The Use of N-Butyl Alcohol in Dehydrating Woody Tissue for Paraffin Embedding

The Occurrence and Types of Vessels in the Various Organs of the Plant in the Monocotyledoneae

“…Indeed, the only paper of any real importance in this respect is one by Caspary published in 1862. In their review of the comparative anatomy of the Monocotyledoneae, however, Solereder and Meyer (1928, 1929, 1930, 1933 draw a clear distinction between tracheids and vessels, but the information in this important series of publications is fragmentary. Numerous publications have appeared in which the general topography of the vascular tissues in many groups of plants has been discussed, but in which only incidental observations concerning the true nature of the tracheary elements have been recorded.…”

The Floral Morphology of Some of the Capparidaceae

“…Fresh buds and flowers were fixed in 70 per cent ethyl alcohol, dehydrated according to Zirkle's (1930) butyl-alcohol method and imbedded in paraffin. Sections 10 or 15 microns thick were mounted serially and stained with safranin and either Delafield's haematoxylin or fast green.…”

Mitochondria in the Life Cycles of Certain Higher Plants

“…By far the most satisfactory fluid, however, was Zirkle's (1934) modification of Erliki's potassium bichromate-copper sulphate mixture in the following proportions: K2Cr207, 1.25 g.; (NH4)2Cr207, 1.25 g.; CuSo4, 1.00 g.; H20, 200.00 g. Enough pyridine was added to this mixture to make 1/4 of 1 per cent. After the resulting precipitate had settled (which required about 20 minutes), specimens were placed in the fluid for 48 hours.…”

“…After the resulting precipitate had settled (which required about 20 minutes), specimens were placed in the fluid for 48 hours. They were then washed in water for an hour, dehydrated in butyl alcohol (Zirkle, 1930) and embedded in paraffin. Sections were generally cut 8 microns in thickness.…”