The use of microfluidic devices in studies of differential sperm chemotaxis

Penny, Jake A.; Lymbery, Rowan A.; Evans, Jonathan P.; Sherman, Craig D. H.; Conlan, Xavier A.

doi:10.1016/j.tibtech.2022.06.014

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…Penny et al. [ 58 ] summarize the key challenges and potential solutions applied to differential sperm chemotaxis. They anticipate that advances in this field will have potential applications beyond reproductive and evolutionary biology, including clinical settings.…”

Section: Natural Cells/bacteria‐based Chemotactic Micro/nanomotorsmentioning

confidence: 99%

“…Microfluidic devices provide a highly flexible platform to study many aspects of sperm motility and chemotaxis behavior while enabling researchers to control and experimentally manipulate different environmental conditions. Penny et al [58] summarize the key challenges and potential solutions applied to differential sperm chemotaxis. They anticipate that advances in this field will have potential applications beyond reproductive and evolutionary biology, including clinical settings.…”

Section: Sperm Cellsmentioning

confidence: 99%

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Chemotactic Micro/Nanomotors for Biomedical Applications

Xia,

Li,

Xiao

et al. 2023

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In nature, many organisms respond chemotactically to external chemical stimuli in order to extract nutrients or avoid danger. Inspired by this natural chemotaxis, micro/nanomotors with chemotactic properties have been developed and applied to study a variety of disease models. This chemotactic strategy has shown promising results and has attracted the attention of an increasing number of researchers. This paper mainly reviews the construction methods of different types of chemotactic micro/nanomotors, the mechanism of chemotaxis, and the potential applications in biomedicine. First, based on the classification of materials, the construction methods and therapeutic effects of chemotactic micro/nanomotors based on natural cells and synthetic materials in cellular and animal experiments will be elaborated in detail. Second, the mechanism of chemotaxis of micro/nanomotors is elaborated in detail: chemical reaction induced chemotaxis and physical process driven chemotaxis. In particular, the main differences and significant advantages between chemotactic micro/nanomotors and magnetic, electrical and optical micro/nanomotors are described. The applications of chemotactic micro/nanomotors in the biomedical fields in recent years are then summarized, focusing on the mechanism of action and therapeutic effects in cancer and cardiovascular disease. Finally, the authors are looking forward to the future development of chemotactic micro/nanomotors in the biomedical fields.

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Section: Natural Cells/bacteria‐based Chemotactic Micro/nanomotorsmentioning

confidence: 99%

Section: Sperm Cellsmentioning

confidence: 99%

Chemotactic Micro/Nanomotors for Biomedical Applications

Xia,

Li,

Xiao

et al. 2023

Small

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“…To achieve amplification-free detection, a simple approach is to reduce the reactor to increase the local target concentration. Single-molecule sensitivity was achieved by harnessing an ultralocalized/confined reactor or microvolume, such as through the use of droplet microfluidics, microchamber-array technologies, etc. , This not only improves the reaction efficiency via the confinement effect but also reduces the requirement for manual operation and shortens turnaround times through miniaturization and integration. Besides, this makes multiplexed detection possible …”

Section: Crispr/cas-driven Amplification-free Detectionmentioning

confidence: 99%

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives

Li,

Liu,

Tang

et al. 2023

ACS Sens.

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CRISPR/Cas system is becoming an increasingly influential technology that has been repositioned in nucleic acid detection. A preamplification step is usually required to improve the sensitivity of CRISPR/Cas-based detection. The striking biological features of CRISPR/ Cas, including programmability, high sensitivity and sequence specificity, and single-base resolution. More strikingly, the target-activated trans-cleavage could act as a biocatalytic signal transductor and amplifier, thereby empowering it to potentially perform nucleic acid detection without a preamplification step. The reports of such work are on the rise, which is not only scientifically significant but also promising for futuristic end-user applications. This review started with the introduction of the detection methods of nucleic acids and the CRISPR/ Cas-based diagnostics (CRISPR-Dx). Next, we objectively discussed the pros and cons of preamplification steps for CRISPR-Dx. We then illustrated and highlighted the recently developed strategies for CRISPR/Cas-powered amplification-free detection that can be realized through the uses of ultralocalized reactors, cascade reactions, ultrasensitive detection systems, or others. Lastly, the challenges and futuristic perspectives were proposed. It can be expected that this work not only makes the researchers better understand the current strategies for this emerging field, but also provides insight for designing novel CRISPR-Dx without a preamplification step to win practicable use in the near future.

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