The Use of Mathematical Modelling for Improving the Tissue Engineering of Organs and Stem Cell Therapy

Lemon, Greg; Sjöqvist, Sebastian; Lim, Mei Ling; Feliu, Neus; Firsova, Alexandra B.; Amin, Risul; Gustafsson, Ylva; Stuewer, Annika; Губарева, Е. А.; Haag, Johannes C.; Jungebluth, Philipp; Macchiarini, Paolo

doi:10.2174/1574888x10666151001115942

Cited by 6 publications

(1 citation statement)

References 85 publications

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“…Here, we present a multidisciplinary approach (figure 1) to address the connected problems of cell survival and revascularization, demonstrating the potential of using mathematical modelling in combination with experimental work to accelerate and direct tissue engineering research [26]. Integrated mathematical-experimental work has a rich history and a promising future in the field of regenerative medicine [27,28] and general biology [29][30][31]. Mathematical modelling offers experimental scientists a tool to extract further information from their data.…”

Section: Introductionmentioning

confidence: 99%

Combiningin silicoandin vitromodels to inform cell seeding strategies in tissue engineering

Coy

Al-Badri

Kayal

et al. 2020

J. R. Soc. Interface.

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The seeding density of therapeutic cells in engineered tissue impacts both cell survival and vascularization. Excessively high seeded cell densities can result in increased death and thus waste of valuable cells, whereas lower seeded cell densities may not provide sufficient support for the tissue in vivo , reducing efficacy. Additionally, the production of growth factors by therapeutic cells in low oxygen environments offers a way of generating growth factor gradients, which are important for vascularization, but hypoxia can also induce unwanted levels of cell death. This is a complex problem that lends itself to a combination of computational modelling and experimentation. Here, we present a spatio-temporal mathematical model parametrized using in vitro data capable of simulating the interactions between a therapeutic cell population, oxygen concentrations and vascular endothelial growth factor (VEGF) concentrations in engineered tissues. Simulations of collagen nerve repair constructs suggest that specific seeded cell densities and non-uniform spatial distributions of seeded cells could enhance cell survival and the generation of VEGF gradients. These predictions can now be tested using targeted experiments.

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Section: Introductionmentioning

confidence: 99%

Combiningin silicoandin vitromodels to inform cell seeding strategies in tissue engineering

Coy

Al-Badri

Kayal

et al. 2020

J. R. Soc. Interface.

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Scaffold-free: A developing technique in field of tissue engineering

Alblawi

Ranjani

Yasmin

et al. 2020

Computer Methods and Programs in Biomedicine

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Effects of nutrient concentration and scaffold elasticity on the tissue growth in a tissue engineering scaffold channel

Fattahpour,

Sanaei

2023

Physics of Fluids

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Tissue-engineering scaffolds contain channels lined by cells that allow nutrient-rich culture medium to pass through to encourage cell proliferation. Several factors have significant impacts on the tissue growth, including the nutrient flow rate, concentration in the feed, scaffold elasticity, and cell properties. Recent studies have investigated these effects separately; however, in this work, we examine all of them simultaneously. Our objectives in this work are as follows: (i) developing a mathematical model describing the nutrient flow dynamics and concentration, scaffold elasticity, and cell proliferation; (ii) solving the model and then simulating the cell proliferation process; and (iii) optimizing the initial configuration of the scaffold channels to maximize the cell growth. The results of our study demonstrate that the rate of nutrient consumption by the cells (cell hunger rate) and the scaffold elastic compliance have an impact on tissue growth, with higher cell hunger rates leading to longer incubation periods, while scaffold elastic compliance slightly affects overall growth. Furthermore, decreasing the scaffold elastic compliance while maintaining a constant nutrient consumption rate results in an optimal funnel-shaped channel geometry, where the upper part of the channel is larger than the downstream, promoting enhanced tissue integration and functionality.

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The Use of Mathematical Modelling for Improving the Tissue Engineering of Organs and Stem Cell Therapy

Cited by 6 publications

References 85 publications

Combiningin silicoandin vitromodels to inform cell seeding strategies in tissue engineering

Combiningin silicoandin vitromodels to inform cell seeding strategies in tissue engineering

Scaffold-free: A developing technique in field of tissue engineering

Effects of nutrient concentration and scaffold elasticity on the tissue growth in a tissue engineering scaffold channel

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