The use of mechano growth factor to prevent cartilage degeneration in knee osteoarthritis

Abstract

Previous studies have attached more importance to growth factors in treating cartilage degeneration and osteoarthritis (OA). Here, the capability of mechano growth factor-C24E (MGF) to prevent osteoarthritic cartilage degeneration was evaluated in vitro and in vivo. Using in vitro cultured human OA chondrocytes treated with 10-60-ng/ml MGF for 12 hr, we detected the cell proliferation, migration, and anabolism of OA chondrocytes. The unfolded protein response and the protein characteristic of OA pathology, such as transforming growth factor β, SMAD family member 3, and hypoxia-inducible factor 2α of OA chondrocytes, were also detected by western blotting. Furthermore, protein kinase RNA-like endoplasmic reticulum kinase was knocked down via small interfering RNA to illuminate the potential mechanism of MGF's treatment of OA. In a rabbit knee joint OA model, cartilage degeneration was inhibited after 2 weeks of treatment with 0.1-10-μg/ml MGF. This study demonstrated that MGF treatment can inhibit the pathological apoptosis of OA chondrocytes and promote the proliferation, migration, and matrix synthesis of the chondrocytes. The results also demonstrate that the degeneration of OA cartilage can be delayed by MGF treatment partially via unfolded protein response regulated by protein kinase RNA-like endoplasmic reticulum kinase and suggest a potential therapeutic application of MGF for OA treatment.

Surface modification of electrospun fibers with mechano-growth factor for mitigating the foreign-body reaction

“…Except to promote cell proliferation and migration, MGF also can regulate cell phenotype transition such as stem cell differentiation [ 43 ]. In regulating immune response, in vivo studies prove that MGF possesses strong anti-inflammation capability in repairing rat tendon injury and preventing rabbit osteoarthritis [ 36 ]. In vitro study further reported that MGF can reduce the pro-inflammatory cytokine expression in human fibroblast-like synoviocytes of knee osteoarthritis such as IL-1β and TNF-α [ 35 ].…”

“…To investigate the role of MGF in preventing knee osteoarthritis, we found that MGF can inhibit inflammatory cytokine production in human synoviocytes of knee osteoarthritis, such as IL-1β and TNF-α [ 35 ]. Additionally, a study on rabbit knee joint osteoarthritis, we found that MGF joint cavity injection will not only prevent cartilage degradation, but also relief the inflammation induced arthrocele [ 36 ]. These findings indicate that MGF not only promotes tissue repair, but also has the potential to regulate the inflammatory microenvironment in vitro and in vivo .…”

Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis

“…All the specimens were separately stained with hematoxylin and eosin (H&E), Safranin-O/ green, and Alcian blue. In the microscopic examinations, the histological scores were rated according to the histologcial assessment table (Table 3) followed from our previous study 21, 22, the score of each section come from the sum of each item. The statistical analysis was based on the mean score for each group.…”

The role of growth factor in the repair of articular cartilage

“…The repair effect of MGF is very similar to that of normal cartilage. Song et al [70] have shown that MGF can stimulate anabolism in the process of OA, and MGF can up-regulate cartilage ECM synthesis genes ColII ACAN SOX9 and HAS. With the increase of MGF dose, the expression of cola, scan and Sox9 increased linearly, indicating that MGF has a stable effect on ECM regeneration and cartilage formation.…”