The Use of Molecularly Imprinted Polymers for Sampling and Sample Preparation

Sellergren, Börje; Martín‐Esteban, Antonio

doi:10.1002/9780813823621.ch23

Cited by 10 publications

(7 citation statements)

References 218 publications

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“…From the last few decades, MIP-based solid-phase extraction (SPE) is the most commonly used efficient sample preparation method introduced by Sellergren for the first time in the analytical sciences . The MIP-SPE technique can selectively extract the desired analytes and separate interfering compounds from complex samples; typically, extraction is performed in organic-rich aqueous solvent workflows . The key-limiting factor for the imprinted receptor prepared with the conventional imprinting protocol is water incompatibility, resulting in poor recognition of imprinted analytes in 100% aqueous media .…”

Section: Introductionmentioning

confidence: 99%

“…21 The MIP-SPE technique can selectively extract the desired analytes and separate interfering compounds from complex samples; typically, extraction is performed in organic-rich aqueous solvent workflows. 22 The key-limiting factor for the imprinted receptor prepared with the conventional imprinting protocol is water incompatibility, resulting in poor recognition of imprinted analytes in 100% aqueous media. 23 In bioanalysis where recognition media are either blood, plasma, urine, saliva, or aqueous buffers, imprinted polymers have seen limited success as compared to biological receptors.…”

Section: ■ Introductionmentioning

confidence: 99%

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Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

Rouhi

İncel

Shinde

2021

ACS Appl. Polym. Mater.

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The field of anion recognition in aqueous media is dominated by multiple positive charges and/or metal ions containing synthetic receptors. Here, we report the synthesis of a highly water-compatible oxyanion-imprinted neutral receptor by cross-linking the polymerizable diaryl urea host monomer and phenyl phosphonic acid (PPA) complex in the presence of a large number of comonomers containing hydroxyl or methyl ester groups. We have demonstrated that the affinity of the imprinted receptor for anions is easily tuned by decorating the hydrophilic and hydrophobic end groups around the binding pocket of the imprinted receptor. The binding characteristics of the synthesized artificial binders were evaluated in aqueous and nonaqueous environments. We have demonstrated practical applicability of the PPA-imprinted receptor by packing in the solid-phase extraction cartridge which was used to separate the ubiquitous pesticide glyphosate from water. Also, the developed receptor can selectively bind to phosphorylated tyrosine amino acids and phosphorylated tyrosine octapeptides in aqueous buffered media than to other phosphorylated and nonphosphorylated analogues. The approach utilized here to use an inexpensive dummy template can be utilized for the synthesis of imprinted receptors for anions with phosphate motifs.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

Rouhi

İncel

Shinde

2021

ACS Appl. Polym. Mater.

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“…One of the most useful applications of MIPs is their use as a sorbent in SPE [17,18]. Here, the sample is passed through a cartridge (or a packed column) filled with a solid sorbent to which the analytes bind and are released in the presence of specific solvents (Figure S1).…”

Section: Introductionmentioning

confidence: 99%

Tailored Polymer-Based Selective Extraction of Lipid Mediators from Biological Samples

et al. 2021

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Lipid mediators, small molecules involved in regulating inflammation and its resolution, are a class of lipids of wide interest as their levels in blood and tissues may be used to monitor health and disease states or the effect of new treatments. These molecules are present at low levels in biological samples, and an enrichment step is often needed for their detection. We describe a rapid and selective method that uses new low-cost molecularly imprinted (MIP) and non-imprinted (NIP) polymeric sorbents for the extraction of lipid mediators from plasma and tissue samples. The extraction process was carried out in solid-phase extraction (SPE) cartridges, manually packed with the sorbents. After extraction, lipid mediators were quantified by liquid chromatography–tandem mass spectrometry (LC–MSMS). Various parameters affecting the extraction efficiency were evaluated to achieve optimal recovery and to reduce non-specific interactions. Preliminary tests showed that MIPs, designed using the prostaglandin biosynthetic precursor arachidonic acid, could effectively enrich prostaglandins and structurally related molecules. However, for other lipid mediators, MIP and NIP displayed comparable recoveries. Under optimized conditions, the recoveries of synthetic standards ranged from 62% to 100%. This new extraction method was applied to the determination of the lipid mediators concentration in human plasma and mouse tissues and compared to other methods based on commercially available cartridges. In general, the methods showed comparable performances. In terms of structural specificity, our newly synthesized materials accomplished better retention of prostaglandins (PGs), hydroxydocosahexaenoic acid (HDoHE), HEPE, hydroxyeicosatetraenoic acids (HETE), hydroxyeicosatrienoic acid (HETrE), and PUFA compounds, while the commercially available Strata-X showed a higher recovery for dihydroxyeicosatetraenoic acid (diHETrEs). In summary, our results suggest that this new material can be successfully implemented for the extraction of lipid mediators from biological samples.

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“…Molecularly imprinted polymers (MIPs) have been employed over the last years (Whitcombe et al, 2014) as a useful tool for different analytical applications including chromatography (Sulitzky et al, 2002;Chen et al, 2012), capillary electrochromatography (Turiel and Martin-Esteban, 2005), sensor technology (Piletsky et al, 2012) or even in sample treatment procedures such as solid-phase extraction (Sellergren and Esteban, 2010), solid phase microextraction (Prasad et al, 2008) or stir bar sorptive extraction (Gomez-Caballero et al, 2011). In what concerns chromatography, MIP-based stationary phases, traditionally, have been almost exclusively synthesized by bulk polymerization, obtaining irregular particles because of grinding and sieving processes after radical polymerization.…”

Section: Introductionmentioning

confidence: 99%

Iniferter‐mediated grafting of molecularly imprinted polymers on porous silica beads for the enantiomeric resolution of drugs

Gutiérrez-Climente

Gómez‐Caballero

Halhalli

et al. 2015

J of Molecular Recognition

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A surface-imprinted chiral stationary phase for the enantiomeric resolution of the antidepressant drug, citalopram, is presented in this work. N, N'-diethylaminodithiocarbamoylpropyl(trimethoxy)silane has been used as silane iniferter for the surface functionalization of the solid silica support. A molecularly imprinted polymer thin film, in the nm scale, was then grafted on the silanized silica using itaconic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker in the presence of the template S-citalopram. The total monomer amount was calculated to obtain the desired thickness. Non-imprinted stationary phases were prepared similarly in the absence of S-citalopram. Characterization of the materials was carried out by scanning electron microscopy, thermogravimetric analysis, elemental analysis and Fourier transform infrared spectroscopy. Stationary phases have been applied to the chromatographic separation of the target. Conditions for best chromatographic resolution of the enantiomers were optimized, and it was found that a mobile phase consisting of a mixture of formate buffer (40 mM, pH 3) and acetonitrile (30:70 v/v) at 40 °C provided best results. Binding behaviour of the developed material was finally assessed by batch rebinding experiments. The obtained binding isotherm was fitted to different binding models being the Freundlich-Langmuir model, the one that best fitted the experimental data. The developed material has shown high selectivity for the target enantiomer, and the stationary phase could be undoubtedly exploited for chiral separation of the drug.

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The Use of Molecularly Imprinted Polymers for Sampling and Sample Preparation

Cited by 10 publications

References 218 publications

Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

Role of Comonomers in the Recognition of Anionic Biomolecules in Water: Hydrogen-Bonded Imprinted Polymeric Receptor

Tailored Polymer-Based Selective Extraction of Lipid Mediators from Biological Samples

Iniferter‐mediated grafting of molecularly imprinted polymers on porous silica beads for the enantiomeric resolution of drugs

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