The use of Nasal Epithelial Stem/progenitor Cells to Produce Functioning Ciliated Cells <i>in vitro</i>

Zhao, Xuening; Yu, F.; Li, Chunwei; Li, Yingying; Chao, Siew-Shuen; Loh, Woei-Shyang; Pan, Xinliang; Li, Shi; Wang, Deyun

doi:10.2500/ajra.2012.26.3794

Cited by 45 publications

(42 citation statements)

References 21 publications

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“…Finally, future studies should investigate the proliferation, maintainenance, and differentiation of ΔNp63 + basal cells, which were identified in our study, and have been recently described as adult tissue stem cells of nasal epithelium [45]. The biology and relevance of stem cells in nasal epithelium remain unclear.…”

Section: Discussionmentioning

confidence: 79%

“…The antibody used (clone 4A1, Santa Cruz) detects the ΔN isoform of p63 (ΔNp63), which has been identified as basal stem/progenitor cell marker in human [27], [40]–[42] and rodent [40], [41], [43], [44] tracheobronchial epithelium, as well as in human nasal epithelium [21], [45]. It has been reported that basal stem/progenitor cells are implicated in the regeneration of human tracheobronchial [42], [46] and nasal [21], [45] epithelium. Therefore, the reconstitution of both NP and control NM epithelia in our ALI cultures may be attributed, at least in part, to the identified ΔNp63 + basal stem/progenitor population.…”

Section: Discussionmentioning

confidence: 99%

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Reconstituted Human Upper Airway Epithelium as 3-D In Vitro Model for Nasal Polyposis

et al. 2014

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BackgroundPrimary human airway epithelial cells cultured in an air-liquid interface (ALI) develop a well-differentiated epithelium. However, neither characterization of mucociliar differentiation overtime nor the inflammatory function of reconstituted nasal polyp (NP) epithelia have been described.Objectives1st) To develop and characterize the mucociliar differentiation overtime of human epithelial cells of chronic rhinosinusitis with nasal polyps (CRSwNP) in ALI culture system; 2nd) To corroborate that 3D in vitro model of NP reconstituted epithelium maintains, compared to control nasal mucosa (NM), an inflammatory function.MethodsEpithelial cells were obtained from 9 NP and 7 control NM, and differentiated in ALI culture for 28 days. Mucociliary differentiation was characterized at different times (0, 7, 14, 21, and 28 days) using ultrastructure analysis by electron microscopy; ΔNp63 (basal stem/progenitor cell), β-tubulin IV (cilia), and MUC5AC (goblet cell) expression by immunocytochemistry; and mucous (MUC5AC, MUC5B) and serous (Lactoferrin) secretion by ELISA. Inflammatory function of ALI cultures (at days 0, 14, and 28) through cytokine (IL-8, IL-1β, IL-6, IL-10, TNF-α, and IL-12p70) and chemokine (RANTES, MIG, MCP-1, IP-10, eotaxin-1, and GM-CSF) production was analysed by CBA (Cytometric Bead Array).ResultsIn both NP and control NM ALI cultures, pseudostratified epithelium with ciliated, mucus-secreting, and basal cells were observed by electron microscopy at days 14 and 28. Displaying epithelial cell re-differentation, β-tubulin IV and MUC5AC positive cells increased, while ΔNp63 positive cells decreased overtime. No significant differences were found overtime in MUC5AC, MUC5B, and lactoferrin secretions between both ALI cultures. IL-8 and GM-CSF were significantly increased in NP compared to control NM regenerated epithelia.ConclusionReconstituted epithelia from human NP epithelial cells cultured in ALI system provides a 3D in vitro model that could be useful both for studying the role of epithelium in CRSwNP while developing new therapeutic strategies, including cell therapy, for CRSwNP.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Reconstituted Human Upper Airway Epithelium as 3-D In Vitro Model for Nasal Polyposis

et al. 2014

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“…Similarly, Hegab et al (2012) have demonstrated that NGFR + CD166 + CD44 + epithelial cells, isolated from human trachea and submucosal glands, can generate luminal colonies comprising K5 + K14 + basal cells and mucous and serous secretory epithelial cells. Zhao et al (2012) showed that primary p63 + K5 + basal cells taken from the inferior turbinate in the nose also exhibited proliferative potential and differentiated into ciliated and goblet cells in ALI cultures. A recent study has also shown that basal cells exhibit heterogeneous profiles in different disease contexts.…”

Section: Human Lung Epithelial Stem and Progenitor Cellsmentioning

confidence: 97%

Harnessing the potential of lung stem cells for regenerative medicine

McQualter

Anthony

Bozinovski

et al. 2014

The International Journal of Biochemistry & Cell Biology

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“…In addition, some studies have demonstrated the existence of human nasal epithelial stem/progenitor cells. These cells can be isolated and propagated in vitro and exhibit some stem-cell properties during serial passage [66,67].…”

Section: Nasal Epithelial Cellsmentioning

confidence: 99%

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

Zhang

2015

Regen. Med.

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Trachea-tissue engineering is a thriving new field in regenerative medicine that is reaching maturity and yielding numerous promising results. In view of the crucial role that the epithelium plays in the trachea, re-epithelialization of tracheal substitutes has gradually emerged as the focus of studies in tissue-engineered trachea. Recent progress in our understanding of stem cell biology, growth factor interactions and transplantation immunobiology offer the prospect of optimization of a tissue-engineered tracheal epithelium. In addition, advances in cell culture technology and successful applications of clinical transplantation are opening up new avenues for the construction of a tissue-engineered tracheal epithelium. Therefore, this review summarizes current advances, unresolved obstacles and future directions in the reconstruction of a tissue-engineered tracheal epithelium.

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The use of Nasal Epithelial Stem/progenitor Cells to Produce Functioning Ciliated Cells in vitro

Cited by 45 publications

References 21 publications

Reconstituted Human Upper Airway Epithelium as 3-D In Vitro Model for Nasal Polyposis

Reconstituted Human Upper Airway Epithelium as 3-D In Vitro Model for Nasal Polyposis

Harnessing the potential of lung stem cells for regenerative medicine

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

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