The use of monolithic polymeric sorbents to simulate virus-cell interactions

A noncompetitive peak decay method was used with 1 mm × 4.6 mm i.d. silica monoliths to measure the dissociation rate constants (kd) for various drugs with human serum albumin (HSA) and α1-acid glycoprotein (AGP). Flow rates up to 9 mL/min were used in these experiments, resulting in analysis times of only 20-30 s. Using a silica monolith containing immobilized HSA, dissociation rate constants were measured for amitriptyline, carboplatin, cisplatin, chloramphenicol, nortriptyline, quinidine, and verapamil, giving values that ranged from 0.37 s−1 to 0.78 s−1. Similar work with an immobilized AGP silica monolith gave kd values for amitriptyline, nortriptyline, and lidocaine of 0.39 s−1 to 0.73 s−1. These kd values showed good agreement with values determined for drugs with similar structures and/or affinities for HSA or AGP. It was found that a kd of up to roughly 0.80 s−1 could be measured by this approach. This information made it possible to obtain a better understanding of the advantages and possible limitations of the noncompetitive peak decay method and in the use of affinity silica monoliths for the high-throughput analysis of drug-protein dissociation.

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High‐throughput analysis of drug dissociation from serum proteins using affinity silica monoliths

Yoo

Hage

2011

“…Various support materials have been considered for use in AMC, including GMA/EDMA co-polymers [7–17], agarose [18,19] and cryogels [20,21], among others. Silica monoliths are another set of supports that have been explored for use in AMC [22–26].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of silica monoliths in affinity microcolumns for high‐throughput analysis of drug–protein interactions

Yoo

Hage²

2009

Silica monoliths in affinity microcolumns were tested for the high-throughput analysis of drugprotein interactions. Human serum albumin (HSA) was used as a model protein for this work, while carbamazepine and R-warfarin were used as model analytes. A comparison of HSA silica monoliths of various lengths indicated columns as short as 1 to 3 mm could be used to provide reproducible estimates of retention factors or plate heights. Benefits of using smaller columns for this work included the lower retention times and lower back pressures that could be obtained versus traditional HPLC affinity columns, as well as the smaller amount of protein that is required for column preparation. One disadvantage of decreasing column length was the lower precision that resulted in retention factor and plate height measurements. A comparison was also made between microcolumns containing silica particles versus silica monoliths. It was demonstrated with R-warfarin that supports could be used in HSA microcolumns for the determination of retention factors or plate heights. However, the higher efficiency of the silica monolith made this the preferred support for work at higher flow rates or when a larger number of plates are needed during the rapid analysis of drug-protein interactions.

“…Last but not least, complexes between enzymes and their inhibitors are one of the major systems targeted by AC. Monolithic supports with immobilized soybean trypsin inhibitor were used to capture virus‐like particles that had been modified with trypsin 149, 150. Another report described the purification of penicillin acylase from crude extracts of Penicillium cultures by immobilized antipyrine 151.…”

Section: Types Of Acmentioning

confidence: 99%

Monolithic media for applications in affinity chromatography

Sproß

Sinz

2011

Affinity chromatography presents a highly versatile analytical tool, which relies on exploiting highly specific interactions between molecules and their ligands. This review covers the most recent literature on the application of monoliths as stationary phases for various affinity-based chromatographic applications. Different affinity approaches as well as separations using molecularly imprinted monoliths are discussed. Hybrid stationary phases created by embedding of particles or nanoparticles into a monolithic stationary phase are also considered in this review article. The ease of preparation of monoliths and the multitude of functionalization techniques, which have matured during the past years, make monoliths interesting for an increasing number of biochemical and medical applications.