The use of microscale processing technologies for quantification of biocatalytic Baeyer‐Villiger oxidation kinetics

Doig, Steven D.; Pickering, Samuel C.R.; Lye, Gary J.; Woodley, John M.

doi:10.1002/bit.10344

Cited by 60 publications

(64 citation statements)

References 17 publications

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“…These approaches cover the search for strains expressing enzymes with a higher activity or enantioselectivity [7±9], the optimization of bioconversion processes by screening for high product formation rates [10,11] or substrate screening experiments for the development of fermentation processes [12]. Being only excerpts from a wide variety of miniaturization or automation attempts in bioprocess development these examples clearly show the high potential of HTS techniques.…”

Section: Introductionmentioning

confidence: 99%

High Throughput Screening of Chromatographic Phases for Rapid Process Development

et al. 2005

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The necessity of being ªfirst on the marketº paired with the need to cut production costs highlights the need for strategies and techniques allowing a fast process development while at the same time covering a high number of potential process parameters in order to reach an optimal process sequence. This can only be met by the use of high throughput screening (HTS) techniques. In this paper HTS applications presently applied in bioprocess development are reviewed, their use in chromatographic process parameter evaluation is discussed and concepts for the rational design of HTS experiments are presented. A rational approach for chromatographic parameter screening comprehends an initial screening procedure for ligand selection followed by the incorporation of specific adsorbent features using HTS pipetting systems finalized by a fine tuning step using packed mini-columns. The analytical part of these screening techniques clearly sets the bottleneck of these procedures with a trade off between analytical information and analysis time. Finally, intelligent HTS strategies should in addition include efficient data processing paired with modern tools for design of experiments or model based process design.

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Section: Introductionmentioning

confidence: 99%

High Throughput Screening of Chromatographic Phases for Rapid Process Development

et al. 2005

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“…The approach has also been applied to bioconversion where the relative absence of colloidal contaminants that occur in downstream processing is a benefit (Doig et al, 2002;Lye et al, 2003). In a few instances these studies have been extended to more complete processes aided by the use of robotic systems for moving microwell plates, pipetting and automatically centrifuging the plates (Nealon et al, 2005).…”

Section: Microwell-based Studiesmentioning

confidence: 99%

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Titchener‐Hooker

Dunnill

Hoare

2008

Biotech & Bioengineering

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The article examines how a small set of easily implemented micro biochemical engineering procedures combined with regime analysis and bioprocess models can be used to predict industrial scale performance of biopharmaceutical protein downstream processing. This approach has been worked on in many of our studies of individual operations over the last 10 years and allows preliminary evaluation to be conducted much earlier in the development pathway because of lower costs. It then permits the later large scale trials to be more highly focused. This means that the risk of delays during bioprocess development and of product launch are reduced. Here we draw the outcomes of this research together and illustrate its use in a set of typical operations; cell rupture, centrifugation, filtration, precipitation, expanded bed adsorption, chromatography and for common sources, E. coli, two yeasts and mammalian cells (GS-NSO). The general approach to establishing this method for other operations is summarized and new developments outlined. The technique is placed against the background of the scale-down methods that preceded it and complementary ones that are being examined in parallel. The article concludes with a discussion of the advantages and limitations of the micro biochemical engineering approach versus other methods.

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“…Growth was followed off-line by OD (640 nm) and dry weight, applying a sacrificial well approach in the case of the MTP [20].…”

Section: Siderophore Productionmentioning

confidence: 99%

Anchoring high-throughput screening methods to scale-up bioproduction of siderophores

Marques

Walshe

Doyle

et al. 2012

Process Biochemistry

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a b s t r a c tThe use of high throughput strategies is of acknowledged relevance since the rational use of small-scale reactors, coupled with suitable analytic tools, is contributing to the acceleration of process development in several areas of biotechnology. These small-scale reactors are available in different working volumes and configurations, being useful in a wide array of applications, from cell screening to process optimization.The present work was focused on the development of a high-throughput strategy, combining microtiter plates and analytic methodologies, to screen an in-house library of environmental bacteria in order to identify good siderophore producers. From a library of roughly 500 marine microorganisms, it was possible to ultimately obtain 11 bacterial strains with high production capabilities. Two of them had not been previously identified as siderophore producers. The bioprocess was scaled-up from microtiter plates to a 5 L stirred tank reactor, while maintaining the overall volumetric productivity, using the k L a similarity as scale-up criterion.This novel approach is a suitable alternative to traditional screening tools.

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The use of microscale processing technologies for quantification of biocatalytic Baeyer‐Villiger oxidation kinetics

Cited by 60 publications

References 17 publications

High Throughput Screening of Chromatographic Phases for Rapid Process Development

High Throughput Screening of Chromatographic Phases for Rapid Process Development

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Anchoring high-throughput screening methods to scale-up bioproduction of siderophores

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