The use of new hydrogel microcapsules in coconut juice as biocatalyst system for the reaction of quinine

Fonseca, Aluísio Marques da; Santos, José Cleiton Sousa dos; Souza, Monique; Oliveira, Mauro Macêdo de; Colares, Regilany Paulo; Lemos, Telma L. G.; Braz-Filho, Raimundo

doi:10.1016/j.indcrop.2019.111890

Cited by 20 publications

(7 citation statements)

References 19 publications

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“…Enzymatic immobilization in MOFs is achieved by the formation of bonds between the enzyme and their interactions with the material [7,69,70]. Most synthesized enzyme-MOF compounds are based on the mechanisms of co-inclusion, covalent bonding, trapping, or physical adsorption.…”

Section: Preparation/characterization Of Enzyme-mof Biosensorsmentioning

confidence: 99%

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

et al. 2022

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Several studies have shown the development of electrochemical biosensors based on enzymes immobilized in metal–organic frameworks (MOFs). Although enzymes have unique properties, such as efficiency, selectivity, and environmental sustainability, when immobilized, these properties are improved, presenting significant potential for several biotechnological applications. Using MOFs as matrices for enzyme immobilization has been considered a promising strategy due to their many advantages compared to other supporting materials, such as larger surface areas, higher porosity rates, and better stability. Biosensors are analytical tools that use a bioactive element and a transducer for the detection/quantification of biochemical substances in the most varied applications and areas, in particular, food, agriculture, pharmaceutical, and medical. This review will present novel insights on the construction of biosensors with materials based on MOFs. Herein, we have been highlighted the use of MOF for biosensing for biomedical, food safety, and environmental monitoring areas. Additionally, different methods by which immobilizations are performed in MOFs and their main advantages and disadvantages are presented.

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Section: Preparation/characterization Of Enzyme-mof Biosensorsmentioning

confidence: 99%

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

et al. 2022

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“…Furthermore, coaggregation agents (co-feeders) such as polymers or proteins are suggested to enhance inter-enzyme crosslinking [115]. It has been successfully applied in the immobilization of several different types of enzymes such as hydrolases [90], oxidoreductases [116], lyases [117], transferases [118], and isomerases [119] used in the manufacturing of chemical products, being subject to an increasing number of analyses [120,121].…”

Section: Crosslinked Enzyme Aggregates (Cleas)mentioning

confidence: 99%

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Cavalcante

Sousa

et al. 2021

Catalysts

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The market for industrial enzymes has witnessed constant growth, which is currently around 7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes naturally responsible for triglyceride hydrolysis. They are the most expansively used industrial biocatalysts, with wide application in a broad range of industries. However, these biocatalytic processes are usually limited by the low stability of the enzyme, the half-life time, and the processes required to solve these problems are complex and lack application feasibility at the industrial scale. Emerging technologies create new materials for enzyme carriers and sophisticate the well-known immobilization principles to produce more robust, eco-friendlier, and cheaper biocatalysts. Therefore, this review discusses the trending studies and industrial applications of the materials and protocols for lipase immobilization, analyzing their advantages and disadvantages. Finally, it summarizes the current challenges and potential alternatives for lipases at the industrial level.

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“…Therefore, to ensure lipases having desired activity in organic systems, it is required that there is an environment like the water/oil interface in pure organic phase. For example, the alginate, in which enzyme proteins was encapsulated, was ever reported to contribute to the maintenance of enzyme activity [55]. Moreover, the cashew apple bagasse could make the lipase covalently immobilized on it having desired stability in resolution of rac-indanyl acetate [15].…”

Section: Design and Immobilization Of Pfl On Rice Strawmentioning

confidence: 99%

Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate

Yuan

Cong

Zhai

et al. 2021

Bioprocess Biosyst Eng

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Citronellyl acetate as an important flavor, can be effectively synthesized by lipase catalysis in nonaqueous system. But lipases usually behave low catalytic activity due to aggregation and denaturation of them in organic phase. To enhance the nonaqueous catalysis, based on the mechanism of lipases activated at water/oil (organic phase) interface, the inexpensive race straw was processed into powder and filaments on which Pseudomonas fluorescens lipase was immobilized by physical adsorption, used for synthesis of citronellyl acetate via transesterification of citronellol and vinyl acetate. Results showed that the desired loading was 10 mg lipase immobilized on 30 mg rice straw filaments or 25 mg rice straw powder. When the two immobilized lipases were employed in the reaction system consisted of 1-mL citronellol and 2-mL vinyl acetate at 37 ℃ and 160 rpm, the conversions all reached 99.8% after 12 h. Under the reaction condition, the conversion catalyzed by 10 mg native lipase was 85.1%. Undergoing six times of 8-h reuses in the organic system, the filament and power immobilized lipases had weak activity attenuation rates 0.36 and 0.32% h −1 , lower than 1.52% h −1 of native lipase. Even at the room temperature and the static state without shaking and stirring, the rice straw filaments immobilized lipase could brought conversion 62.9% after 10 h but the native lipase only gave 37.0%. Obviously, the rice straw, especially its filaments, is an inexpensive and available natural material to prepare immobilized lipase with desired catalysis in organic phase, meant significant potential in flavor industry.

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The use of new hydrogel microcapsules in coconut juice as biocatalyst system for the reaction of quinine

Cited by 20 publications

References 19 publications

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

A Comprehensive Review on the Use of Metal–Organic Frameworks (MOFs) Coupled with Enzymes as Biosensors

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate

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