The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy

Ran, Gai; Feng, Xi-lin; Xie, Yilin; Zheng, Qin; Guo, Peng-peng; Yang, Ming; Feng, Yuan; Chen, Ling; Zhu, Li; Chen, Zhong

doi:10.1016/j.joim.2021.09.004

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…PEI-mediated triple-plasmid transfection method was used to produce rAAV vectors, according to a published protocol. 44 Briefly, HEK293 cells from twenty 15-cm culture plates were harvested at 72 h post transfection. The cell pellet was resuspended in 5 mL of RB TMS Buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0), followed by three repeats of freeze in dry-ice-ethanol bath for 10 min and thaw at 37°C for 10 min, to prepare the crude lysate.…”

Section: Methodsmentioning

confidence: 99%

Improved gene therapy for MFRP deficiency-mediated retinal degeneration by knocking down endogenous bicistronic Mfrp and Ctrp5 transcript

Tian

Zheng

Xie

et al. 2023

Molecular Therapy - Nucleic Acids

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Section: Methodsmentioning

confidence: 99%

Improved gene therapy for MFRP deficiency-mediated retinal degeneration by knocking down endogenous bicistronic Mfrp and Ctrp5 transcript

Tian

Zheng

Xie

et al. 2023

Molecular Therapy - Nucleic Acids

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“…Emerging therapeutic approaches such as gene therapy may have potential in the treatment of HCC; relevant experimental studies have reported significant reductions in HCC cell proliferation without affecting normal hepatocyte function. 6,7 Nevertheless, the prognosis of advanced HCC remains poor, highlighting the ongoing challenges related to improving outcomes in patients with HCC. Thus, methods for early diagnosis and prognosis prediction of HCC warrant development.…”

Section: Introductionmentioning

confidence: 99%

A Novel Four-Gene Signature Based on Nonsense-Mediated RNA Decay for Predicting Prognosis in Hepatocellular Carcinoma: Bioinformatics Analysis and Functional Validation

Zhao,

Wang,

Zhao

et al. 2024

JHC

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Nonsense-mediated RNA decay (NMD), a surveillance pathway for selective degradation of aberrant mRNAs, is associated with cancer progression. Its potential as a predictor for aggressive hepatocellular carcinoma (HCC) is unclear. Here, we present an innovative NMD risk model for predicting HCC prognosis. Methods: The Cancer Genome Atlas (TCGA) data of 374 liver HCC (LIHC) and 50 normal liver samples were extracted. A risk model based on NMD-related genes was developed through least absolute shrinkage and selection operator Cox (LASSO-Cox) regression of the LIHC-TCGA data. Prognostic validation was done using GSE54236, GSE116174, and GSE76427 data. Univariate and multivariate Cox regression analyses were conducted to assess the prognostic value of the model. We also constructed nomograms for survival prediction. Tumor immune infiltration was evaluated using the CIBERSORT algorithm, and the tumor cell phenotype was assessed. Finally, mouse experiments verified UPF3B knockdown effects on HCC tumor characteristics. Results:We developed a risk model based on four NMD-related genes (PABPC1, RPL8, SMG5, and UPF3B) and validated it using GSE54236, GSE116174, and GSE76427 data. The model effectively distinguished high-and low-risk groups corresponding to unfavorable and favorable HCC outcomes. Its prognostic prediction accuracy was confirmed through time-dependent ROC analysis, and clinical-use nomograms with calibration curves were developed. Single-cell RNA sequencing results indicated significantly higher expression of SMG5 and UPF3B in tumor cells. Knockdown of SMG5 and UPF3B inhibited HCC cell proliferation, invasion, and migration, while affecting cell-cycle progression and apoptosis. In vivo, UPF3B knockdown delayed tumor growth and increased immune cell infiltration. Conclusion: Our NMD-related gene-based risk model can help identify therapeutic targets and biomarkers for HCC. Additionally, it assists clinicians in predicting the prognosis of HCC patients.

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The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors both in vitro and in vivo

Xie

Wang

et al. 2023

Journal of Integrative Medicine

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The use of miR122 and its target sequence in adeno-associated virus-mediated trichosanthin gene therapy

Cited by 4 publications

References 39 publications

Improved gene therapy for MFRP deficiency-mediated retinal degeneration by knocking down endogenous bicistronic Mfrp and Ctrp5 transcript

Improved gene therapy for MFRP deficiency-mediated retinal degeneration by knocking down endogenous bicistronic Mfrp and Ctrp5 transcript

A Novel Four-Gene Signature Based on Nonsense-Mediated RNA Decay for Predicting Prognosis in Hepatocellular Carcinoma: Bioinformatics Analysis and Functional Validation

The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors both in vitro and in vivo

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