alleles, respectively, in the section Suaveolentes.

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The Use of Nicotiana Species in Tobacco Improvement

Cited by 9 publications

References 174 publications

Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding

Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding

Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco (Nicotiana alata) genome editing

Genetic Cause of Hybrid Lethality Observed in Reciprocal Interspecific Crosses between Nicotiana simulans and N. tabacum

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